Article
Engineering, Chemical
Fangmeng Sheng, Xingya Li, Yuanyuan Li, Noor Ul Afsar, Zhang Zhao, Liang Ge, Tongwen Xu
Summary: A cationic nanoporous covalent organic framework (COF) membrane was successfully constructed using a simple method. The membrane showed excellent performance in dye/salt separation with high pure water flux. The rejection of dyes was mainly influenced by electrostatic repulsion and size sieving effect.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Chemistry, Physical
Shuqing Sun, Yu Xia, Jiaojiao Liu, Yujiang Dou, Kai Yang, Bing Yuan, Zhenhui Kang
Summary: This study combined photo-voltage transient technique and dynamic giant bistratal vesicle (GBV) leakage method to monitor the real-time and molecule-level interaction between TAT and cell membrane. The results showed that TAT caused disturbance and damage to the membrane, and affected the membrane permeability. It was also found that the membrane immobilized on the substrate had a stronger permeabilizing effect on TAT.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jordi Marti, Huixia Lu
Summary: This study investigates the interactions of tryptophan, serotonin, and melatonin with zwitterionic phospholipid bilayers through molecular dynamics simulations. The presence of cholesterol influences the behavior of these small molecules, affecting their binding to the membrane. Free energy barriers and diffusion rates of the small molecules provide insights into their interaction with phospholipids and cholesterol in the membrane environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Elisa Aranda, Jose A. Teruel, Antonio Ortiz, Maria Dolores Perez-Carceles, Jose N. Rodriguez-Lopez, Francisco J. Aranda
Summary: The anticarcinogenic properties of catechins, their interaction with lipids, and their potential site of action in membranes were studied. Experimental and computational research showed that catechins can modify lipid polymorphism and membrane structure and dynamics. These findings provide insights into the molecular effects of catechins and their potential impact on membrane function.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Review
Chemistry, Medicinal
Katsumi Matsuzaki
Summary: This review summarizes the major research carried out in the author's laboratory on how lipids and their inhomogeneous distributions regulate the structures and functions of antimicrobial peptides and Alzheimer's amyloid beta-protein. Also, how to detect transmembrane helix-helix and membrane protein-protein interactions and how they are modulated by lipids are discussed.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Crystallography
Qingfeng Wu, Kristen Carlson, Qi Cheng, Xisen Wang, Zhaohui Li
Summary: The interactions between cationic dyes and negatively charged mineral surfaces, specifically palygorskite and sepiolite, were investigated in this study. It was found that the dimer molecules formed on the surface of the minerals contributed to the higher TB uptake in addition to cation exchange. Palygorskite was shown to be a better carrier for cationic dyes due to its limited SSA and large CEC values, while sepiolite had a loosely dimeric formation due to its large SSA and limited CEC values.
Article
Chemistry, Multidisciplinary
Shuai Liu, Xu Wang, Xueliang Yu, Qiang Cheng, Lindsay T. Johnson, Sumanta Chatterjee, Di Zhang, Sang M. Lee, Yehui Sun, Ting-Chih Lin, John L. Liu, Daniel J. Siegwart
Summary: The study introduces a rational design and combinatorial synthesis of zwitterionic phospholipidated polymers (ZPPs) for efficient mRNA delivery to spleen and lymph nodes, with potential for substantial immunotherapeutic applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Engineering, Chemical
Chen Zhao, Yahan Ye, Xianfu Chen, Xiaowei Da, Minghui Qiu, Yiqun Fan
Summary: This study modifies a ceramic membrane by grafting aminosilane to enhance its rejection performance towards methylene blue solution, and provides guidance for treating industrial cationic dye wastewater.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2022)
Article
Soil Science
Jinhong He, Xiangping Tan, Yanxia Nie, Lei Ma, Weiping Zhou, Weijun Shen
Summary: Research found that forest soil microorganisms acclimate to temperature variations by changing the lipid composition of their cell membranes. Saturated fatty acids increase while unsaturated fatty acids decrease with increasing temperature. The long-term warming may result in larger alterations in lipid constitute of soil microbial membranes compared to short-term incubation temperature gradient.
SOIL BIOLOGY & BIOCHEMISTRY
(2023)
Article
Plant Sciences
Rosa L. Lopez-Marques, James A. Davis, Jeffrey F. Harper, Michael Palmgren
Summary: The lipid bilayer of biological membranes is complex and asymmetric, with protein P4 ATPase playing a role in generating this asymmetry. Arabidopsis contains 12 P4 ATPases, with studies confirming their essential role in basal cellular functions.
Article
Chemistry, Physical
Mariana R. N. Lima, Kim-Phuong N. Le, Daniel Chakhalian, Yong Mao, Joachim Kohn, David I. Devore
Summary: The design of biodegradable tyrosine-derived polymeric surfactants (TyPS) using calculated thermodynamic parameters can control cellular properties such as viability. Delivery of cholesterol by TyPS nanospheres into membrane phospholipid domains can further control membrane properties.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Beata Tim, Monika Rojewska, Krystyna Prochaska
Summary: This paper investigates the mechanism of interaction between inhaled pollutant microparticles and cell membrane components, focusing on the impact of Chol molecules on the surface properties of a model membrane. The results show that Chol causes condensation of the DPPC monolayer, influencing the penetration of MPs and their interactions with the model membrane.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Alice Spadea, Mark Jackman, Lili Cui, Sara Pereira, M. Jayne Lawrence, Richard A. Campbell, Marianne Ashford
Summary: This study investigates the mechanism of interaction between nucleic acid-loaded LNPs and endosomal membranes using different reflectometry techniques. The insertion of lipids from LNPs into the model membrane is found to be greatest at pH 6.5 and 5.5, while at higher pH, lipid insertion is suppressed and intact LNPs are bound instead. The stage of the endosome has minimal effect on the extent of LNP interaction. The nature of the nucleic acid cargo can be tuned to optimize the interaction and improve nucleic acid delivery by LNPs.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Medicinal
Syusuke Okano, Yoshimasa Kawaguchi, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Shiroh Futaki
Summary: This study aimed to estimate the cytosolic concentrations of intracellularly delivered peptides and proteins and provide insights for the design of novel and effective biopharmaceutical delivery systems. The researchers utilized the split NanoLuc luciferase system and HiBiT peptide segment as a probe to measure the cytosolic HiBiT concentration. They found that L17E and HAad peptides significantly increased the cellular HiBiT concentrations.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Pengzhen Zhang, Fangfang Jiao, Lingxiao Wu, Zhe Kong, Wei Hu, Lijun Liang, Yongjun Zhang
Summary: This study investigates the permeation mechanisms of graphene quantum dots (GQDs) through different cell membranes using molecular dynamics simulations. The results show that GQDs can easily penetrate lipid membranes with low phospholipid molecule densities, but are unable to penetrate those with high densities. The study provides insights into the transmembrane transport of GQDs.
Article
Polymer Science
Nazmul Hasan, Christian Schwieger, Hisaschi T. Tee, Frederik R. Wurm, Karsten Busse, Joerg Kressler
EUROPEAN POLYMER JOURNAL
(2018)
Article
Chemistry, Multidisciplinary
Geert A. Daudey, Christian Schwieger, Martin Rabe, Alexander Kros
Article
Chemistry, Multidisciplinary
Sindy Mueller, Christian Schwieger, Kai Gruhle, Vasil M. Garamus, Gerd Hause, Annette Meister, Simon Drescher
Article
Chemistry, Multidisciplinary
Simone A. G. Langeveld, Christian Schwieger, Ines Beekers, Jacob Blaffert, Tom van Rooij, Alfred Blume, Klazina Kooiman
Article
Biochemistry & Molecular Biology
Syed W. H. Shah, Christian Schwieger, Joerg Kressler, Alfred Blume
CHEMISTRY AND PHYSICS OF LIPIDS
(2020)
Article
Polymer Science
Nazmul Hasan, Christian Fuchs, Christian Schwieger, Karsten Busse, Oleksandr Dolynchuk, Joerg Kressler
Article
Chemistry, Multidisciplinary
Matthias Hoffmann, Simon Drescher, Christian Ihling, Dariush Hinderberger, Christian Schwieger
Article
Chemistry, Multidisciplinary
Melissa Frick, Christian Schwieger, Carla Schmidt
Summary: Membrane proteins, crucial for cell function, can be effectively studied using liposomes as membrane mimetics for mass spectrometric analysis. Liposomes, advantageous for their ease of preparation and ability to vary in size and composition, have been shown to dissociate into lipid clusters in the gas phase of a mass spectrometer while retaining intact protein and protein-lipid complexes. This approach opens the door for the widespread application of liposomes in mass spectrometric analysis of membrane-associated proteins.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biophysics
Jan Auerswald, Jan Ebenhan, Christian Schwieger, Andrea Scrima, Annette Meister, Kirsten Bacia
Summary: Membrane insertion of protein domains is crucial for various membrane remodeling processes. This study presents a novel method for directly measuring the number of inserted proteins and total area increase in lipid monolayers using two-color fluorescence correlation spectroscopy. The results obtained for Sar1 in COPII reconstitution experiments are in agreement with the expected insertion area of the Sar1 amphipathic helix.
BIOPHYSICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Julia Dorner, Patricia Korn, Kai Gruhle, Daniel Ramsbeck, Vasil M. Garamus, Hauke Lilie, Annette Meister, Christian Schwieger, Christian Ihling, Andrea Sinz, Simon Drescher
Summary: This study examines the potential of diazirine-modified lipids for lipid/peptide interactions research, revealing unexpected backfolding behavior of the diazirine-containing stearoyl chain in the lipid. This finding challenges the application of DiazPC for future studies on peptide and protein/lipid interactions.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Multidisciplinary Sciences
Kang-Cheng Liu, Hudson Pace, Elin Larsson, Shakhawath Hossain, Aleksei Kabedev, Ankita Shukla, Vanessa Jerschabek, Jagan Mohan, Christel A. S. Bergstrom, Marta Bally, Christian Schwieger, Madlen Hubert, Richard Lundmark
Summary: Caveolae, important plasma membrane invaginations, are shaped by the coat protein Cavin1. This study reveals the mechanism of Cavin1 assembly at the membrane interface, involving initial PI(4,5)P-2-dependent membrane adsorption, subsequent partial separation and membrane insertion. This intricate mechanism facilitates membrane curvature generation and dynamic assembly-disassembly of Cavin1 at the membrane.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Patricia Korn, Christian Schwieger, Kai Gruhle, Vasil M. Garamus, Annette Meister, Christian Ihling, Simon Drescher
Summary: This study compared the properties and reactivity of saturated phospholipids mixed with azide- or diazirine-modified lipids. The results showed that both photo-reactive lipids can be used to study lipid/peptide and lipid/protein interactions, but they both have the issue of backfolding.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Chemistry, Physical
Matthias Hoffmann, Simon Drescher, Christian Schwieger, Dariush Hinderberger
Summary: By studying surface pressure-molecular area isotherms and infrared reflection absorption spectroscopy of phospholipid monolayers, it was found that replacing an ester linkage with an ether linkage significantly impacts headgroup hydration and ordering effects. Analysis of mixed monolayers showed a non-ideal mixing behavior of DPPC and PHPC, potentially resulting from conformational differences in their headgroups.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Meeting Abstract
Biophysics
Christian Schwieger
BIOPHYSICAL JOURNAL
(2020)
Meeting Abstract
Biophysics
J. Auerswald, J. Ebenhan, C. Schwieger, A. Scrima, A. Meister, K. Bacia
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2019)
Article
Biochemistry & Molecular Biology
Hsiao-Chieh Tsai, Ching-Hong Huang, Ling-Hsien Tu
Summary: Islet amyloid polypeptide (IAPP) is a polypeptide hormone co-secreted with insulin by pancreatic beta-cells. It tends to aggregate into soluble oligomers, which are considered one of the hallmarks of type II diabetes. This study successfully grafted the aggregation-induced emission molecule TPE onto IAPP, allowing real-time monitoring of IAPP oligomer formation and potential application in the diagnosis of T2D.
BIOPHYSICAL CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Aristeidis Papagiannopoulos, Aggeliki Sklapani, Nikolaos Spiliopoulos
Summary: This study presents a method for preparing Hb-based nanoparticles (NPs) using a fully biocompatible approach. These NPs have a spherical structure with a diameter ranging from 50 to 100 nm, and can form electrostatic complexes with CS at pH 4. The NPs can be pH-tunable and stable in solutions with high salt content, making them suitable for nanodelivery of nutrients and drugs.
BIOPHYSICAL CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Andrey V. Struts, Alexander V. Barmasov, Steven D. E. Fried, Kushani S. K. Hewage, Suchithranga M. D. C. Perera, Michael F. Brown
Summary: This article summarizes and reviews the osmotic stress studies of G-protein-coupled receptor rhodopsin. It is found that water plays an important role in the activation of the receptor, with at least 80 water molecules entering the receptor in the transition to the active state. If water influx is prevented, the functional transition of the receptor is reversed. These findings reveal the phenomenon of solvent swelling in the activation mechanism of rhodopsin, with water acting as an allosteric modulator of function for rhodopsin-like receptors in lipid membranes.
BIOPHYSICAL CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Maria Chiara Saija, Adela Melcrova, Wojciech Pajerski, Itay Schachter, Matti Javanainen, Marek Cebecauer, Lukasz Cwiklik
Summary: We used molecular dynamics simulations to investigate the effects of palmitoylation on a transmembrane peptide in different lipid environments. The study found that palmitoylation reduces the peptide's impact on membrane thickness, particularly in lipid-ordered and boundary environments. The hydrophobic palmitoyl chains on the peptide did not significantly affect membrane hydration. Interestingly, the boundary membrane environment was found to be highly compatible with the palmitoylated peptide. These findings have important implications for understanding cell signaling, membrane organization, and optimizing lipid membrane-based drug delivery systems.
BIOPHYSICAL CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Achanta Rishisree, Brayer Mallory, Karnaukhova Elena, Jankovic Teodora, Zdunic Gordana, Savikin Katarina, Jeremic Aleksandar
Summary: Pomegranate peel, ironwort, and chokeberry leaf extracts exhibit anti-aggregative and antitoxic properties against human amylin. They can prevent amyloidosis and cell loss in patients with Type 2 Diabetes Mellitus.
BIOPHYSICAL CHEMISTRY
(2024)