Article
Biochemical Research Methods
Jingqi Qian, Mian Wang, Zhaoxiang Wang, Rui Feng, Jiaqi Zhang, Chencheng Ye, Man Zhang, Baomin Wang, Liwang Cui
Summary: This study developed antibody-based assays for the detection and quantitation of the antimalarial drug Amodiaquine (AQ) without requiring sophisticated equipment. The developed methods were successfully applied for quality control of AQ-containing drugs and showed comparable results to high-performance liquid chromatography.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Lijiao Kan, Edoardo Capuano, Vincenzo Fogliano, Ruud Verkerk, Jurriaan J. Mes, Monic M. M. Tomassen, Teresa Oliviero
Summary: The study showed that tea polyphenols have inhibitory effects on alpha-glucosidase activity in both rat intestinal enzymes and human Caco-2 cells, but the specific effects varied depending on the type of tea extract and the enzyme being targeted.
Article
Pharmacology & Pharmacy
Fengfeng Qu, Zeyi Ai, Shuyuan Liu, Haojie Zhang, Yuqiong Chen, Yaomin Wang, Dejiang Ni
Summary: This study revealed that the low bioavailability of theaflavins in Caco-2 monolayers is attributed to their structural instability, efflux transporters, and cell metabolism.
Article
Food Science & Technology
Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu
Summary: In this study, it was found that Ganoderma atrum polysaccharide (PSG-1) indirectly affects dendritic cells' immune function in the intestinal-like Caco-2/DCs co-culture model. The interaction between Caco-2 and DCs was regulated by PSG-1 through TNF-alpha and NF-kappa B signaling pathways of Caco-2 in the model. This suggests a potential mechanism for immune modulation in the gut environment through interactions between intestinal epithelial cells and immune cells.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Pharmacology & Pharmacy
Kazuyoshi Michiba, Kazuya Maeda, Ko Kurimori, Tsuyoshi Enomoto, Osamu Shimomura, Tomoyo Takeuchi, Hiroyuki Nishiyama, Tatsuya Oda, Hiroyuki Kusuhara
Summary: This study characterized the functions of major intestinal uptake/efflux drug transporters in freshly isolated human jejunum sections using the Ussing chamber system. The findings showed a good correlation between the mucosal-to-serosal apparent permeability coefficient of various drugs and reported human FaFg values, highlighting the importance of intestinal uptake transporters in facilitating drug absorption in humans.
DRUG METABOLISM AND DISPOSITION
(2021)
Article
Multidisciplinary Sciences
Zachary M. Slifer, Liliana Hernandez, Tiffany A. Pridgen, Alexandra R. Carlson, Kristen M. Messenger, Jay Madan, B. Radha Krishnan, Sandeep Laumas, Anthony T. Blikslager
Summary: Intestinal ischemia leads to mucosal injury, including loss of paracellular barrier due to disruption of tight junctions. Larazotide acetate (LA), a small peptide under Phase III clinical trials for celiac disease, accelerates recovery of ischemic injured tissue by modulating tight junctions. An optimal dose of 1µM LA significantly increases transepithelial electrical resistance and reduces LPS flux, with evidence of inhibition by LA fragments.
Article
Chemistry, Analytical
Giuseppe Perna, Vito Capozzi, Maria Lasalvia
Summary: This study found that exposure to low concentrations of pesticide residues can lead to biochemical changes in intestinal cells, particularly an increase in the ratio of lipid to protein components. Raman micro-spectroscopy can be successfully used to monitor these cellular modifications.
Article
Biology
Ross T. Lindsay, Sophie Dieckmann, Dominika Krzyzanska, Dominic Manetta-Jones, James A. West, Cecilia Castro, Julian L. Griffin, Andrew J. Murray
Summary: Under conditions of low-flow ischemia, the heart can generate ketones through pathways involving HMGCS2 and SCOT, impacting cardiac functional recovery.
Article
Physiology
Kei Amari, Ryo Kajihara, Nariaki Arai, Moyuru Hayashi, Tomomi Watanabe-Asaka, Maki Kaidoh, Yumiko Yokoyama, Kumiko Ajima, Daisuke Maejima, Yoshiko Kawai, Toshio Ohhashi
Summary: This study aimed to clarify the mechanisms involved in the regulation of jejunal lymph formation. The results showed that serotonin and water intake significantly increased the mesenteric lymph volume with permeant albumin in the jejunal microcirculation. Intravenous administration of L-NAME pretreatment reduced the responses induced by serotonin and water intake. Intravenous administration of L-NAME itself also decreased the jejunal lymph formation. Additionally, administration of A23187 and ML-7 significantly reduced the jejunal lymph formation with permeant albumin, while nicardipine administration increased lymph formation.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ibitamuno Caleb, Luca Erlitz, Vivien Telek, Monika Vecsernyes, Gyorgy Setalo, Peter Hardi, Ildiko Takacs, Gabor Jancso, Tibor Nagy
Summary: Cold storage induces changes in intestinal autophagy, with enhanced autophagy providing protection against cold ischemia-reperfusion damage to the small bowels.
Article
Operations Research & Management Science
Xuan Vinh Doan, Stephen Vavasis
Summary: The study presents novel Ky Fan 2-k-norm-based models for non-convex low-rank matrix recovery problems, along with a difference of convex functions algorithm and a proximal point algorithm framework for solving them. Numerical results demonstrate high recoverability rates for large instances using the proposed approach.
JOURNAL OF GLOBAL OPTIMIZATION
(2022)
Article
Chemistry, Medicinal
Qiong Yang, Maochen Xing, Ke Wang, Qiang Wei, Jiarui Zhao, Yuan Wang, Kai Ji, Shuliang Song
Summary: Fucoidan can be used to shorten the establishment period of the Caco-2 model to 5 days without affecting the relevant indicators.
Article
Nutrition & Dietetics
Agnes Klusoczki, Boglarka Olah, Dominik Hosszu, Ferenc Fenyvesi, Judit Remenyik, Judit Homoki, Alexandra Gyongyosi, Ildiko Bacskay, Judit Varadi
Summary: This study aimed to investigate the interaction between anthocyanins and gliadin and the impact of anthocyanins on barrier modulation in Caco-2 cells. The results showed that anthocyanins were able to reduce the release of cytokines and mitochondrial ROS levels, exhibiting anti-inflammatory, stabilizing, and restoring effects.
Article
Environmental Sciences
Jiangbing Qiu, Jingrui Zhang, Aifeng Li
Summary: This review summarizes the application of the Caco-2 cell monolayer model in assessing the uptake and damage of phycotoxins in the intestine. Previous studies have shown that different groups of phycotoxins exhibit varying cytotoxicity and transport mechanisms in Caco-2 cells, suggesting the need for the development of multicellular intestinal models for comprehensive toxicity and absorption studies.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Food Science & Technology
Jerome Henri, Rachelle Lanceleur, Jean-Michel Delmas, Valerie Fessard, Antoine Huguet
Summary: Microcystins are toxins produced by cyanobacteria globally, with variations in their structure affecting toxicity. Research indicates that oral absorption of Microcystin-RR may be poor, despite its common structure with more toxic variants like Microcystin-LR.
Article
Genetics & Heredity
Nyarai Desiree Soko, Emile Chimusa, Collen Masimirembwa, Collet Dandara
PHARMACOGENOMICS JOURNAL
(2019)
Editorial Material
Pharmacology & Pharmacy
Nyarai Desiree Soko, Collen Masimirembwa, Collet Dandara
Article
Public, Environmental & Occupational Health
Jean H. Humphrey, Mduduzi N. N. Mbuya, Robert Ntozini, Lawrence H. Moulton, Rebecca J. Stoltzfus, Naume V. Tavengwa, Kuda Mutasa, Florence Majo, Batsirai Mutasa, Goldberg Mangwadu, Cynthia M. Chasokela, Ancikaria Chigumira, Bernard Chasekwa, Laura E. Smith, James M. Tielsch, Andrew D. Jones, Amee R. Manges, John A. Maluccio, Andrew J. Prendergast
LANCET GLOBAL HEALTH
(2019)
Article
Pharmacology & Pharmacy
Zeruesenay Desta, Roseann S. Gammal, Li Gong, Michelle Whirl-Carrillo, Aditya H. Gaur, Chonlaphat Sukasem, Jennifer Hockings, Alan Myers, Marelize Swart, Rachel F. Tyndale, Collen Masimirembwa, Otito F. Iwuchukwu, Sanika Chirwa, Jeffrey Lennox, Andrea Gaedigk, Teri E. Klein, David W. Haas
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2019)
Article
Pharmacology & Pharmacy
Lovejoy Nleya, Roslyn Thelingwani, Xue-Qing Li, Erika Cavallin, Emre Isin, Charles Nhachi, Collen Masimirembwa
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Lonji Kalombo, Yolandy Lemmer, Boitumelo Semete-Makokotlela, Bathabile Ramalapa, Patric Nkuna, Laetitia L. L. I. J. Booysen, Saloshnee Naidoo, Rose Hayeshi, Jan A. Verschoor, Hulda S. Swai
Article
Chemistry, Analytical
John Takyi-Williams, Linne Erasmus, Rose Hayeshi, Anne Grobler
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Review
Pharmacology & Pharmacy
Fouzia Radouani, Lyndon Zass, Yosr Hamdi, Jorge da Rocha, Reem Sallam, Sonia Abdelhak, Samah Ahmed, Maryame Azzouzi, Ichrak Benamri, Alia Benkahla, Balkiss Bouhaouala-Zahar, Melek Chaouch, Haifa Jmel, Rym Kefi, Ayoub Ksouri, Judit Kumuthini, Phumlani Masilela, Collen Masimirembwa, Houcemeddine Othman, Sumir Panji, Lilia Romdhane, Chaimae Samtal, Rania Sibira, Kais Ghedira, Faisal Fadlelmola, Samar Kamal Kassim, Nicola Mulder
PERSONALIZED MEDICINE
(2020)
Article
Multidisciplinary Sciences
Joconiah Chirenda, Isaiah Gwitira, Robin M. Warren, Samantha L. Sampson, Amon Murwira, Collen Masimirembwa, Kudzanai M. Mateveke, Cremence Duri, Prosper Chonzi, Simbarashe Rusakaniko, Elizabeth M. Streicher
Article
Pharmacology & Pharmacy
Oluwatoyin A. Adeleke, Rose K. Hayeshi, Hajierah Davids
Article
Biochemistry & Molecular Biology
Vusani Mandiwana, Lonji Kalombo, Rose Hayeshi, Jan Rijn Zeevaart, Thomas Ebenhan
Summary: This study demonstrates that a microemulsion can effectively deliver traces of [Ga-68]Ga-PSMA-617 intravenously without losing its properties or causing toxicity, and has the capability to alter the distribution of [Ga-68]Ga-PSMA-617 post injection.
Article
Plant Sciences
Molelekwa Arthur Moroole, Simeon Albert Materechera, Wilfred Otang-Mbeng, Rose Hayeshi, Cor Bester, Adeyemi Oladapo Aremu
Summary: This study assessed the chemical composition, toxicity, and efficacy of a herbal mixture used for contraception by the Batswana of South Africa. The results showed that the herbal mixture was safe but had limited contraceptive efficacy at the tested doses.
Editorial Material
Oncology
Tashinga E. Bapiro, Rose Hayeshi, Collet Dandara
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Boitumelo Moetlhoa, Mabotse Tjale, Alri Pretorius, Rose Hayeshi, Anne Grobler, Nobalanda B. Mokoena
Summary: This study evaluated lactose and sucrose-based stabilizers combined with lyophilization to enhance the stability of the RVF Clone 13 vaccine strain. The sucrose-based formulation of the vaccine had a glass transition temperature (Tg) of 97.0 degrees C with residual moisture below 2%. The lactose formulation had a Tg of 83.5 degrees C and residual moisture above 2%. The sucrose-based formulation maintained higher antigen titers during lyophilization compared to the lactose-formulated vaccine. Cellular-mediated and humoral immunity were evaluated and compared for the two newly formulated vaccines. Pheroid (R) technology was also investigated as a potential adjuvant.
Review
Plant Sciences
Brunhilde De Vos, Rose K. Hayeshi, Wihan Pheiffer, Trevor T. Nyakudya, Ashwell R. Ndhlala
Summary: Diabetes mellitus is a prevalent disease globally, particularly challenging for low-income countries. This review examines the anti-hyperglycaemic potential of traditional medicinal plants Catharanthus roseus and Portulacaria afra in the management of diabetes, providing insights for alternative treatments.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)