期刊
BIOORGANIC CHEMISTRY
卷 38, 期 1-3, 页码 120-123出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bioorg.2010.01.003
关键词
1-Deoxy-D-xylulose 5-phosphate; Preparation; Optimization; Methylerythritol phosphate (MEP) pathway
资金
- National Science Foundation of China (NSFC) [20772095]
- Northwest University [09YZZ59]
The preparation of 1-deoxy-D-xylulose 5-phosphate, the key intermediate of MEP biosynthetic pathway for terpenoids by using recombinant 1-deoxy-D-xylulose 5-phosphate synthase of Rhodobacter capsulatus was optimized. The simple one-pot synthesis coupling with a newly established ion-exchange purification process affords the target compound with more than 80% yield and high purity (>95%). The procedure can also be employed to synthesize isotope labeled 1-deoxy-D-xylulose 5-phosphate by using isotope labeled starting materials. (C) 2010 Elsevier Inc. All rights reserved.
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