期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 24, 期 5, 页码 1307-1309出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2014.01.047
关键词
Cofactor; Malic enzyme; Nicotinamide adenine dinucleotide; Orthogonal redox system; Enzyme kinetics
资金
- National Basic Research and Development Program of China [2012CB721103]
- National Natural Science Foundation of China [21102143, 21325627]
An activity screening between 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide (NAD) analogs and malic enzyme (ME) mutants identified some mutants capable of taking NAD analogs as the cofactor. One particular pair, ME-L310K/L404S and the analog B-8 had good catalytic efficiency and cofactor specificity. The new system gained about 1200-fold cofactor specificity shift from NAD toward B-8 in terms of oxidative decarboxylation of L-malate. Our results provided insightful information for the development of orthogonal redox system that is of particular important to precisely control engineered metabolic pathways. (C) 2014 Elsevier Ltd. All rights reserved.
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