期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 23, 页码 12663-12670出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b03381
关键词
aptasensor; Eu-MWCNTs; electrochemiluminescence; target recycling; thrombin
资金
- Natural Science Foundation of China [21405059, 21203109]
- Shandong Province [ZR2014BL024]
- University of Jinan [XKY1405]
- National Science Center [4UMO-2012/07/B/ST4/01400]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
- University of Jinan
A novel electrochemiluminescent (ECL) aptasensor was proposed for the determination of thrombin (TB) using exonuclease-catalyzed target recycling and hybridization chain reaction (HCR) to amplify the signal. The capture probe was immobilized on an Au-GS-modified electrode through a Au-S bond. Subsequently, the hybrid between the capture probe and the complementary thrombin binding aptamer (TBA) was aimed at obtaining double-stranded DNA (dsDNA). The interaction between TB and its aptamer led to the dissociation of dsDNA because TB has a higher affinity to TBA than the complementary strands. In the presence of exonuclease, aptamer was selectively digested and TB could be released for target recycling. Extended dsDNA was formed through HCR of the capture probe and two hairpin DNA strands (NH2-DNA1 and NH2-DNA1). Then, numerous europium multiwalled carbon nanotubes (Eu-MWCNTs) could be introduced through amidation reaction between NH2-terminated DNA strands and carboxyl groups on the Eu-MWCNTs, resulting in an increased ECL signal. The multiple amplification strategies, including the amplification of analyte recycling and HCR, and high ECL efficiency of Eu-MWCNTs lead to a wide linear range (1.0 X 10(-12)-5.0 x 10(-9) mol/L) and a low detection limit (0.23 pmol/L). The method was applied to serum sample analysis with satisfactory results.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据