Protective effects of a benzoxazine derivative against oxidized LDL-induced apoptosis and the increases of integrin β4, ROS, NF-κB and P53 in human umbilical vein endothelial cells

To investigate whether 6-amino-2,3-dihydro-3-hydroxymethyl-1,4-benzoxazine (ABO) inhibits oxidized low-density lipoprotein (oxLDL)-induced human umbilical vein endothelial cell (HUVEC) apoptosis, we treated HUVECs with oxLDL in the absence or presence of ABO. The results showed that ABO could act as an effective inhibitor of oxLDL-elicited HUVEC apoptosis by inhibiting the levels of integrin beta 4, reactive oxygen species (ROS), NF-kappa B and P53, and suppressing NF-kappa B nuclear translocation. (C) 2009 Elsevier Ltd. All rights reserved.