Article
Chemistry, Applied
Willmann Antonio Jimenez-Morales, Maria del Pilar Canizares-Macias, Jose Pedraza-Chaverri
Summary: A novel fluorescent ORAC-SIA method was developed to determine antioxidant capacity in food samples. The method showed improved efficiency and precision compared to the standard micmplate method.
Article
Food Science & Technology
Juan David Figueroa, Noreima Barroso-Torres, Marcela Morales, Barbara Herrera, Mario Aranda, Eva Dorta, Camilo Lopez-Alarcon
Summary: The ORAC assay does not differentiate between peptides based on their chemical structures and the contribution of oxidation products. Peptides showed similar ORAC values, but the bond dissociation energy (BDE) varied.
Article
Biochemistry & Molecular Biology
Sung Ho Lim, Chang-Ik Choi
Summary: Oxidative stress is linked to various diseases, and studies have shown that raspberry ketone exhibits antioxidant properties by increasing total antioxidant capacity, upregulating antioxidant enzymes, and improving lipid peroxidation. Further clinical studies are needed to verify its value as an effective antioxidant and therapeutic agent.
Article
Environmental Sciences
Juliana da Silva Fonseca, Miguel Mies, Alana Paranhos, Satie Taniguchi, Arthur Z. Guth, Marcia C. Bicego, Joseane Aparecida Marques, Laura Fernandes de Barros Marangoni, Adalto Bianchini
Summary: This study investigated the combined effects of thermal stress and copper exposure on the trophic behavior and oxidative status of the reef-building coral Mussismilia harttii. The findings showed that the trophic behavior remained mostly autotrophic under individual and combined stressors, while the 12-day experiment revealed changes in heterotrophy markers. Additionally, different responses were observed in LPO and TAC levels for both coral and symbiont under the combined stressors, highlighting the differential physiological responses of host and symbionts to global and local stressors.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Nayely Leyva-Lopez, Melissa Peraza-Arias, Anaguiven Avalos-Soriano, Crisantema Hernandez, Cynthia E. Lizarraga-Velazquez, J. Basilio Heredia
Summary: Polyphenolic extracts from spent coffee grounds exhibit antioxidative protective effects against oxidative stress in C. viridis brain cells by enhancing antioxidant enzyme activity and reducing lipid peroxidation.
Article
Fisheries
Oleksandr Malinovskyi, Samad Rahimnejad, Vlastimil Stejskal, Dominik Bonko, Alzbeta Stara, Josef Velisek, Tomas Policar
Summary: This study revealed that photoperiod duration significantly affects the growth rate and antioxidant capacity of largemouth bass. The 8L:16D group showed the best performance in terms of growth, organosomatic index, and antioxidant capacity. Longer light duration led to oxidative stress in fish liver, brain, and gill, but also boosted antioxidant responses in liver, brain, and kidney.
Article
Biochemistry & Molecular Biology
Azza A. A. Attia, Jehan M. M. Sorour, Neama A. A. Mohamed, Tagreed T. T. Mansour, Rasha A. A. Al-Eisa, Nahla S. S. El-Shenawy
Summary: This study aimed to investigate the protective role of vitamin E in improving cardiotoxicity induced by cyclophosphamide in rats. The results showed that vitamin E could attenuate the elevation of cardiac markers, increase in lipid profiles, reduction in antioxidant enzyme activities, and increase in lipid peroxidation. In addition, vitamin E was found to improve the histopathological alterations caused by cyclophosphamide.
Article
Biochemistry & Molecular Biology
Sabina Galiniak, Mateusz Molon, Marta Rachel
Summary: Cystic fibrosis patients experience significant oxidative stress, which increases with disease severity and varies according to the type of bacterial infection.
Article
Fisheries
Liliane S. Presa, Gabriel C. Neves, Lucas C. Maltez, Luis A. Sampaio, Jose M. Monserrat, Carlos E. Copatti, Luciano Garcia
Summary: This study evaluated the effects of nitrate on juvenile mullet in freshwater and found that exposure to higher concentrations of nitrate impacted the physiological homeostasis of mullets, but did not cause lipid peroxidation.
AQUACULTURE RESEARCH
(2022)
Article
Marine & Freshwater Biology
Priscila Rodrigues Siqueira, Jaqueline Perola Souza, Francine Perri Venturini, Talita Laurie Lustosa Carmo, Vinicius Cavicchioli Azevedo, Bianca Martins Estevao, Marina Marques Bonomo, Fabricio Aparecido Santos, Valtencir Zucolotto, Marisa Narciso Fernandes
Summary: Graphene oxide (GO) and reduced graphene oxide (rGO) have wide applications and their waste production leads to contamination in water. Little is known about their interaction with fish cells. In this study, we investigated the effects of GO and rGO exposure on zebrafish liver cells. GO exposure increased antioxidant activity and protected the cells from oxidative damage, while rGO exposure resulted in increased ROS production, lipid peroxidation, and DNA damage. rGO was more toxic to the cells than GO, even at low concentrations.
AQUATIC TOXICOLOGY
(2023)
Article
Plant Sciences
Mustapha Tacherfiout, Sarah Kherbachi, Meriem Kheniche, Marco Mattonai, Ilaria Degano, Erika Ribechini, Bachra Khettal
Summary: This study aimed to investigate the phenolic composition, antioxidant, and antihemolytic effects of hydroalcoholic extracts of Chamaemelum nobile (CN) and Mentha pulegium (MP). The results showed that both extracts exhibited good antioxidant activity and significantly reduced oxidative damage in human erythrocytes induced by AAPH.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Czlapka-Matyasik, Anna Gramza-Michalowska
Summary: This study analyzed the total dietary antioxidant capacity (TAOX), seasonal variability, and sources in the daily diets of cardiovascular disease (CVD) patients. The primary sources of antioxidants in CVD patients' diets were drinks, fruits, vegetables, and black tea. TAOX significantly depended on the season, with the highest in summer and lowest in spring. This suggests that the antioxidant capacity of CVD patients' diets may fluctuate between seasons, and dietary recommendations should be modified accordingly.
Article
Chemistry, Multidisciplinary
Shuhei Sakurai, Akito Kikuchi, Hiroaki Gotoh
Summary: This study evaluated the antioxidant capacities of natural phenols and their derivatives using the H-ORAC method and constructed a database. Although no macroscopic correlation was observed for the antioxidant capacity expression, a difference in the trend of H-ORAC values for each functional group was observed.
Article
Biodiversity Conservation
Sarita Nunes Loureiro, Lilian Lund Amado, Tommaso Giarrizzo
Summary: Aquatic ecosystems are often exposed to complex mixtures of pollutants, which can negatively impact resident organisms. This study evaluated two fish species as potential biomonitors for Amazonian rivers. The results showed species-specific and tissue-specific responses to pollution, with detritivorous species exhibiting more significant biomarker responses and oxidative damage compared to the apex predator. The findings suggest that Prochilodus nigricans is the more sensitive and suitable biomonitor for environmental monitoring. The study also highlights the need for an integrated approach to assess biochemical biomarker responses, including the analysis of different tissue types.
ECOLOGICAL INDICATORS
(2023)
Article
Food Science & Technology
Phung Diep Huy Vu, Akkaratch Rodklongtan, Pakamon Chitprasert
Summary: The study found that the encapsulant weight ratio of whey protein isolate-lignin complex significantly affects the survival rate of spray-dried probiotics, with the 9:1 ratio offering the best protection. Lignin improves the survival of probiotics by enhancing the structure and antioxidant properties of WPI.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aya Umeno, Masaki Tanito, Sachiko Kaidzu, Yasuyuki Takai, Yasukazu Yoshida
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Pharmacology & Pharmacy
Takuya Imatoh, Kimie Sai, Yoshiro Saito
JOURNAL OF CLINICAL PHARMACOLOGY
(2020)
Article
Environmental Sciences
Naoki Endo, Takashi Toyama, Akira Naganuma, Yoshiro Saito, Gi-Wook Hwang
Meeting Abstract
Biochemistry & Molecular Biology
Yoshiro Saito
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yoshiro Saito
Summary: Lipid peroxidation and its products play a significant role in physiological and pathophysiological conditions, with mechanisms including enzymatic oxidation, free radical-mediated oxidation, and nonradical-mediated oxidation. The products of lipid peroxidation can lead to cytotoxicity and novel biological activity, while the antioxidant system, particularly selenoproteins and vitamin E, plays a crucial role in preventing lipid peroxidation and ferroptosis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Multidisciplinary Sciences
Takashi Toyama, Takayuki Hoshi, Takuya Noguchi, Yoshiro Saito, Atsushi Matsuzawa, Akira Naganuma, Gi-Wook Hwang
Summary: Methylmercury-induced neuronal cell death in the mouse brain may be associated with activation of the ASK1/p38 pathway by mitochondrial ROS, leading to TNF-alpha expression and microglia involvement.
SCIENTIFIC REPORTS
(2021)
Review
Nutrition & Dietetics
Yoshiro Saito
Summary: SELENOP is a selenium-containing protein primarily produced in the liver, playing a pivotal role in selenium metabolism and antioxidative defense. It can reduce phospholipid hydroperoxides and deliver selenium to other tissues, but decreased or excessive levels can cause various dysfunctions. This review focuses on SELENOP's selenium transport system, molecular mechanism, and its biological significance.
FRONTIERS IN NUTRITION
(2021)
Article
Chemistry, Analytical
Yeji Kim, Yuuki Inoue, Hijiri Hasegawa, Yasukazu Yoshida, Toshiya Sakata
Summary: This study introduces a novel method for detecting DNA methylation through conformational transition to G-quadruplex, using a solution-gated field-effect transistor (SG-FET) without the need for labeled materials. The method involves grafting a dense hydrophilic polymer brush onto the SG-FET sensor surface to reduce electrical noise and achieve high signal-to-noise ratio analysis of DNA sequences.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yoshiro Saito
Summary: Vitamin E is a potent lipid-soluble antioxidant, consisting of tocopherol and tocotrienol. They have different isoforms which exhibit similar radical scavenging abilities, but offer unique cytoprotective effects in cells. The review focuses on the actions of vitamin E against oxidative stress and its relation to cellular uptake and distribution, as well as its role in preventing lipid peroxidation and ferroptosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Machiko Honda, Tatsuya Segawa, Kiyoshi Ishikawa, Masahiro Maeda, Yoshiro Saito, Shigeyuki Kon
Summary: Blood levels of nephronectin (Npnt), a protein critical for kidney development, are elevated in EAE mice. Treatment with anti-Npnt antibody inhibits EAE development. The interaction between Npnt and SeP regulates the Th17/Treg balance via the ROS level.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nanako Kitabayashi, Shohei Nakao, Yuichiro Mita, Kotoko Arisawa, Takayuki Hoshi, Kiyo-aki Ishii, Toshinari Takamura, Noriko Noguchi, Yoshiro Saito, Takashi Toyama
Summary: Selenoprotein P (SELENOP) is a selenium-containing protein that plays a significant role in pancreatic beta cell function by maintaining the levels of cellular selenoproteins. Knockdown of the Selenop gene resulted in decreased cell viability, insulin levels, and insulin secretion, which were recovered by the addition of selenium. These findings suggest that SELENOP expression is important for the function of pancreatic beta cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Allergy
Hirohisa Ogawa, Masahiko Azuma, Aya Umeno, Mayuko Shimizu, Kazutoshi Murotomi, Yasukazu Yoshida, Yasuhiko Nishioka, Koichi Tsuneyama
Summary: In this study, the roles of neutrophils and nerve growth factor (NGF) in refractory asthma were examined. The results showed that neutrophil myeloperoxidase (MPO)-derived singlet oxygen induces increased NGF production, leading to airway hyperreactivity (AHR) and the production of specific markers. These findings contribute to the development of new therapies and biomarkers for non-type 2 and refractory asthma.
ALLERGOLOGY INTERNATIONAL
(2022)
Review
Environmental Sciences
Marco Vinceti, Tommaso Filippini, Ewa Jablonska, Yoshiro Saito, Lauren A. Wise
Summary: Recent evidence suggests that previous research underestimated the adverse effects of selenium exposure at low levels and the potential drawbacks of maximizing selenoprotein expression. This calls for a reassessment of the recommended range of selenium exposure and dietary reference values.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Teresa Urbano, Marco Vinceti, Jessica Mandrioli, Annalisa Chiari, Tommaso Filippini, Roberta Bedin, Manuela Tondelli, Cecilia Simonini, Giovanna Zamboni, Misaki Shimizu, Yoshiro Saito
Summary: This study found abnormalities in the concentrations of selenium-transporter protein selenoprotein P in the central nervous system and blood, which are associated with amyotrophic lateral sclerosis (ALS) and neurocognitive disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Takayuki Kaneko, Yuichiro Mita, Kanako Nozawa-Kumada, Masana Yazaki, Mieko Arisawa, Etsuo Niki, Noriko Noguchi, Yoshiro Saito
Summary: This study reveals the role of hydrogen sulfide, hydropersulfides, and hydropolysulfides in cell signaling and protection against oxidative stress. The mechanism of action involves both two-electron and one-electron redox reactions, with the former receiving more attention. The study also highlights the antioxidant effects of these compounds in inhibiting lipid peroxidation.
FREE RADICAL RESEARCH
(2022)
Article
Chemistry, Medicinal
Shibin Zhao, Julian Maceren, Mia Chung, Samantha Stone, Raphael Geiben, Melissa L. Boby, Bradley S. Sherborne, Derek S. Tan
Summary: Antibiotic resistance is a major threat to public health, with Gram-negative bacteria presenting unique challenges due to their low permeability and efflux pumps. Limited understanding of the chemical rules for overcoming these barriers hinders antibacterial drug discovery. Efforts to address this issue, such as screening compound libraries and using cheminformatic analysis, have led to the design of sulfamidoadenosines with diverse substituents, showing potential utility in accumulation in Escherichia coli.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Jichun Li, Qing Li, Shuai Xia, Jiahuang Tu, Longbo Zheng, Qian Wang, Shibo Jiang, Chao Wang
Summary: This study successfully developed a short peptide mimetic as a MERS-CoV fusion inhibitor by reproducing the key recognition features of the HR2 helix. The resulting 23-mer lipopeptide showed comparable inhibitory effect to the 36-mer HR2 peptide HR2P-M2. This has important implications for developing short peptide-based antiviral agents to treat MERS-CoV infection.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Krista Jaunsleine, Linda Supe, Jana Spura, Sten van Beek, Anna Sandstrom, Jessica Olsen, Carina Halleskog, Tore Bengtsson, Ilga Mutule, Benjamin Pelcman
Summary: Beta(2)-adrenergic receptor agonists can stimulate glucose uptake by skeletal muscle cells and are therefore potential treatments for type 2 diabetes. The chirality of compounds has a significant impact on the activity of these agonists. This study found that certain synthesized compounds showed higher glucose uptake activity. These findings provide important information for the design of novel beta(2)AR agonists for T2D treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Xin Xu, Jia Chen, Guan Wang, Xiaojuan Zhang, Qiang Li, Xiaobo Zhou, Fengying Guo, Min Li
Summary: The study focuses on EZH2, a promising therapeutic target for various types of cancers. Researchers designed and synthesized a series of novel derivatives aiming to enhance the EZH2 inhibition activity. Among them, compound 28 displayed potent EZH2 inhibition activity and showed high anti-proliferative effects in lymphoma cell lines and xenograft mouse models. The study suggests that compound 28 has potential as a therapeutic candidate for EZH2-associated cancers.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Wei Zhang, Wei Liu, Ya-Dong Zhao, Li-Zi Xing, Ji Xu, Rui-Jun Li, Yun-Xiao Zhang
Summary: This study developed a series of aromatic amide derivatives based on Rhein and investigated their inhibitory activity against alpha-Syn aggregation. Two of these compounds showed promising potential in treating Parkinson's disease by stabilizing alpha-Syn's conformation and disassembling alpha-Syn oligomers and fibrils.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Mani Sharma, S. S. S. S. Sudha Ambadipudi, Neeraj Kumar Chouhan, V. Lakshma Nayak, Srihari Pabbaraja, Sai Balaji Andugulapati, Ramakrishna Sistla
Summary: Therapeutically active lipids in drug delivery systems can enhance the safety and efficacy of treatment. The liposome formulation created using synthesized biologically active lipids showed additive anti-cancer effects and reduced tumorigenic potential.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
