Article
Biochemistry & Molecular Biology
Ioana Maria Carmen Ienascu, Adina Cata, Mariana Nela Stefanut, Iuliana Popescu, Gerlinde Rusu, Paula Sfirloaga, Daniel Ursu, Cristina Mosoarca, Anamaria Dabici, Corina Danciu, Delia Muntean, Raluca Pop
Summary: The goal of this research was to design novel chloro-substituted salicylanilide derivatives and their beta-cyclodextrin complexes in order to obtain efficient antibacterial compounds and to demonstrate the beneficial role of complexation on the efficiency of these compounds.
Article
Biochemistry & Molecular Biology
Hao-Chun Chuang, Ming-Fang Liu, Hsin-Yi Wu, Ying-Ta Wu, Ting-Jen Rachel Cheng, Jim-Min Fang
Summary: A benzophenone-containing salicylanilide compound was synthesized and used as a photoaffinity probe to identify the binding site of Acinetobacter baumannii penicillin-binding protein. Molecular docking experiments suggested that the binding site is a hydrophobic pocket that can disrupt the growth of peptidoglycan chain.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Dodda Bhargavi, Srihari Konduri, Jyothi Prashanth, Sowjanya Pulipati, K. K. Praneeth, Malladi Sireesha, Koya Prabhakara Rao
Summary: We discovered twenty-two new sacubitril derivatives (5a-v) that can serve as lead compounds for various biologically active targets. These compounds were synthesized by reacting an intermediate compound with respective carboxylic acid. The molecular structures of these compounds were determined using different analytical techniques, and their biological activities were evaluated, including antifungal, antibacterial, and anti-TB properties.
Article
Biochemistry & Molecular Biology
Jacqueline V. Lara-Espinosa, Maria Fernanda Arce-Aceves, Manuel O. Lopez-Torres, Vasti Lozano-Ordaz, Dulce Mata-Espinosa, Jorge Barrios-Payan, Carlos Alfredo Silva-Islas, Perla D. Maldonado, Brenda Marquina-Castillo, Rogelio Hernandez-Pando
Summary: Curcumin (CUR), a natural product with antioxidant, anti-inflammatory, and antibacterial activities, has been found to have a bactericidal effect on pulmonary mycobacterial infection and reduce neuroinflammation in a model of progressive pulmonary tuberculosis in mice. This suggests that CUR has potential as an adjuvant therapy in TB treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Wassihun Wedajo Aragaw, Brendon M. Lee, Xuan Yang, Matthew D. Zimmerman, Martin Gengenbacher, Wai-Keung Chui, Colin J. Jackson, Thomas Dick
Summary: The tuberculosis drug TA-C is metabolized by mycobacterial oxidoreductases to produce a more potent DHFR inhibitor than the parent compound, explaining the discrepancy between enzymatic and whole-cell activity observed in previous studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Xiaohong Yue, Xiangsheng Ke, Yafei Shi, Yangsheng Li, Chenhao Zhang, Yetao Wang, Xin Hou
Summary: The maintenance of a proton gradient across the thylakoid membrane is crucial for photosynthesis, and disruption of this process can occur under high temperatures. In this study, a heat-sensitive mutant was identified, and the mutation was found in the AtFtsH11 gene. AtFtsH11 plays a critical role in degrading the ATP synthase assembly factor BFA3 under heat stress, limiting the amount of ATP synthase integrated into the thylakoid membrane and regulating proton efflux. This study highlights the importance of AtFtsH11 in stabilizing photosynthesis and provides insights into its specific molecular functions.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Victor Kesternich, Marcia Perez-Fehrmann, Victor Quezada, Marina Castroagudin, Ronald Nelson, Rolando Martinez
Summary: This paper presents a method for synthesizing rafoxanide 6, a halogenated salicylanilide used as an efficient anthelmintic in sheep and cattle. The synthesis involves three steps starting from readily available 4-chlorophenol, with an overall yield of 74%. The key stages of the synthesis are salicylic acid iodination achieving a yield of 95% and the reaction of 3,5-diiodosalicylic acid 5 with aminoether 4, resulting in a yield of 82%. The intermediates and final product were characterized using physical and spectroscopic techniques.
Article
Infectious Diseases
Hua Xiao, Xia Yu, Yuanyuan Shang, Ruyan Ren, Yi Xue, Lingling Dong, Liping Zhao, Guanglu Jiang, Hairong Huang
Summary: This study evaluated the in vitro activity of WX-081 against clinical isolates of drug-resistant Mycobacterium tuberculosis (Mtb) and its intracellular bactericidal activity against a reference strain. WX-081 showed potent efficacy against the tested isolates, with 95.6% of strains having a minimum inhibitory concentration (MIC) of ≤0.25 μg/mL. The intracellular killing effect of WX-081 supports its potential efficacy in treating TB patients.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biochemistry & Molecular Biology
Maria Patron, Daryna Tarasenko, Hendrik Nolte, Lara Kroczek, Mausumi Ghosh, Yohsuke Ohba, Yvonne Lasarzewski, Zeinab Alsadat Ahmadi, Alfredo Cabrera-Orefice, Akinori Eyiama, Tim Kellermann, Elena Rugarli, Ulrich Brandt, Michael Meinecke, Thomas Langer
Summary: Mitochondrial proteases, specifically the m-AAA protease AFG3L2, are regulated by the mitochondrial proton gradient and play a crucial role in reshaping the mitochondrial proteome in response to different energetic demands. TMBIM5, a Ca2+/H+ exchanger in the mitochondrial inner membrane, inhibits the activity of the m-AAA protease and ensures cell survival and respiration. However, persistent hyperpolarization triggers the degradation of TMBIM5 and activation of the m-AAA protease, which facilitates the proteolytic breakdown of respiratory complex I to limit ROS production in hyperpolarized mitochondria.
Article
Biology
Benjamin Gottschalk, Zhanat Koshenov, Markus Waldeck-Weiermair, Snjezana Radulovic, Furkan E. Oflaz, Martin Hirtl, Olaf A. Bachkoenig, Gerd Leitinger, Roland Malli, Wolfgang F. Graier
Summary: This study reveals the role of MICU1 in controlling the spatial membrane potential gradients in mitochondria, with changes in SMPGs and spatial cristae depolarization occurring when Ca2+ binds to MICU1.
COMMUNICATIONS BIOLOGY
(2022)
Article
Materials Science, Composites
Fang Chen, Wenjie Dong, Feng Lin, Wanjun Ren, Xiaoyan Ma
Summary: This study balanced the trade-off effect between proton conductivity and swelling ratio of SPAES based PEMs by fabricating SPAES composite membranes with gradient-distribution of POSS nanospheres. The composite membranes exhibited extremely low swelling ratio and maintained high proton conductivity, providing a new approach for fabricating large-size composite membranes for PEMFCs and serving as a reference for other functional membranes related researches such as batteries and filtrations.
COMPOSITES COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Fuhang Song, Jiansen Hu, Xinwan Zhang, Wei Xu, Jinpeng Yang, Shaoyong Li, Xiuli Xu
Summary: Two new cyclized thiolopyrrolone derivatives and one known compound were identified from a marine-derived Streptomyces sp. They exhibited antibacterial activities against different bacteria with varying minimum inhibitory concentrations.
Article
Biochemistry & Molecular Biology
Aisha Alnami, Raymond S. Norton, Helena Perez Pena, Shozeb Haider, Frank Kozielski
Summary: Mycobacterium tuberculosis's FtsZ protein is a potential antibacterial target, with inhibition leading to cell division arrest and eventual cell death. Research has found that a coumarin analogue can bind to novel cryptic pockets in FtsZ, revealing the molecular mechanism behind its antibacterial activity.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Physical
Hua Fang, Honghui Guo, Hui Chen, Jianyu Zhang, Zhuan Hong, Meijuan Fang
Summary: Novel salicylanilide analogues were synthesized and evaluated for their inhibitory activity on polyphenoloxidase (PPO) from Chinese white shrimp. Compound 4q showed the most potent PPO inhibitory activity and could potentially be applied as an anti-browning and antimicrobial agent in the food industry.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Materials Science, Multidisciplinary
Qin Liu, Yong-Xiang Li, Jing Qiu, Mian Chen, Jie Wang, Jian Hu
Summary: In this study, a silvered gradient nanostructured (GNS) layer was fabricated on Ti6Al4V surface by SURT combined with silvering process, showing excellent antibacterial, wear and corrosion resistance properties without cytotoxicity, indicating good bioadaptability.
Article
Infectious Diseases
Sean Wasserman, Gail Louw, Limpho Ramangoaela, Garrick Barber, Cindy Hayes, Shaheed Vally Omar, Gary Maartens, Clifton Barry, Taeksun Song, Graeme Meintjes
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Medicine, General & Internal
Natasha Strydom, Sneha Gupta, William S. Fox, Laura E. Via, Hyeeun Bang, Myungsun Lee, Seokyong Eum, TaeSun Shim, Clifton E. Barry, Matthew Zimmerman, Veronique Dartois, Radojka M. Savic
Article
Multidisciplinary Sciences
Deivide Oliveira-de-Souza, Caian L. Vinhaes, Maria B. Arriaga, Nathella Pavan Kumar, Juan M. Cubillos-Angulo, Ruiru Shi, Wang Wei, Xing Yuan, Guolong Zhang, Ying Cai, Clifton E. Barry, Laura E. Via, Alan Sher, Subash Babu, Katrin D. Mayer-Barber, Helder I. Nakaya, Kiyoshi F. Fukutani, Bruno B. Andrade
SCIENTIFIC REPORTS
(2019)
Article
Microbiology
Yumi Park, Yong-Mo Ahn, Surendranadha Jonnala, Sangmi Oh, Julia M. Fisher, Michael B. Goodwin, Thomas R. Ioerger, Laura E. Via, Tracy Bayliss, Simon R. Green, Peter C. Ray, Paul G. Wyatt, Clifton E. Barry, Helena Boshoff
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Chemistry, Medicinal
Andrew J. Whitehouse, M. Daben J. Libardo, Monica Kasbekar, Paul D. Brear, Gerhard Fischer, Craig J. Thomas, Clifton E. Barry, Helena I. M. Boshoff, Anthony G. Coyne, Chris Abell
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Tiago Beites, Kathryn O'Brien, Divya Tiwari, Curtis A. Engelhart, Shaun Walters, Jenna Andrews, Hee-Jeong Yang, Michelle L. Sutphen, Danielle M. Weiner, Emmanuel K. Dayao, Matthew Zimmerman, Brendan Prideaux, Prashant V. Desai, Thierry Masquelin, Laura E. Via, Veronique Dartois, Helena I. Boshoff, Clifton E. Barry, Sabine Ehrt, Dirk Schnappinger
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Qinglan Wang, Helena I. M. Boshoff, Justin R. Harrison, Peter C. Ray, Simon R. Green, Paul G. Wyatt, Clifton E. Barry
Review
Medicine, General & Internal
Anthony Lee, Yingda Linda Xie, Clifton E. Barry, Ray Y. Chen
BMJ-BRITISH MEDICAL JOURNAL
(2020)
Editorial Material
Chemistry, Medicinal
Manu De Rycker, David Horn, Bree Aldridge, Richard K. Amewu, Clifton E. Barry, Frederick S. Buckner, Sarah Cook, Michael A. J. Ferguson, Nathalie Gobeau, Jennifer Herrmann, Paul Herding, William Hope, Jennifer Keiser, Maria Jose Lafuente-Monasterio, Paul D. Leeson, Didier Leroy, Ujjini H. Manjunatha, James McCarthy, Timothy J. Miles, Valerie Mizrahi, Olena Moshynets, Jacquin Niles, John P. Overington, John Pottage, Srinivasa P. S. Rao, Kevin D. Read, Isabela Ribeiro, Lynn L. Silver, Jen Southern, Thomas Spangenberg, Shyam Sundar, Caitlin Taylor, Wes Van Voorhis, Nicholas J. White, Susan Wyllie, Paul G. Wyatt, Ian H. Gilbert
ACS INFECTIOUS DISEASES
(2020)
Article
Radiology, Nuclear Medicine & Medical Imaging
Stephanus T. Malherbe, Ray Y. Chen, Patrick Dupont, Ilse Kant, Magdalena Kriel, Andre G. Loxton, Bronwyn Smith, Caroline G. G. Beltran, Susan van Zyl, Shirely McAnda, Charmaine Abrahams, Elizna Maasdorp, Alex Doruyter, Laura E. Via, Clifton E. Barry, David Alland, Stephanie Griffith Richards, Annare Ellman, Thomas Peppard, John Belisle, Gerard Tromp, Katharina Ronacher, James M. Warwick, Jill Winter, Gerhard Walzl
Article
Immunology
Shunsuke Sakai, Keith D. Kauffman, Sangmi Oh, Christine E. Nelson, Clifton E. Barry, Daniel L. Barber
Summary: The study found that MAIT cells mount minimal responses following Mtb infection, but MAIT cell expansion during chronic infection can reduce bacterial loads. The 5-OP-RU vaccination failed to protect against Mtb challenge, but MAIT cells play a role in delaying T cell priming.
MUCOSAL IMMUNOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Bree B. Aldridge, David Barros-Aguirre, Clifton E. Barry, Robert H. Bates, Steven J. Berthel, Helena I. Boshoff, Kelly Chibale, Xin-Jie Chu, Christopher B. Cooper, Veronique Dartois, Ken Duncan, Nader Fotouhi, Fabian Gusovsky, Philip A. Hipskind, Dale J. Kempf, Joel Lelievre, Anne J. Lenaerts, Case W. McNamara, Valerie Mizrahi, Carl Nathan, David B. Olsen, Tanya Parish, H. Michael Petrassi, Alexander Pym, Kyu Y. Rhee, Gregory T. Robertson, Jeremy Michael Rock, Eric J. Rubin, Betsy Russell, David G. Russell, James C. Sacchettini, Dirk Schnappinger, Michael Schrimpf, Anna M. Upton, Peter Warner, Paul Graham Wyatt, Ying Yuan
Summary: The Tuberculosis Drug Accelerator has successfully facilitated collaboration in tuberculosis drug discovery, breaking down barriers among competing labs and institutions. The achievements, advantages, and limitations of the consortium suggest that similar models could be applied to research on other diseases.
Editorial Material
Immunology
Clifton E. Barry, Katrin D. Mayer-Barber
Summary: Despite the majority of humans infected with Mycobacterium tuberculosis not showing clinical symptoms, predicting those who will remains challenging. Recent studies have revealed patterns and pathways that determine who is at highest risk for progression.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Editorial Material
Critical Care Medicine
Christoph Lange, Clifton E. Barry, C. Robert Horsburgh
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Biology
Kira S. Makarova, Brittney Blackburne, Yuri Wolf, Anastasia Nikolskaya, Svetlana Karamycheva, Marlene Espinoza, Clifton E. Barry, Carole A. Bewley, Eugene Koonin
Summary: This study comprehensively analyzed BGCs encoding graspetides and reported several novel graspetide groups and associated proteins involved in their biosynthesis and expression. The results revealed previously unsuspected diversity in graspetide sequences and expanded the known diversity of RiPPs. The findings of this study are important for advancing approaches to identify RiPPs.