Article
Agricultural Engineering
Zhuoyu Zhou, Jie Shen, Qinbing Guo, Yongmei Xia, Xueyi Hu, Xiang Liu, Jing Wu
Summary: This study investigated the enzymatic production pathway of rubusoside from stevioside, the performance of rubusoside in solubilization of vitamins, and the identification of byproducts. Rubusoside significantly enhanced the solubility of Vitamin E and Vitamin D2, showing promise for potential applications beyond just being a sweetener.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Food Science & Technology
Anuchita Moongngarm, Naruetit Sriharboot, Patiwit Loypimai, Tanongsak Moontree
Summary: The study investigated the effect of ohmic heating-assisted water extraction (OH-AWE) on the extraction of steviol glycosides and phytochemicals from Stevia rebaudiana leaves. Results showed that OH-AWE resulted in higher stevioside and rebaudioside A content, as well as total flavonoids compared to untreated leaves and conventional methods. The optimal extraction conditions were found to be 30% and 40%MC leaves under OH-AWE, resulting in the highest chlorogenic, syringic, caffeic, and ferulic acids, as well as total phenolic contents. OH-AWE also provided the highest concentration of steviol glycosides in extract powder and optimal extraction yields.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Jung-Min Park, Jung-Hoon Lee, Jong-Ho Koh, Jin-Man Kim
Summary: This study developed a method to better separate and quantify steviol glycosides in various foods, with validated accuracy and precision.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
T. P. Magangana, M. A. Stander, N. A. Masondo, N. P. Makunga
Summary: Plants exhibit phenotypic plasticity under different environmental conditions, with the ability to produce more secondary metabolites when microenvironmental conditions are manipulated. However, responses may be dependent on species, cultivar, and genotype. In this study, in vitro plants of S. rebaudiana Bertoni were subjected to salt and drought stress, resulting in poor growth and development and reduced levels of key steviol glycosides. Additionally, the essential oil chemistry of the plants was significantly altered under stress conditions, highlighting the potential negative impact of environmental stresses on the bioactive aromatic compounds of the plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biochemistry & Molecular Biology
Liangliang Chen, Huayi Pan, Ruxin Cai, Yan Li, Honghua Jia, Kequan Chen, Ming Yan, Pingkai Ouyang
Summary: In this study, new steviol glycosides were enzymatically synthesized from stevioside by using plant enzymes, with rebaudioside E as the main product. The regioselectivity and stereoselectivity of the enzymes were validated, leading to the successful production of a high-intensity sweetener.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alexandra D. Voloshina, Anastasiia S. Sapunova, Natalia V. Kulik, Mayya G. Belenok, Irina Yu Strobykina, Anna P. Lyubina, Syumbelya K. Gumerova, Vladimir E. Kataev
Summary: The study found that several ammonium derivatives of diterpenoids steviol and isosteviol exhibited high antibacterial activity against MRSA strains and cytotoxic effects on cancer cell lines. It also showed potential anticancer effects on MCF-7 cells while causing minimal harm to normal cells, indicating promising scaffolds for the development of new antibacterial drugs and anticancer agents targeting mitochondria.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Plant Sciences
Hojjat Baroni-nezhad, Mojtaba Karimi, Hamidreza Motaghian, Fatemeh Direkvand-moghadam
Summary: Micronutrients like Copper, Iron, and Zinc do not significantly affect the growth of Stevia, but can markedly increase the content of sweet compounds such as steviol glycosides in the leaves.
JOURNAL OF PLANT NUTRITION
(2021)
Article
Food Science & Technology
Jakub Michal Kurek, Joanna Zielinska-Wasielica, Katarzyna Kowalska, Zbigniew Krejpcio, Anna Olejnik
Summary: This study demonstrates the inhibitory effect of stevioside and steviol on adipogenesis and lipogenesis, leading to decreased lipid accumulation. Additionally, they can enhance glucose uptake in insulin-resistant cells and alleviate symptoms of insulin resistance.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biotechnology & Applied Microbiology
Xueyi Hu, Zhuoyu Zhou, Zongying Zhang, Xiaoxia Wang, Xiaochen Sui, Junming Chen, Yongmei Xia, Jue Zhang, Jianguo Lin
Summary: Isosteviol can be efficiently prepared from stevioside using an in-situ separation-coupling-reaction method, which shows promising inhibition effects on tumor cells. Acidic ion-exchange resins demonstrate higher specificity for isosteviol production compared to other acids and ionic liquids.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Microbiology
Zhenxin Yan, Xueting Cao, Xiao Yang, Shida Yang, Li Xu, Xukai Jiang, Min Xiao
Summary: A novel β-glucosidase (CsBGL) was isolated from environmental microorganisms, which efficiently hydrolyzed stevioside to produce rubusoside. This enzyme could be a promising biocatalyst for the industry-scale production of rubusoside from stevioside.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Hui-Ling Jiang, Yan Xuan, Qian Zeng, Qi-Ming Jimmy Yu, Yu-Qian Zhang, Yu-Ru Chen, Hai-Bo Luo, He Huang, Qing Xu
Summary: A strain capable of degrading steviol glycosides to steviol (STE) was isolated from soil samples, identified as Microbacterium barkeri XJ. The bioconversion rates of different steviol glycosides into STE by XJ were found to be more effective than mixed flora. This study highlights the potential of XJ in the bioconversion of steviol glycosides.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2021)
Article
Agronomy
Asish Kumar Ghose, Siti Nor Akmar Abdullah, Muhammad Asyraf Md Hatta, Puteri Edaroyati Megat Wahab
Summary: A plant tissue culture protocol from stevia was optimized for the production of planting materials and rebaudioside A, a natural sweetener. By adjusting the culture medium and supplement concentrations, the survival rate and callus production were improved, resulting in higher yields of shoots and roots. The highest concentration of rebaudioside A was obtained using specific media conditions and correlated with the expression level of a biosynthetic gene.
Article
Pharmacology & Pharmacy
Cheng Chen, Xinyu Na, Lei Wang, Rui Yu
Summary: This study identified stevioside as a potential agent for the treatment of bladder cancer. Stevioside selectively inhibited the viability of bladder cancer cells and induced their intrinsic apoptosis. It also activated specific signaling pathways and caused mitochondrial stress in bladder cancer cells. Furthermore, stevioside inhibited the growth of bladder cancer cells in vivo. Overall, these findings suggest that stevioside could be a promising therapeutic option for bladder cancer.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karel Vives Hernandez, Jordi Moreno-Romero, Martha Hernandez de la Torre, Claudia Perez Manriquez, Darcy Rios Leal, Jaime F. Martinez-Garcia
Summary: The study compared the content of stevioside and rebaudioside A in two commonly used S. rebaudiana cultivars, Morita II and Criolla, finding significant differences between them. Enhanced expression of three genes involved in SG biosynthesis in Morita II may contribute to its higher accumulation of rebaudioside A. Light conditions can affect SG accumulation, with higher light intensity leading to increased expression of biosynthetic genes and higher levels of stevioside and rebaudioside A.
Article
Biochemistry & Molecular Biology
Sonam Kumari, Mohammed Sikander, Shabnam Malik, Manish K. Tripathi, Bilal B. Hafeez, Murali M. Yallapu, Subhash C. Chauhan, Sheema Khan, Meena Jaggi
Summary: Steviol effectively inhibits glucose consumption and metabolism in pancreatic cancer cells, reducing their aggressiveness by inducing apoptosis and cell cycle arrest in the G1/M phase. It also suppresses tumorigenic and metastatic potential by repressing the phosphorylation of mTOR and translation initiation proteins, suggesting potential for development of combination therapeutic strategies for pancreatic cancer treatment.
