Article
Biochemistry & Molecular Biology
Jing-jiao Wang, Cheng-lei Zhang, Xiao-qian Guo, Chang-yi Yang
Summary: This study investigated the effect of 1,25(OH)(2)VitD(3) on osteogenic differentiation of hPDLSCs in an in vitro periodontitis model and its impact on inflammatory status. The results showed that 1,25(OH)(2)VitD(3) can reverse the inhibitory effect of LPS-G on hPDLSCs proliferation and osteogenic differentiation, and also suppress LPS-G-induced upregulation of inflammatory gene expressions, improving the inflammatory status.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Veterinary Sciences
Catherine E. Ruggiero, Robert C. Backus
Summary: The study aimed to investigate the metabolites of vitamin D-2 in cats and compared the efficacy of vitamin D-2 and 25(OH)D-2 for increasing feline vitamin D status. Results showed that supplementation with 25(OH)D-2 effectively raised circulating levels in cat plasma compared to vitamin D-2. The presence of epimeric forms of vitamin D metabolites in circulation indicates a need for further studies on the safety and efficacy of these vitamers in cats.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Kazuki Maekawa, Michiyasu Ishizawa, Takashi Ikawa, Hironao Sajiki, Taro Matsumoto, Hiroaki Tokiwa, Makoto Makishima, Sachiko Yamada
Summary: The active form of vitamin D-3, 1,25-dihydroxyvitamin D-3, regulates calcium homeostasis through activation of the vitamin D receptor (VDR). Vitamin D derivatives with large adamantane (AD) rings at certain positions of the side chain were synthesized to study their VDR agonist and/or antagonist properties. Novel vitamin D derivatives with an alkyl group substituted at position 25 showed selective VDR activity and enhanced osteoblast differentiation compared to the original derivative, ADTK1. These modified derivatives have the potential for targeted therapy with increased stability and tissue selectivity.
Review
Biochemistry & Molecular Biology
Simona Fenizia, Melania Gaggini, Cristina Vassalle
Summary: This review discusses the interaction between vitamin D and sphingolipids, with a focus on cardiometabolic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Pierre Delanaye, Antoine Lanot, Antoine Bouquegneau, Xavier Warling, Luc Radermacher, Catherine Masset, Jean-Marie Krzesinski, Olivier Moranne, Etienne Cavalier
Summary: This study aimed to investigate the necessity of monitoring 1,25-dihydroxyvitamin D levels in hemodialysis patients. The results showed that there was a significant increase in 25-hydroxyvitamin D levels in patients treated with native vitamin D, but no significant change in 1,25-dihydroxyvitamin D levels. For patients treated with active vitamin D, there were no significant changes in both 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D levels. Therefore, monitoring 1,25-dihydroxyvitamin D levels in patients newly treated with active vitamin D does not seem useful.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Danchen Wang, Songlin Yu, Qi Zhang, Qian Cheng, Shaowei Xie, Yuanyuan Zhang, Xiuzhi Guo, Yutong Zou, Yicong Yin, Jialei Yu, Honglei Li, Liangyu Xia, Ling Qiu, Weibo Xia
Summary: This study successfully developed a robust and accurate method for simultaneous measurement of serum 25(OH)D, 1,25(OH)(2)D, and 24,25(OH)(2)D by LC-MS/MS with efficient separation of 3-epi analogs, 23R,25(OH)(2)D-3, and 4 beta,25(OH)(2)D-3.
JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Endocrinology & Metabolism
Chun Zhou, Panpan He, Ziliang Ye, Yuanyuan Zhang, Yanjun Zhang, Sisi Yang, Qimeng Wu, Mengyi Liu, Jing Nie, Xianhui Qin
Summary: The study found an inverse association between serum vitamin D levels and new-onset chronic kidney disease in participants with diabetes. However, this association was not observed in participants without diabetes.
Article
Biochemistry & Molecular Biology
Madeline P. Sheeley, Violet A. Kiesel, Chaylen Andolino, Nadia A. Lanman, Shawn S. Donkin, Stephen D. Hursting, Michael K. Wendt, Dorothy Teegarden
Summary: Metabolic reprogramming plays a key role in cancer progression and metastasis. In this study, the researchers investigated the effects of the bioactive metabolite of vitamin D, 1,25(OH)(2)D, on cell survival in ECM-detached conditions. They found that pretreatment with 1,25(OH)(2)D reduced cell viability in detached conditions and altered glutamine metabolism. Further analysis revealed that 1,25(OH)(2)D suppressed the expression and activity of pyruvate carboxylase (PC), a key enzyme involved in cancer cell anchorage independence. These results suggest that 1,25(OH)(2)D may suppress cancer progression by inhibiting PC and preventing anchorage independence.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Chemistry, Medicinal
Negar Khazan, Kyu Kwang Kim, Jeanne N. Hansen, Niloy A. Singh, Taylor Moore, Cameron W. A. Snyder, Ravina Pandita, Myla Strawderman, Michiko Fujihara, Yuta Takamura, Ye Jian, Nicholas Battaglia, Naohiro Yano, Yuki Teramoto, Leggy A. Arnold, Russell Hopson, Keshav Kishor, Sneha Nayak, Debasmita Ojha, Ashoke Sharon, John M. Ashton, Jian Wang, Michael T. Milano, Hiroshi Miyamoto, David C. Linehan, Scott A. Gerber, Nada Kawar, Ajay P. Singh, Erdem D. Tabdanov, Nikolay Dokholyan, Hiroki Kakuta, Peter W. Jurutka, Nina F. Schor, Rachael B. Rowswell-Turner, Rakesh K. Singh, Richard G. Moore
Summary: This study investigates a novel VDR antagonist MeTC7 that selectively inhibits VDR, suppresses the viability of cancer cell-lines, and reduces the growth of neuroblastoma. The research findings contribute to the development of easy-to-synthesize selective VDR antagonists.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Organic
Jiaming Cheng, Jie Sheng, Xu Cheng
Summary: In this study, an electrochemical deuteration method using deuterium water in DMF was used to convert aromatic trifluoromethyl to methyl-d(3) groups. The addition of a basic additive was crucial for achieving highly selective reaction, resulting in yields up to 94%, D ratio up to 97%, and CD3 incorporation up to 90%.
Article
Chemistry, Organic
Michael Keim, Katharina Konetzke, Angelika Freytag, Gerhard Maas
Summary: 3-(Trifluoromethyl)prop-2-yne 1-iminium triflate salts were synthesized by elimination of triflic acid, and exhibited excellent reactivity in various cycloaddition reactions.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemical Research Methods
Stephen A. Wise, Johanna E. Camara, Carolyn Q. Burdette, Grace Hahm, Federica Nalin, Adam J. Kuszak, Joyce Merkel, Ramon A. Durazo-Arvizu, Emma L. Williams, Andrew N. Hoofnagle, Fiona Ivison, Ralf Fischer, Jody M. W. van den Ouweland, Chung S. Ho, Emmett W. K. Law, Jean-Nicolas Simard, Renaud Gonthier, Brett Holmquist, Sarah Meadows, Lorna Cox, Kimberly Robyak, Michael H. Creer, Robert Fitzgerald, Michael W. Clarke, Norma Breen, Pierre Lukas, Etienne Cavalier, Christopher T. Sempos
Summary: The Vitamin D Standardization Program (VDSP) conducted an interlaboratory comparison study to evaluate the performance of liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays for determining serum total 25-hydroxyvitamin D. Results showed that only 53% of the assays met the VDSP criterion, with four assays having significant performance issues due to the lack of separation between 3-epi-25(OH)D-3 and 25(OH)D-3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
H. R. Neill, C. I. R. Gill, E. J. McDonald, W. C. McRoberts, R. Loy, L. K. Pourshahidi
Summary: The study found that cooking significantly increased the concentration of vitamin D in pork, with different cooking methods having minimal impact on the retention of vitamin D. Interestingly, the type of pork product had a greater influence on the concentration of vitamin D than the cooking method.
Article
Chemistry, Medicinal
Samuel Seoane, Pranjal Gogoi, Araceli Zarate-Ruiz, Carole Peluso-Iltis, Stefan Peters, Thierry Guiberteau, Miguel A. Maestro, Roman Perez-Fernandez, Natacha Rochel, Antonio Mourino
Summary: This article reports the development of two highly active and noncalcemic analogues of 1,25D3 that lack the C-ring and possess an m-phenylene ring that replaces the natural D-ring. The new compounds are noncalcemic and show transcriptional and antiproliferative activities similar to 1,25D3. Structural analysis revealed that they induce a large conformational rearrangement of the vitamin D receptor around helix 6.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Oncology
Dorottya Muehl, Magdolna Herold, Zoltan Herold, Lilla Hornyak, Attila Marcell Szasz, Magdolna Dank
Summary: Vitamin D-3 and homocysteine level abnormalities are closely related to the etiology of colorectal cancer. This study explored the longitudinal changes in these parameters in CRC patients and found a swoosh-shaped trend in homocysteine levels. The study also observed that vitamin D-3 levels remained constant or increased in patients without metastasis, but the disease-worsening effect of metastases cancelled out the positive effects of vitamin D-3 in metastatic patients. Right-sided tumors, male sex, and pathological values of serum lipids, albumin, total protein, and inflammatory markers were associated with lower vitamin D-3 and higher homocysteine levels.
Article
Urology & Nephrology
Brittney M. Rush, Corry D. Bondi, Sean D. Stocker, Kacie M. Barry, Sarah A. Small, Jason Ong, Soma Jobbagy, Donna B. Stolz, Sheldon Bastacky, Dionysios Chartoumpekis, Thomas W. Kensler, Roderick J. Tan
Summary: The activation of Nrf2 may exacerbate proteinuria in chronic kidney disease in mice, and is associated with changes in blood pressure and heart rate.
KIDNEY INTERNATIONAL
(2021)
Article
Gastroenterology & Hepatology
Yoko Yagishita, Melissa L. McCallum, Thomas W. Kensler, Nobunao Wakabayashi
Summary: The study revealed that activation of the Nrf2 signaling negatively regulates Math1 in the Notch cascade, leading to excessive enterogenesis in the intestinal epithelium. These two signaling pathways have inverse spatial profiles in the intestinal epithelium, and may play crucial roles in regulating intestinal homeostasis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Multidisciplinary Sciences
Jian-Guo Chen, Jian Zhu, Yong-Hui Zhang, Yong-Sheng Chen, Jian-Hua Lu, Yuan-Rong Zhu, Hai-Zhen Chen, Ai-Guo Shen, Gao-Ren Wang, John D. Groopman, Thomas W. Kensler
Summary: The study tracked the changes in liver cancer mortality rates in Qidong, China, finding that the rates experienced fluctuations from increase to decrease over the past decades. Analysis by age groups and birth cohorts revealed variations in liver cancer mortality rates.
Article
Chemistry, Medicinal
Joshua W. Smith, Robert N. O'Meally, Derek K. Ng, Jian-Guo Chen, Thomas W. Kensler, Robert N. Cole, John D. Groopman
Summary: The study used a novel targeted albumin adductomics platform to monitor Cys(34) adducts in serum samples from 26 nonsmoking women in Qjdong, China over 84 days. Elevated levels of specific adducts were observed with increasing ambient air pollution levels, indicating a perturbation of serum redox balance and potentially increased pollution-related mortality risk. This assay provides a sensitive and multiplexed approach for internal dosimetry of environmental exposures, offering a new strategy for personalized biomonitoring and prevention.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Review
Nutrition & Dietetics
Jed W. Fahey, Thomas W. Kensler
Summary: Broccoli sprouts are a rich source of glucoraphanin, which can generate the chemopreventive agent sulforaphane. Sulforaphane has protective effects against various diseases, but faces challenges in clinical trials, requiring further research and obstacles to overcome.
FRONTIERS IN NUTRITION
(2021)
Article
Biology
Akira Uruno, Daisuke Saigusa, Takafumi Suzuki, Akane Yumoto, Tomohiro Nakamura, Naomi Matsukawa, Takahiro Yamazaki, Ristumi Saito, Keiko Taguchi, Mikiko Suzuki, Norio Suzuki, Akihito Otsuki, Fumiki Katsuoka, Eiji Hishinuma, Risa Okada, Seizo Koshiba, Yoshihisa Tomioka, Ritsuko Shimizu, Masaki Shirakawa, Thomas W. Kensler, Dai Shiba, Masayuki Yamamoto
Summary: The volume of epididymal white adipose tissue and plasma glycerophospholipid and sphingolipid levels in mice increase during spaceflight. These metabolic and physiological changes were largely driven by Nrf2 (NF-E2-related factor-2), ultimately providing a mechanistic target for altering lipid metabolism on Earth and the Great Beyond.
COMMUNICATIONS BIOLOGY
(2021)
Article
Food Science & Technology
Joshua W. Smith, Derek K. Ng, Christian S. Alvarez, Patricia A. Egner, Sean M. Burke, Jian-Guo Chen, Thomas W. Kensler, Jill Koshiol, Alvaro Rivera-Andrade, Maria F. Kroker-Lobos, Manuel Ramirez-Zea, Katherine A. McGlynn, John D. Groopman
Summary: The practice of normalizing aflatoxin B1-lysine adduct concentrations to total circulating human serum albumin (HSA) concentration may not effectively adjust for biological and technical variance, according to empirical analysis. HSA accounts for very little of the between-person variance in HSA-normalized or raw AFB(1)-lysine measurements, and empirical HSA values do not reduce measurement error any better than using simulated data. This suggests that normalizing adducts to HSA concentration may not be necessary when using LCMS in serum/plasma.
Article
Biochemistry & Molecular Biology
Liam Baird, Thomas W. Kensler, Masayuki Yamamoto
Summary: The KEAP1-NRF2 pathway plays a crucial role in human cancer development, leading to aggressive tumors resistant to current treatment regimens. Thus, targeting NRF2-activated cancers with new therapeutic strategies is essential in overcoming treatment resistance.
Article
Biochemistry & Molecular Biology
Yoko Yagishita, Dionysios V. V. Chartoumpekis, Thomas W. W. Kensler, Nobunao Wakabayashi
Summary: The transcription factor NRF2 is a crucial regulator of cellular defense system, activating transcriptional programs of target genes related to redox balance and detoxification. Recent studies have shown its involvement in the regulation of cell fates. However, further research is needed to understand the molecular mechanisms underlying the intersection between NRF2 signaling and cell fate determinations.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemical Research Methods
Joshua W. Smith, Robert N. O'Meally, Sean M. Burke, Derek K. Ng, Jian-Guo Chen, Thomas W. Kensler, John D. Groopman, Robert N. Cole
Summary: Assessing personal exposure to environmental toxicants is crucial for predicting disease risk. A novel Pan-Protein Adductomics (PPA) technology was developed to comprehensively study modifications on human serum albumin (HSA), revealing 68 unique location specific modifications (LSMs) in nonsmoking women exposed to high levels of air pollution.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Pharmacology & Pharmacy
Tonibelle Gatbonton-Schwager, Yoko Yagishita, Tanvi Joshi, Nobunao Wakabayashi, Harini Srinivasan, Takafumi Suzuki, Masayuki Yamamoto, Thomas W. Kensler
Summary: Bardoxolone methyl (CDDO-Me) is a potential treatment for diabetic kidney disease. Studies in mice show that triterpenoids have efficacy against various diseases, including kidney injury, lung injury, and hepatitis. A mutation in the KEAP1 gene disrupts the protective effect of triterpenoids, indicating that the NRF2 pathway is involved in this protection.
MOLECULAR PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nobunao Wakabayashi, Yoko Yagishita, Tanvi Joshi, Thomas W. Kensler
Summary: This study investigated the possibility of activating NRF2 signaling or directly elevating NQO1 levels in the liver to reduce steatosis associated with lipodystrophy. Through hydrodynamic tail vein injection of pCAG-Nrf2 and pCAG-Nqo1 expression vectors in malnourished mice, it was found that both methods protected the liver from damage, with the pCAG-Nqo1 vector being more effective.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yoko Yagishita, Tanvi Joshi, Thomas W. W. Kensler, Nobunao Wakabayashi
Summary: The physiological roles of aryl hydrocarbon receptor (AhR) in the small intestine have been revealed as immunomodulatory and barrier functions. However, its contributions to cell fate regulation are incompletely understood. A study demonstrated that AhR regulates Math1 expression in a xenobiotic response element (XRE)-dependent manner, which highlights a novel molecular basis for fine-tuning Notch-mediated cell fate regulation.
MOLECULAR AND CELLULAR BIOLOGY
(2023)
Article
Oncology
Mark Steven Miller, Peter J. Allen, Powel H. Brown, Andrew T. Chan, Margie L. Clapper, Roderick H. Dashwood, Shadmehr Demehri, Mary L. Disis, Raymond N. DuBois, Robert J. Glynn, Thomas W. Kensler, Seema A. Khan, Bryon D. Johnson, Karen T. Liby, Steven M. Lipkin, Susan R. Mallery, Emmanuelle J. Meuillet, Richard B. S. Roden, Robert E. Schoen, Zelton D. Sharp, Haval Shirwan, Jill M. Siegfried, Chinthalapally V. Rao, Ming You, Eduardo Vilar, Eva Szabo, Altaf Mohammed
Summary: The meeting aimed to facilitate exchanges in cancer prevention research, highlight new trends in immunoprevention and chemoprevention, provide NCI resources to the research community, and cover various stages from basic research to clinical trials.
JOURNAL OF CANCER PREVENTION
(2021)
Article
Oncology
Nichole A. Garzia, Kara Cushing-Haugen, Thomas W. Kensler, Rulla M. Tamimi, Holly R. Harris
Summary: The study found that there were associations between adolescent inflammatory dietary patterns and mammographic density, but these associations disappeared after adjusting for BMI and other risk factors. No independent associations were observed between diet patterns during adolescence and early adulthood with breast density in mid-adulthood.
BREAST CANCER RESEARCH
(2021)