Article
Hematology
Dorothy M. Adcock, Gary W. Moore, Silmara de Lima Montalvao, Geoffrey Kershaw, Robert C. Gosselin
Summary: Mixing studies are useful for investigating prolonged aPTT or PT and differentiating between factor deficiency and inhibitor. However, there is a lack of standardization in interpreting the results and testing protocols.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Article
Genetics & Heredity
Fan Zhang, Guangyan Mu, Zhiyan Liu, Qiufen Xie, Hanxu Zhang, Shuang Zhou, Zhe Wang, Kun Hu, Zining Wang, Xia Zhao, Yimin Cui, Qian Xiang
Summary: This study evaluated the effect of gene polymorphisms on prothrombin time (PT) and activated partial thromboplastin time (APTT) in a healthy Chinese population. It identified ZNF594 and ACTN1 variants as potential genetic markers for PT, and PRTG polymorphisms might be associated with APTT levels.
Article
Veterinary Sciences
Juan A. De Pablo-Moreno, Antonio Liras, Luis Revuelta
Summary: This study aimed to provide an accurate determination and standardization of factor V levels, prothrombin time and activated partial thromboplastin time in Mus musculus. A mouse-based protocol was designed and evaluated in 66 healthy animals using a semi-automated coagulometer and human diagnostic reagents. The most stable period for blood sample extraction was found to be the first 6 h of light. Reference intervals were established for factor V, prothrombin time, and activated partial thromboplastin time. The results have implications for biomedical research and transfusional medicine.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Medical Laboratory Technology
Min Young Lee, Woo In Lee, So Young Kang, Myeong Hee Kim
Summary: This study confirmed that adult-based reference intervals are not applicable for PT in individuals aged 10-18 and aPTT throughout childhood. New pediatric RIs were established, showing higher values than adult-based RIs. Using pediatric RIs can save time, labor, and costs in clinical decision-making.
CLINICAL LABORATORY
(2022)
Article
Hematology
Yao Yuan, Xinyu Li, Feng Qiu
Summary: Activated partial thromboplastin time (aPTT) or prothrombin time (PT) can be prolonged or normal after rivaroxaban administration, but the clinical risk factors and outcomes of prolongation are unknown. In this retrospective study, 155 adult inpatients who had aPTT/PT tested before and after rivaroxaban administration were included. The study found that patient height and receiving human albumin replacement were independent risk factors for mild prolongation of aPTT/PT, but the prolongation did not increase the occurrence of bleeding events.
CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Tze-Fan Chao, Yi-Hsin Chan, Pei-Chien Tsai, Hsin-Fu Lee, Shang-Hung Chang, Chi-Tai Kuo, Gregory Y. H. Lip, Shih-Ann Chen, Yung-Hsin Yeh
Summary: This study investigated the association between prothrombin time and international normalized ratio (PT-INR) or activated partial thromboplastin time (aPTT) ratio and the risks of ischemic stroke/systemic embolism (IS/SE) and major bleeding in patients with atrial fibrillation (AF) taking rivaroxaban or dabigatran. The results showed that a higher INR level was associated with a lower risk of IS/SE in patients treated with rivaroxaban. PT-INR or aPTT ratios were not associated with bleeding events for rivaroxaban or dabigatran, but INR may help predict the outcome of AF patients who take rivaroxaban.
Article
Hematology
Christoph Schriefl, Christian Schoergenhofer, Juergen Grafeneder, Michael Poppe, Christian Clodi, Matthias Mueller, Florian Ettl, Bernd Jilma, Pia Wallmueller, Nina Buchtele, Constantin Weikert, Heidrun Losert, Michael Holzer, Fritz Sterz, Michael Schwameis
Summary: Coagulation abnormalities following successful resuscitation from cardiac arrest may be linked to unfavorable neurologic outcomes. Specifically, prolonged aPTT upon admission is associated with an increased risk of unfavorable neurologic function after successful resuscitation.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Medical Laboratory Technology
Josep Miquel Bauca, Eva Ajzner, Janne Cadamuro, Andreas Hillarp, Ann Helen Kristoffersen, Piet Meijer
Summary: This study assessed the impact of methodological diversity on APTT mixing test results and their interpretation. Despite using different methodologies, most European laboratories obtained valid results. However, the classification of the results could be improved.
CLINICA CHIMICA ACTA
(2022)
Review
Medicine, General & Internal
Evangelia Samara, Konstantinos Stamatiou, Marina Balanika, Petros Tzimas
Summary: Studies suggest that Sugammadex may transiently increase aPTT and PT values, but with minimal clinical impact. Heterogeneity in the trial findings prevents meta-analysis, highlighting the need for more well-designed studies.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Medicine, General & Internal
Wan Hui Wong, Chuen Wen Tan, Nabeelah Binti Abdul Khalid, Nadjwa Zamalek Dalimoenthe, Christina Yip, Chaicharoen Tantanate, Rodelio D. Lim, Ji Hyun Kim, Heng Joo Ng
Summary: Reagent type significantly affects APTT-based clot waveform analysis (CWA), and standardized methods are needed for potential diagnostic use. A study involving 1055 healthy volunteers from the Asia-Pacific region showed that CWA parameters varied less than 10% between reagent lots. Age and gender were found to have a weak correlation with CWA, requiring further investigation.
Article
Chemistry, Physical
Atul N. Khadse, Hardik H. Savsani, Rupesh V. Chikhale, Rahul B. Ghuge, Dixit R. Prajapati, Goshiya Kureshi, Prashant R. Murumkar, Kirti V. Patel, Sadhana J. Rajput, Mange Ram Yadav
Summary: Direct inhibition of FXa has been proven effective in anticoagulation with minimal bleeding risks. A novel series of anthranilamide derivatives were designed and synthesized to improve selectivity and physicochemical properties. These compounds showed potent FXa inhibitory activities and high selectivity over thrombin, as well as good anticoagulant activity and safety in animal models.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Emergency Medicine
Olufemi Emmanuel Idowu, Stevens Olaide Oyeleke, Julius Mautin Vitowanu
Summary: Chronic subdural haematoma (CSH) involves multiple factors in its development and progression. This study found that inflammatory and coagulation indices such as neutrophil to lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), activated partial thromboplastin time (APTT) have predictive value for the severity and outcome of CSH in patients.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Medical Laboratory Technology
Yutaro Yasui, Toshiaki Ishii, Junko Tatebe, Toshisuke Morita
Summary: This study aims to clarify the factors related to the difference in measured values between two APTT reagents. Multiple regression analysis and experimental verification identified albumin-globulin ratio, C-reactive protein, hematocrit, and prothrombin time as factors related to Delta APTT.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Review
Hematology
Rebecca Swayngim, Candice Preslaski, Clay Cothren Burlew, Jacob Beyer
Summary: Review of available evidence comparing aPTT and anti-Xa monitoring for therapeutic UFH did not show a significant difference in clinical outcomes of bleeding, thrombosis, or mortality.
THROMBOSIS RESEARCH
(2021)
Article
Hematology
C. Mary Schooling, Shiu Lun Au Yeung, Jie Zhao
Summary: This study investigated the genetic effects of statins, PCSK9 inhibitors, and alternative lipid targets on key indicators of the coagulation system. Genetically mimicking statins and possibly targeting LDLR may act through the coagulation cascade pathway, potentially leading to new interventions and informing the use of statins in relation to other anticoagulants.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Chemistry, Applied
Dipak P. Mali, Dinanath T. Gaikwad, Manish S. Bhatia, Neela M. Bhatia
Summary: This study aimed to synthesize derivatives from identified plant-based pyridoindole lead scaffold and evaluate their phosphodiesterase 5A inhibitory potential. Through in silico and in vivo studies, three promising molecules were identified as potential antihypertensive agents, with molecule D12 showing the best antihypertensive activity. This research provides a platform for designing novel biomolecules for managing hypertension by inhibiting phosphodiesterase 5A enzyme.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Organic
Nitin Ghatpande, Deepak Phal, Rajshekhar Karpoormath, Mahmoud Soliman, Jagannath Jadhav, Prafulla Choudhari, Mahidansha M. Shaikh
Summary: An efficient method was developed for synthesizing a novel N-acyl/aroyl spiro[chromane-2,4 '-piperidin]-4(3H)-one analogue through two-stage condensation and amidation reactions, resulting in compounds with excellent docking integrations and anti-fungal and anti-microbial activities.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Article
Biochemistry & Molecular Biology
G. Mahaboob Basha, Rishikesh S. Parulekar, Abdullah G. Al-Sehemi, Mehboobali Pannipara, Vidavalur Siddaiah, Sunanda Kumari, Prafulla B. Choudhari, Yasinalli Tamboli
Summary: This study identifies new Maraviroc analogues with potential inhibitory effects on the novel coronavirus and cytokine molecular storm through in vitro research. These analogues, obtained through a systematic drug repurposing strategy, have the potential to be developed into important drugs against COVID-19.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Pharmacology & Pharmacy
Smita Tukaram Kumbhar, Shitalkumar Shivgonda Patil, Manish Sudesh Bhatia
Summary: The study aimed to develop saccharide conjugates for targeted delivery of the cardio-selective beta-blocker metoprolol. The performance, drug release, and cellular uptake properties of modified saccharides and metoprolol-modified saccharide conjugates were evaluated through a series of experiments, and the cytotoxicity of synthesized conjugates was studied using a brine shrimp lethality bioassay.
JOURNAL OF PHARMACEUTICAL INNOVATION
(2022)
Article
Biochemistry & Molecular Biology
Abdullah G. Al-Sehemi, Rishikesh S. Parulekar, Mehboobali Pannipara, Manzur P. P. Ali, Pudukulathan K. Zubaidha, Manish S. Bhatia, Tapan Kumar Mohanta, Ahmed Al-Harrasi
Summary: This study utilized in silico screening method and the 3D structure of SARS-CoV-2 M-pro protein to discover potential inhibitor candidates and investigate their interactions with the substrate binding pocket. The findings suggest that certain furoxan derivatives can serve as both NO donor and SARS-CoV-2 M-pro protein inhibitors, offering a dual mechanism of action for the treatment of SARS-CoV-2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Medicinal
Neela Bhatia, Pooja Shirale, Prafulla Choudhari, Snehal Ashtekar, Sonali Nirankari, Manish Bhatia
Summary: This study successfully achieved selective targeting of breast cancer cells by synthesizing an ester conjugate, thereby reducing the side effects of anticancer drugs.
PHARMACEUTICAL CHEMISTRY JOURNAL
(2022)
Article
Chemistry, Applied
Varun Kishor Bagal, Sanket Somnath Rathod, Muskan Musa Mulla, Swaranjali Chandrakant Pawar, Prafulla Balkrishna Choudhari, Vijaykumar Tanajirao Pawar, Deepak Vamanrao Mahuli
Summary: In this study, molecular docking and molecular dynamics simulation techniques were used to explore the interaction between bioactive molecules from Tinospora cordifolia and Actinidia deliciosa with Human IL-2. Among all docked bioactive molecules, Pygenic acid-B (PubChem CID:146157192) showed the highest negative binding affinity.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Sanket Rathod, Ketaki Shinde, Jaykedar Porlekar, Prafulla Choudhari, Rakesh Dhavale, Deepak Mahuli, Yasinalli Tamboli, Manish Bhatia, Kishan P. Haval, Abdullah G. Al-Sehemi, Mehboobali Pannipara
Summary: Cancer has long been a major health threat, with breast cancer being the most common among women and lung cancer being the most common overall. CDK8 has been identified as a critical oncogenic driver in breast cancer, and screening found that ZINC000005854718 has high binding affinity with CDK8 and drug-like properties. Molecular dynamics simulation confirmed the stable binding of ZINC000005854718 with CDK8.
Article
Pharmacology & Pharmacy
Sunil Harer, Manish Bhatia, Priyanka Sonar
Summary: This article aims to synthesize a new series of 2-phenyl-3-substituted quinazolin-4(3H)-ones fused with an azomethine (-CH=N-) connection to Benzylidene and ethylidene motifs, which show potential antimicrobial and antifungal activities. The tested compounds exhibited strong inhibition of microbial DHFR and antimicrobial action, with some compounds demonstrating potent activity against both bacteria and fungi.
INTERNATIONAL JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2023)
Article
Chemistry, Multidisciplinary
Prakash Bansode, Dattaprasad Pore, Shivaji Tayade, Sandeep Patil, Prafulla Choudhari, Gajanan Rashinkar
Summary: Two series of structurally diverse ferrocene tethered pyrimidobenzothiazoles and pyrimidobenzimidazoles were designed, synthesized, and evaluated for their in vitro anticancer activity against human breast cancer cell lines. Among the synthesized series, five molecules showed significantly higher anticancer activity (GI50 0.018-0.022 μM) against breast carcinoma cell lines MCF-7 and MDA-MB-231 compared to the standard drug doxorubicin (GI50 0.018 μM). Moreover, most of the synthesized compounds exhibited significant antioxidant activity and some demonstrated potent anti-angiogenic effects in in-ovo chick allantoic membrane assay. In-silico molecular docking analysis revealed that the target compounds inhibit heat shock protein90 (Hsp90).
RESULTS IN CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Rakesh P. Dhavale, Rushikesh P. Dhavale, Manish S. Bhatia, Sagar U. Jadhav, Maruti J. Dhanavade, Sagar S. Barale, Sachin Pathak, Vinayak G. Parale, Kailas D. Sonawane
Summary: In this study, magnetic nanoparticles (MNPs) conjugated with the anticancer drug Nintedanib (NIN) via acid-labile imine bond were successfully synthesized and characterized. The drug release experiment showed that NIN was released effectively in an acidic environment. Cytotoxicity tests demonstrated that NIN-conjugated MNPs exhibited high cytotoxicity against cancer cells, indicating good cancer targeting capability. Therefore, NIN-conjugated MNPs could serve as an efficient nanocarrier for cancer targeting.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sanket Rathod, Pooja Chavan, Deepak Mahuli, Sneha Rochlani, Shalini Shinde, Swaranjali Pawar, Prafulla Choudhari, Rakesh Dhavale, Pralhad Mudalkar, Firoj Tamboli
Summary: Cases of drug-resistant tuberculosis (TB) have increased worldwide, posing a major threat to global TB control strategies and human health. The identification of new therapies is urgently needed, and this study used computational methods to screen a library of biogenic chalcones and identified ZINC000005158606 as a potential lead molecule against drug-resistant TB. Further investigations showed that ZINC000005158606 exhibited higher anti-TB activity compared to standard drugs, indicating its potential efficacy against Mycobacterium tuberculosis.
JOURNAL OF MOLECULAR MODELING
(2023)
Article
Chemistry, Applied
Sneha Rochlani, Manish Bhatia, Sanket Rathod, Prafulla Choudhari, Rakesh Dhavale
Summary: Limonoids from citrus have attracted considerable research interest due to their wide range of pharmacological potential. This study focused on the computational exploration of the antiviral potential of three vital limonoids against spike proteins of SARS-CoV-2, Zika virus NS3 helicase, and dengue virus RNA dependent RNA polymerase. The results revealed that Obacunone showed satisfactory results for density functional theory, molecular docking, and MD simulation study.
NATURAL PRODUCT RESEARCH
(2023)
