Review
Pathology
Gisele Correa de Oliveira Zigmundo, Lauren Frenzel Schuch, Tuany Rafaeli Schmidt, Felipe Martins Silveira, Marco Antonio Trevizani Martins, Vinicius Coelho Carrard, Manoela Domingues Martins, Vivian Petersen Wagner
Summary: This study aimed to develop a good practice guide for using 4nitroquinoline-1-oxide (4NQO) as an inducer of oral carcinogenesis in Wistar rats based on a critical review of published studies. The results showed that different concentrations and administration periods of 4NQO can significantly promote tumor development, but there is a lack of description regarding solution care, adverse effects, and the number of losses.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Pharmacology & Pharmacy
Jose Nunes Carneiro Neto, Juliana Maria Sorbo, Carlos Alberto Arcaro Filho, Thais Fernanda Moreira Sabino, Daniel Araki Ribeiro, Iguatemy Lourenco Brunetti, Cleverton Roberto de Andrade
Summary: Our aim was to verify the modulative capacity of TP-4-ol in 4-nitroquinoline-1-oxide induced oral rat cancer. The negative stereoisomer of TP-4-ol showed weaker activity against the human tongue squamous cell line. Animal experiments confirmed that TP-4-ol could inhibit malignant lesions induced by 4-NQO, but it also exhibited kidney toxicity in rats.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Lenore D. Pitstick, Joanna Goral, Ryan A. Schmelter, Christine M. Fuja, Mae J. Ciancio, Matthew Pytynia, Alice Meyer, Jacalyn M. Green
Summary: This study examined the effects of a high-fat diet and sex on liver histopathology in mice treated with 4NQO. The results showed that 4NQO treatment caused histopathological changes in the liver, and a high-fat diet exacerbated these changes. Male mice showed severe steatosis and increased triglyceride content. The findings of this study are important for understanding the impact of tobacco and diet on liver.
Article
Immunology
Satya Ranjan Sahu, Shweta Thakur, Doureradjou Peroumal, Bhabasha Gyanadeep Utkalaja, Abinash Dutta, Premlata Kumari, Ipsita Subhadarsini, Narottam Acharya
Summary: 4-Nitroquinoline N-oxide (4-NQO) is highly mutagenic and genotoxic, and can cause tumor development in epithelial cells. This study explores the interaction between 4-NQO and immune cells, both in vivo and in vitro, and its effect on oral squamous cell carcinoma (OSCC) progression. The results show that 4-NQO induces a decrease in B-cell population in mice and has a more pronounced effect on B cells than T cells. This suggests that in addition to its genotoxicity in oral epithelial cells, 4-NQO also has long-range effects on specific immune cells, leading to cell death and early immunosuppressive response during oral carcinogenesis, which coevolve with tumor development.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pathology
Zaryab Aziz, Mary Kay Washington, Justin Jacobse, Yash Choksi
Summary: A mouse model for esophageal squamous cell carcinoma (ESCC) was created using the carcinogen 4-nitroquinoline 1-oxide (4-NQO) administered orally. An objective scoring system was developed to quantify the severity of 4-NQO-induced murine ESCC by evaluating histopathologic abnormalities and identifying dysplasia, papillomas, and invasion. Quantitative digitized image analysis was used to calculate scores based on the depth and extent of each feature, with weights assigned to reflect cancer severity. The histology scores were significantly different in the three groups, indicating the ability of this method to distinguish dysplasia from carcinoma. This model can be applied to other mice treated with 4-NQO.
VETERINARY PATHOLOGY
(2023)
Article
Medicine, Research & Experimental
Amitava Mukherjee, Michael W. Epperly, Renee Fisher, Donna Shields, Wen Hou, Arjun Pennathur, James Luketich, Hong Wang, Joel S. Greenberger
Summary: This study evaluated the earliest cellular and molecular changes in the esophagus that lead to esophageal cancer using a mouse model. The researchers found a significant increase in the levels of RNA for oncostatin-M in senescent cells of the esophagus from mice treated with the chemical carcinogen 4-NQO and human esophagus samples. The induction of OSM in chemically-induced esophageal cancer in mice correlated with the appearance of senescent cells.
Article
Chemistry, Analytical
Yuanyuan Niu, Jianfeng Zhou, Haohong Lai, Qing Zhou, Shumei Wang, Haiyun Zhai
Summary: In this research, a sensitive and selective electrochemical sensor for detecting 4-NQO was designed. The electrode modified with UiO-66/Zr via electrodeposition showed excellent performance in detecting 4-NQO. The sensor was successfully applied to detect 4-NQO in actual samples with satisfactory recovery results.
MICROCHEMICAL JOURNAL
(2022)
Article
Oncology
Hana Yamaguchi, Miki Hiroi, Kazumasa Mori, Ryosuke Ushio, Ari Matsumoto, Nobuharu Yamamoto, Jun Shimada, Yoshihiro Ohmori
Summary: This study investigated the gene expression kinetics of chemokines/cytokines during oral cancer development in a mouse model using the carcinogen 4NQO. The results showed that both Th1- and Treg-associated chemokines were simultaneously expressed in premalignant lesions, suggesting the initiation of anti-tumor immune responses and immunosuppression in the early stages of OLP development. This study highlights the role of chemokines and cytokines in immune cell recruitment and activation during oral cancer progression.
Article
Oncology
Srimanta Patra, Biswajita Pradhan, Rabindra Nayak, Chhandashree Behera, Laxmidhar Rout, Mrutyunjay Jena, Thomas Efferth, Sujit Kumar Bhutia
Summary: Curcumin and resveratrol, bioactive phytochemicals, have shown promising chemopreventive and chemoprotective effects in cancer therapy. When combined with established anticancer drugs, they enhance anticancer activity while reducing toxicity towards normal organs through improved pharmacokinetics, pharmacodynamics, bioavailability, and metabolism.Development of drug-loaded nanoparticles, nanocarriers, liposomes, and micelles is gaining attention to improve target specificity and drug efficacy in cancer therapeutics.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shuyun Ge, Haiwen Zhou, Zengtong Zhou, Lin Liu, Jianing Lou
Summary: The study aimed to gain insights into the metabolic transformations during oral carcinogenesis by using gas chromatography-mass spectrometry. The results identified 16 metabolites associated with 4NQO-induced rat tongue carcinogenesis, revealing dysregulated arachidonic acid, fatty acid, and glycine metabolism in the animal model. The findings provide a reference for further research on early diagnosis in oral cancer.
Article
Food Science & Technology
Lei Zhang, Zhuo Qu, Aiwei Song, Jianhong Yang, Jianqiang Yu, Wannian Zhang, Chunlin Zhuang
Summary: The study demonstrated that garlic oil is effective in inhibiting tobacco carcinogen-induced lung cancer and protecting cells from damage, suggesting its potential as a novel candidate agent for chemoprevention of lung cancer.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michele Pellegrino, Emilia Bevacqua, Luca Frattaruolo, Anna Rita Cappello, Stefano Aquaro, Paola Tucci
Summary: Prostate cancer is the second most common cancer in men. Higher intake of polyphenols, curcumin, and quercetin has been associated with lower prostate cancer risk. In this study, the combination of curcumin and quercetin showed synergic anticancer and anti-inflammatory effects, inhibiting proliferation, inducing apoptosis, and modulating inflammation-mediated signaling pathways.
Review
Nutrition & Dietetics
Krystyna Fabianowska-Majewska, Agnieszka Kaufman-Szymczyk, Aldona Szymanska-Kolba, Jagoda Jakubik, Grzegorz Majewski, Katarzyna Lubecka
Summary: Curcumin, a bioactive compound derived from turmeric, has shown potential in inhibiting cancer by modulating DNA methylation. It may play a significant role in the treatment of breast cancer.
Review
Biochemistry & Molecular Biology
Lukasz Huminiecki
Summary: This paper presents an updated and contextualized literature review on functional genomic studies of natural phenols in the context of cancer. It suggests the multilevel chemopreventive and anticancer mechanisms of action shared by multiple dietary natural phenols. Specifically, evidence is cited for the multilevel anti-cancer effects of curcumin and resveratrol.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Chenyang Duan, Hanbin Wang, Dian Jiao, Yanqin Geng, Qiaoli Wu, Hua Yan, Chunhui Li
Summary: Curcumin can inhibit oxidative stress and promote neurological recovery after ICH by activating the Nrf2/HO-1 pathway, providing a potential therapeutic target for clinical treatment of ICH.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Engineering, Biomedical
Leonardo Lobo Ribeiro Cavalcante, Antonio Claudio Tedesco, Aline Evangelista Souza-Gabriel, Hiago Salge Borges, Fabiana Almeida Curylofo-Zotti, Silmara Aparecida Milori Corona
Summary: This study aimed to characterize aluminum phthalocyanine chloride (AlClPc) encapsulated in chitosan nanoparticles (CN) and apply it in antimicrobial photodynamic therapy (aPDT) on multispecies biofilm to analyze the antimicrobial activity and lactate production after treatment. The results showed that aPDT mediated by the nanoconjugate AlClPc + CN + L was efficient against Streptococcus mutans, Lactobacillus casei, and Candida albicans biofilms, significantly reducing the viability of the microorganisms and lactate production.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Fernanda Castanheira Goncalves, Bruno Arruda Mascaro, Cassio Rocha Scardueli, Guilherme Jose Pimentel Lopes de Oliveira, Luis Carlos Spolidorio, Rosemary Adriana Chierici Marcantonio
Summary: This study evaluated the effect of strontium ranelate on post-extraction socket healing in rats treated with bisphosphonates. The use of strontium ranelate showed subtle positive effects on connective tissue organization, but had limited impact on bone necrosis and inflammatory infiltration.
Article
Cell Biology
Vinicius de Paiva Goncalves, Joao Paulo Steffens, Carlos Rossa Junior, Luis Carlos Spolidorio
Summary: This study aimed to evaluate the effects of testosterone depletion and supraphysiological testosterone supplementation on early phase wound healing in animals. The results showed that supraphysiological testosterone supplementation improved wound healing by enhancing angiogenesis and reducing wound contraction.
INFLAMMATION RESEARCH
(2022)
Article
Oncology
Marcell Costa de Medeiros, Min Liu, Rajat Banerjee, Emily Bellile, Nisha J. D'Silva, Carlos Rossa Jr
Summary: The study found that galanin secreted by head and neck squamous cell carcinoma (HNSCC) cells exhibits immune-suppressive and pro-tumoral effects. Galanin decreases cell proliferation and expression of pro-inflammatory cytokines, while increasing apoptosis and expression of pro-tumoral cytokines/growth factors. It also suppresses T cell activation and anti-tumoral phenotypes. High expression of galanin is associated with reduced overall survival in HNSCC patients.
Article
Pharmacology & Pharmacy
Cindy Grace Perez-Pacheco, Natalie Aparecida Rodrigues Fernandes, Angelo Constantino Camilli, Danilo Paschoal Ferrarezi, Amanda Favoreto Silva, Mayara Cristina Zunareli, Camila Fernanda Amantino, Fernando Lucas Primo, Morgana Rodrigues Guimaraes-Stabilli, Carlos Rossa Junior
Summary: The aim of this study was to assess the influence of local application of curcumin-loaded nanoparticles on periodontal repair. The results demonstrate that local administration of curcumin-loaded nanoparticles enhanced tissue repair in an experimental model of periodontal repair, promoting bone repair and increasing collagen content.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alvaro Formoso Pelegrin, Vinicius de Paiva Goncalves, Jhonatan de Souza Carvalho, Denise Madalena Palomari Spolidorio, Luis Carlos Spolidorio
Summary: This study aimed to evaluate the inflammatory profile and resolution of inflammation in ligature-induced periodontal inflammation in rats with depletion and/or supraphysiological testosterone replacement. Results showed that supraphysiological testosterone treatment stimulated gingival tissue repair during ligature-induced periodontitis, and this may be related to an anti-inflammatory and pro-resolutive mechanism resulting from the modulatory effect on PGE2 and IL-10.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Laura Andrea Gonzalez Maldonado, Camyla Rodrigues Nascimento, Natalie Aparecida Rodrigues Fernandes, Ana Lidia Pinheiro Silva, Nisha J. D'Silva, Carlos Rossa Jr
Summary: In oral squamous cell carcinoma, macrophages in the tumor microenvironment play a significant role in prognosis and disease survival. This study focuses on the effects of tumor cell-secreted TGF-beta on macrophage phenotype and macrophage-facilitated tumor invasion. The results show that blocking the effects of TGF-beta can affect macrophage phenotype and impact tumor cell invasion and chemotaxis differently.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Oncology
Mauricio Rocha Dourado, Amr Elseragy, Bruno Cesar da Costa, Fabio Haach Teo, Gustavo Narvaes Guimaraes, Renato Assis Machado, Maija Risteli, Wafa Wahbi, Clarissa Araujo Gurgel Rocha, Livia Maris Ribeiro Paranaiba, Wilfredo Alejandro Gonzalez-Arriagada, Sabrina Daniela da Silva, Ana Lucia Carrinho Ayroza Rangel, Marcelo Rocha Marques, Carlos Rossa Junior, Tuula Salo, Ricardo D. Coletta
Summary: In this study, the expression and impact of stress induced phosphoprotein 1 (STIP1) in oral squamous cell carcinoma (OSCC) were investigated. It was found that STIP1 was highly expressed in OSCC, and its overexpression was associated with shorter survival and higher risk of recurrence. Knockdown of STIP1 reduced proliferation, migration, and invasion of tumor cells, and decreased the number of metastases in a zebrafish model. Furthermore, STIP1 was inversely expressed with miR-218-5p, and transfection of miR-218-5p mimics decreased STIP1 levels and inhibited tumor cell proliferation, migration, and invasion.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Camyla Rodrigues Nascimento, Natalie Ap Rodrigues Fernandes, Laura Andrea Gonzalez Maldonado, Carlos Rossa Junior
Summary: Understanding macrophage biology is important for understanding various biological processes and developing new immunomodulatory strategies. This study compared the response of macrophages derived from THP-1 and U937 cells to different polarizing conditions, and found that they have different tendencies towards M1 and M2 phenotypes, with THP-1-derived macrophages being more responsive to M1 stimuli.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Iolanda Augusta Fernandes de Matos, Natalie Aparecida Rodrigues Fernandes, Giovani Cirelli, Mariely Araujo de Godoi, Leticia Ribeiro de Assis, Luis Octavio Regasini, Carlos Rossa Junior, Morgana Rodrigues Guimaraes-Stabili
Summary: In this in vitro study, a newly synthesized chalcone T4 was found to inhibit osteoclast differentiation and activity and stimulate osteogenesis, indicating a promising therapeutic potential in osteolytic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mariana Silva Thomaz, Marcela Nascimento Sertorio, Marcos Leoni Gazarini, Daniel Araki Ribeiro, Luciana Pellegrini Pisani, Marcia Regina Nagaoka
Summary: Non-alcoholic fatty liver is the leading cause of liver disease worldwide. This study investigated the role of kinins in experimental non-alcoholic steatohepatitis (NASH) and found that kinins affect the antioxidant system and reduce superoxide dismutase activity in NASH.
Article
Engineering, Biomedical
Fabiana Almeida Curylofo-Zotti, Viviane De Cassia Oliveira, Analu Rodriguez Marchesin, Hiago Salge Borges, Antonio Claudio Tedesco, Silmara Aparecida Milori Corona
Summary: This study aimed to determine the inhibitory effects of green tea (Gt), EGCG, and nanoformulations containing chitosan (Nchi) and chitosan+green tea (Nchi+Gt) against Streptococcus mutans and Lactobacillus casei. The results showed that Gt at a 1:32 dilution inhibited the growth of S. mutans, while EGCG at a 1:4 dilution completely inhibited the growth of both S. mutans and L. casei. Nanoformulations containing Nchi completely inhibited S. mutans at a 1:4 dilution, whereas different concentrations of Nchi and Nchi+Gt were required to inhibit the growth of L. casei. The selective removal of carious lesion effectively reduced S. mutans counts, and the nanoformulations did not promote any additional antibacterial effect in the remaining dentin.
LASERS IN MEDICAL SCIENCE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Elisandra Marcia Rodrigues, Kennia Scapin Viola, Laura Gonzalez Maldonado, Carlos Rossa-Junior, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho
Summary: Modified formulations of calcium silicate repair materials with additives have been developed to enhance handling, consistency, biocompatibility, and bioactivity. In this study, the effects of these modified materials on human osteoblastic cells were evaluated. The results showed that the modified cements did not have cytotoxic effects and promoted cell viability, gene expression, and mineralization processes.
BRAZILIAN ORAL RESEARCH
(2022)