Article
Biochemistry & Molecular Biology
Momoko Ishida, Rika Ohara, Fuka Miyagawa, Hiroe Kikuzaki, Kosuke Nishi, Hiroyuki Onda, Nanami Yoshino, Takuya Sugahara
Summary: This study identified umbelliferose as the active compound in cumin seeds that suppresses degranulation. Umbelliferose was found to significantly inhibit antigen-induced degranulation. Additionally, raffinose was also found to have similar effects, although less potent.
Article
Immunology
Liyan Niu, Jihao Wei, Xuwen Li, Yongri Jin, Xiaolei Shi
Summary: The study demonstrated that narirutin inhibits the degranulation of RBL-2H3 cells by inhibiting Ca2+ influx and regulating MAPK, NF-kappa B, and Tyrosine kinase signaling pathways. This inhibitory mechanism could lead to a reduction in the release of inflammatory mediators.
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
(2021)
Article
Cell Biology
Tolulope E. Ayo, Pratikshya Adhikari, Hao Xu
Summary: The study reveals that maximal production of TNF in antigen-activated RBL-2H3 cells relies on the regulator of exocytic fusion, Munc13-4. It further demonstrates the involvement of various fusion catalysts in TNF production. The findings suggest that soluble TNF and TNFR1 act as autocrine agents and receptors respectively, promoting TNF proliferation during allergic inflammation.
CELLULAR SIGNALLING
(2023)
Article
Food Science & Technology
Qiao Bai, Xiaopin Feng, Yu Wang, Chunyu Wu, Ye Liu, Jiao Sun, Tianli Yue, Fangyu Long
Summary: This study optimized the ultrasonic-assisted pectinase extraction conditions to obtain the maximum cyclic adenosine monophosphate (cAMP) yield from jujube. The results showed that jujube cAMP regulated the cytokine balance in the allergy pathway and prevented allergy symptoms.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Chemistry, Medicinal
Rika Ohara, Takuya Sugahara, Yuna Sugie, Hiroyuki Onda, Nanami Yoshino, Kosuke Nishi, Momoko Ishida, Hiroe Kikuzaki
Summary: A water-soluble extract of coriander was found to inhibit antigen-induced degranulation of RBL-2H3 cells, and two new compounds were identified as the active components.
Article
Immunology
Satomi Mishima, Marin Sakamoto, Hikaru Kioka, Yuka Nagata, Ryo Suzuki
Summary: This study comprehensively examined the role of VAMP3 in mast cell function and the molecular mechanisms underlying it. The results suggest that VAMP3 mediates granule fusion and promotes granule exocytosis in mast cells. The loss of VAMP3 disrupts plasma membrane homeostasis and enhances Fc epsilon RI-mediated signal transduction. VAMP3 plays a multifunctional role in complex secretory responses in mast cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hironori Higashio, Takuya Yokoyama, Tomoyuki Saino
Summary: In this study, a simple fluorimetry-based degranulation assay using rat basophilic leukemia (RBL-2H3) mast cells was developed. The assay successfully represented the degree and kinetics of degranulation, providing a tool for screening substances regulating mast cell degranulation.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Michiko Yoshii, Ai Kitazaki, Koichiro Ozawa
Summary: This study evaluated the pharmacological effects of simvastatin on the degranulation of mast cells in arteriosclerosis plaques. The results showed that simvastatin inhibits the release of granules from rat basophilic leukemia cells by inhibiting PKC delta translocation and the translocation of small GTPase families Rab and Rho.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2023)
Article
Cell Biology
Ashley Wagner, Syed Benazir Alam, Marianna Kulka
Summary: This study compared the response of different mast cell models to common stimuli and found that factors such as source, age, and biological sex play an important role in phenotypic outcomes and may explain the variability in their function.
CELLULAR IMMUNOLOGY
(2023)
Article
Environmental Sciences
Mingwei Tang, Xiao-San Luo, Weijie Huang, Yuting Pang, Youwei Hong, Jinsheng Chen, Lichun Wu, Kent E. Pinkerton
Summary: PM2.5 pollution is a significant environmental and health concern, especially in China, causing respiratory diseases and altering immune function to induce or worsen allergic diseases. This study investigated temporal and spatial differences in allergic responses to PM2.5 in Nanjing city, China, finding that the composition of PM2.5 and heavy metals and water-soluble ions present directly influenced cell degranulation and inflammation. The findings suggest that the health risks associated with PM2.5, such as from vehicular exhaust and combustion sources, should not be overlooked.
Article
Chemistry, Applied
Jing Ma, Pengyan Tong, Yajing Chen, Yu Wang, Hong Ren, Zhenpeng Gao, Tianli Yue, Fangyu Long
Summary: The study explored the preparation of pectic oligosaccharides (POS) using high hydrostatic pressure-assisted enzymatic (E-HHP) method. It was found that TriGalA and POS could attenuate the release of beta-hexosaminidase and histamine, reduce the production of IL-4, and inhibit extracellular Ca2+ influx of RBL-2H3 cells, indicating POS as a potential inhibitor in regulating mast cell-mediated allergic inflammatory responses.
Article
Food Science & Technology
Wenping Lei, Chengguo Liu, Lina Pan, Can Peng, Jiaqi Wang, Hui Zhou
Summary: This study evaluated the probiotic potential of lactic acid bacteria from different dairy sources and screened probiotics with anti-allergic activity, analyzing their effects on cell signaling pathways and cytokines. Some strains showed potential anti-allergic properties and could be used for alleviating allergic diseases, as demonstrated by their downregulation of histamine and cytokines TNF-alpha and IL-4. Additionally, the probiotics significantly decreased the phosphorylation of Lyn and Syk in RBL-2H3 cells, suggesting their potential for treating allergic and inflammatory diseases.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Seon-Young Min, Che-Hwon Park, Hye-Won Yu, Young-Jin Park
Summary: Saponarin, a flavone found in young green barley leaves, demonstrates anti-inflammatory, anti-allergic, and skin-protective effects, making it a potential functional ingredient in cosmetics. Its inhibitory effects on cytokine expression and signaling pathways in different types of cells suggest utility in preventing and treating immune-related skin diseases like atopic dermatitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Miki Hiemori-Kondo, Etsuko Morikawa, Michiko Fujikura, Ayuko Nagayasu, Yuuki Maekawa
Summary: Black soybean hull extract (BSHE) was found to inhibit IgE-mediated type I allergic reactions, with cyanidin-3-O-glucoside (C3G) identified as one of its active compounds. Both oral and intravenous administration of C3G showed suppression of the passive cutaneous anaphylaxis (PCA) reaction.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Chemistry, Applied
Yan Liu, Zhendan Shu, Yan Li, Huiying Chen, Hong Liu, Xianwen Yang, Guangming Liu, Qingmei Liu
Summary: Our previous studies showed that viridicatol from the deep-sea-derived fungus Penicillium griseofulvum can regulate mast cell stabilization and relieve food allergy. To understand the molecular mechanisms, transcriptomes of viridicatol-treated RBL-2H3 cells were analyzed. Differentially expressed genes and enrichment analysis indicated that viridicatol played a significant role in mast cell stabilization by affecting MAPK and JAK-STAT signaling pathways.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2023)