Article
Chemistry, Multidisciplinary
Patrick M. Killoran, George S. M. Hanson, Sanne J. M. Verhoork, Madeleine Smith, Davide Del Gobbo, Lu-Yun Lian, Christopher R. Coxon
Summary: A method using 4-fluorophenylalanine as a reporter group for F-19 NMR was developed to measure the conformational status of peptidylprolyl bonds in peptide models. This method was applied to study the prolyl bond status in pentapeptide models of the intrinsically disordered region of alpha-synuclein. It was demonstrated that F-19 NMR is a valuable tool for studying proline isomerism under different conditions.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Review
Immunology
Sreekanth Rajan, Ho Sup Yoon
Summary: Malaria, a prevalent infectious disease, poses a serious challenge due to drug-resistant strains. Adopting a chemo-genomics approach can uncover novel targets and mechanisms of action for better drug targeting. Immunosuppressive drugs have been found to inhibit the growth of the malarial parasite, with peptidyl prolyl cis/trans isomerases (PPIases) as the specific targets. This review summarizes the structural insights on Plasmodium PPIases, their inhibitor complexes, and perspectives on drug discovery.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biophysics
Silvia Napolitano, Aditya Pokharna, Rudi Glockshuber, Alvar D. Gossert
Summary: The study reveals that certain proline residues in Trx can accelerate the rate-limiting trans-to-cis proline isomerization during protein folding, with trans-P68 playing a critical role in enhancing Trx folding kinetics.
BIOPHYSICAL JOURNAL
(2021)
Article
Multidisciplinary Sciences
Mangaljeet Singh, Kirandeep Kaur, Avinash Sharma, Rajvir Kaur, Dimple Joshi, Megha Chatterjee, Iman Dandapath, Amarjeet Kaur, Harpreet Singh, Prabhjeet Singh
Summary: Peptidyl-prolyl cis-trans isomerases (PPIases) are enzymes capable of cis-trans isomerization of the prolyl peptide bond, playing essential roles in various cellular processes. This study conducted a genome-wide analysis of PPIases in different Penicillium spp. and found variations in gene numbers and activities under salt stress. The halotolerant strain P. oxalicum showed significant enhancement in mycelial PPIase activity under salt stress, suggesting the importance of these enzymes in salt stress adaptation.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Marko Rodewald, J. Mikko Rautiainen, Helmar Goerls, Raija Oilunkaniemi, Wolfgang Weigand, Risto S. Laitinen
Summary: A new platinum complex was synthesized and its crystal structure was analyzed. It was found that the ligand undergoes isomerization and forms a dynamic equilibrium during the dissolution process. Two other platinum complexes were also successfully synthesized and their structures were characterized. The bonding and formation of these complexes were further discussed through theoretical calculations.
Article
Agriculture, Multidisciplinary
Baixi Zhang, Lijuan Ni, Xiaoshu Tang, Xuemei Chen, Bo Hu
Summary: This study achieved a high yield of t10, c12-CLA through genetic modification and reduced its degradation rate. The highest t10, c12-CLA yield reported to date was obtained in a bioreactor using low-cost carbon sources.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Physical
Alexey S. Kiryutin, Vitaly P. Kozinenko, Alexandra V. Yurkovskaya
Summary: This study demonstrates a significant enhancement of nuclear spin polarization of cis and trans isomers of azobenzene by utilizing reversible exchange and simultaneous interaction with parahydrogen molecules and light irradiation. The approach of coherent polarization transfer allows for direct hyperpolarization of cis-azobenzene from parahydrogen in SABRE. Additionally, the proposed method, referred to as photo-SABRE, enables hyperpolarization of the long-lived spin order of trans-isomer N-15 spins.
Article
Genetics & Heredity
Huadi Shi, Fulan Zhong, Xiaoqiong Yi, Zhenyi Shi, Feiyan Ou, Yufang Zuo, Zumin Xu
Summary: A prognostic model based on 3 peptidyl prolyl cis-trans isomerase gene signatures was constructed for hepatocellular carcinoma patients. The model showed that patients in the high-risk score group had a worse prognosis, and the validation results were consistent with the training set. A nomogram incorporating clinical stage and risk score was established with a well-matched calibration curve.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Multidisciplinary
Jugal Kishore Rai Deka, Debashree Borah, Paramesh Das, Biswajit Sahariah, Pratap Vishnoi, Bani Kanta Sarma
Summary: Carbonyl-carbonyl (COMIDLINE HORIZONTAL ELLIPSISCO) n -> pi* interaction often coexists with a hydrogen bond or another n -> pi* interaction. In this study, a set of proline-diacylhydrazine molecules were investigated, and it was found that the increase in the strength of the n(N) -> pi*(Ar) interaction on the DAH side strengthened the n -> pi* interaction on the Pro side. This research provides a simple C-terminal modification strategy to stabilize the trans-Pro geometry, which is important for stabilizing PPII helices and collagen triple helices.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Mauro Ravera, Elisabetta Gabano, Ilaria Zanellato, Beatrice Rangone, Elena Perin, Beatrice Ferrari, Maria Grazia Bottone, Domenico Osella
Summary: Two Pt(iv) conjugates containing perillic acid were successfully synthesized, with one showing excellent antiproliferative and antimetastatic activity on lung tumor cells, and various in vitro biological tests were conducted to decipher some aspects of its mechanism of action.
DALTON TRANSACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Mickael Mauger, Carla Ferreri, Chryssostomos Chatgilialoglu, Myriam Seemann
Summary: Bacteria have evolved various strategies to resist threats, one of which involves utilizing fatty acids to build membranes to combat environmental pressures. Specific enzymes can catalyze the isomerization of fatty acids to reduce membrane fluidity in response to danger.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Nabil Al-Zaqri, Ali Alsalme, Fahad Alharthi, Afnan Al-Taleb, Ahmed M. Boshaala, Ahmed Chetouni, Abdelkader Zarrouk, Ismail Warad
Summary: A novel BDMDO monomer was prepared and characterized in this study, revealing its molecular structure and properties through various analyses and computations. The findings contribute to a better understanding of the chemical characteristics and potential applications of BDMDO.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Chemistry, Physical
Abhigyan Sengupta, Lorenz E. Rognoni, Ulrich Merkel, Gabriel Zoldak, Matthias Rief
Summary: Prolyl isomerization catalyzed by the PPIase enzyme plays a crucial role in cellular signaling and molecular timing. This study demonstrates a 25-fold acceleration of trans-cis isomerization rate and a stabilization effect on the cis-isomer by SlyD in a single-molecule experiment with FlnA20 protein.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Fanni Sebak, Janos Szolomajer, Nandor Papp, Gabor K. Toth, Andrea Bodor
Summary: The type of neighboring amino acids, especially aromatic and positively charged sidechains, has been found to affect the cis-Pro content. These findings can assist in the design of protein regions with specific cis-Pro content and contribute to a better understanding of the roles and functions of IDPs.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
Article
Immunology
Nikhil Ponnoor Anto, Amitha Muraleedharan, Pulak Ranjan Nath, Zuoming Sun, Chen Keasar, Etta Livneh, Alex Braiman, Amnon Altman, Kok-Fai Kong, Noah Isakov
Summary: Protein kinase C-α (PKC-α) is selectively expressed in T lymphocytes and plays a crucial role in T cell activation and proliferation. The phosphorylation of Thr(335)-Pro residue in the regulatory domain of PKC-α is important for its activation and localization at the immunological synapse. The peptidyl-prolyl cis-trans isomerase Pin1 interacts with PKC-α through the phosphorylated Thr(335)-Pro motif, and this interaction is involved in early activation events in TCR-triggered T cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Amaravadhi Harikishore, Ho Sup Yoon
CURRENT MOLECULAR PHARMACOLOGY
(2016)
Article
Chemistry, Multidisciplinary
Chengbin Yang, Nishtha Panwar, Yucheng Wang, Butian Zhang, Maixian Liu, Huiting Toh, Ho Sup Yoon, Swee Chuan Tjin, Peter Han Joo Chong, Wing-Cheung Law, Chih-Kuang Chen, Ken-Tye Yong
Article
Biochemistry & Molecular Biology
Ajit Prakash, Joon Shin, Sreekanth Rajan, Ho Sup Yoon
NUCLEIC ACIDS RESEARCH
(2016)
Article
Biochemistry & Molecular Biology
Ajit Prakash, Sreekanth Rajan, Ho Sup Yoon
Article
Chemistry, Physical
Chengbin Yang, Kok Ken Chan, Wen-Jen Lin, Alana Mauluidy Soehartono, Guimiao Lin, Huiting Toh, Ho Sup Yoon, Chih-Kuang Chen, Ken-Tye Yong
Article
Optics
Hamid Farrokhi, Jeeranan Boonruangkan, Byung Jae Chun, Thazhe Madam Rohith, Abhinay Mishra, Hui Ting Toh, Ho Sup Yoon, Young-Jin Kim
Article
Chemistry, Multidisciplinary
Xumeng Wu, Yu Jia Tan, Hui Ting Toh, Lan Huong Nguyen, Shu Hui Kho, Sing Yian Chew, Ho Sup Yoon, Xue-Wei Liu
Article
Plant Sciences
Geeta Kumari, Ka Ho Wong, Aida Serra, Joon Shin, Ho Sup Yoon, Siu Kwan Sze, James P. Tam
Article
Biotechnology & Applied Microbiology
Serap Beldar, Malathy Sony Subramanian Manimekalai, Nam-Joon Cho, Kwanghee Baek, Gerhard Gruber, Ho Sup Yoon
JOURNAL OF GENERAL VIROLOGY
(2018)
Article
Chemistry, Medicinal
Sebastian Pomplun, Claudia Sippel, Andreas Haehle, Donald Tay, Kensuke Shima, Alina Klages, Can Murat Uenal, Benedikt Riess, Hui Ting Toh, Guido Hansen, Ho Sup Yoon, Andreas Bracher, Peter Preiser, Jan Rupp, Michael Steinert, Felix Hausch
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Yeen Shian Ngow, Sreekanth Rajan, Hong Ye, Ho Sup Yoon
Article
Chemistry, Physical
Qingling Ouyang, Xueling Feng, Shuangyang Kuang, Nishtha Panwar, Peiyi Song, Chengbin Yang, Guang Yang, Xinya Hemu, Gong Zhang, Ho Sup Yoon, James P. Tam, Bo Liedberg, Guang Zhu, Ken-Tye Yong, Zhong Lin Wang
Article
Multidisciplinary Sciences
Tae-Yoon Park, Yongwoo Jang, Woori Kim, Joon Shin, Hui Ting Toh, Chun-Hyung Kim, Ho Sup Yoon, Pierre Leblanc, Kwang-Soo Kim
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Sreekanth Rajan, Yongwoo Jang, Chun-Hyung Kim, Woori Kim, Hui Ting Toh, Jeha Jeon, Bin Song, Aida Serra, Julien Lescar, Jun Yeob Yoo, Serap Beldar, Hong Ye, Congbao Kang, Xue-Wei Liu, Melissa Feitosa, Yeahan Kim, Dabin Hwang, Geraldine Goh, Kah-Leong Lim, Hye Min Park, Choong Hwan Lee, Sungwhan F. Oh, Gregory A. Petsko, Ho Sup Yoon, Kwang-Soo Kim
NATURE CHEMICAL BIOLOGY
(2020)
Article
Pharmacology & Pharmacy
Hanjie Jonathan Gan, Amaravadhi Harikishore, Jihye Lee, Sangeun Jeon, Sreekanth Rajan, Ming Wei Chen, Jun Long Neo, Seungtaek Kim, Ho Sup Yoon
Summary: MERS, caused by a coronavirus, has a high mortality rate and lacks specific treatments. Research has found that montelukast sodium hydrate (MSH), an FDA-approved anti-asthma drug, can inhibit MERS-CoV infection, potentially serving as a candidate for therapeutic development against MERS-CoV infections.
ANTIVIRAL RESEARCH
(2021)