期刊
BIOMICROFLUIDICS
卷 7, 期 1, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4788914
关键词
biomedical engineering; bioMEMS; blood; cancer; cellular transport; fractionation; haemodynamics; microchannel flow; orifices (mechanical); tumours
资金
- Korea Science & Engineering Foundation (KOSEF)
- Korean government (MEST) [2008-05943, 2011-0016731]
- National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea [1120290]
- Korea Health Promotion Institute [1120290] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2011-0016731] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer; as such, the ability to isolate and quantify CTCs is of great biomedical importance. This research presents a multi-stage multi-orifice flow fractionation (MS-MOFF) device formed by combining three single-stage multi-orifice segments designed for separating breast cancer cells from blood. The structure and dimensions of the MS-MOFF were determined by hydrodynamic principles to have consistent Reynolds numbers (Re) at each multi-orifice segment. From this device, we achieved improved separation efficiency by collecting and re-separating non-selected target cells in comparison with the single-stage multi-orifice flow fractionation (SS-MOFF). The recovery of breast cancer cells increased from 88.8% to greater than 98.9% through the multi-stage multi-orifice segments. This device can be utilized to isolate rare cells from human blood, such as CTCs, in a label-free manner solely through the use of hydrodynamic forces. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4788914]
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