期刊
BIOMICROFLUIDICS
卷 3, 期 1, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3055278
关键词
bioMEMS; cellular biophysics; computational fluid dynamics; hydrodynamics; microchannel flow; optical microscopy; stroboscopes
资金
- National Institutes of Health [HG-01389]
Vertical hydrodynamic focusing in microfluidic devices is investigated through simulation and through direct experimental verification using a confocal microscope and a novel form of stroboscopic imaging. Optimization for microfluidic cytometry of biological cells is examined. By combining multiple crossing junctions, it is possible to confine cells to a single analytic layer of interest. Subtractive flows are investigated as a means to move the analysis layer vertically in the channel and to correct the flatness of this layer. The simulation software (ADINA and Coventor) is shown to accurately capture the complex dependencies of the layer interfaces, which vary strongly with channel geometry and relative flow rates.
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