期刊
BIOMETALS
卷 24, 期 1, 页码 93-103出版社
SPRINGER
DOI: 10.1007/s10534-010-9377-2
关键词
Alfalfa seedling roots; Ascorbic acid; Cd-induced oxidative stress; Glutathione depletion; HO-1 gene expression
资金
- Program for New Century Excellent Talents in University [NCET-07-0441]
- National Natural Science Foundation of China [30971711]
- Fundamental Research Funds for the Central Universities [KYZ200905]
Following previous findings that cadmium (Cd) induces heme oxygenase-1 (HO1) gene expression in alfalfa seedling roots, we now show that the decreased glutathione (GSH) and ascorbic acid (AsA) contents, induction of HO-1 gene expression and its protein level by Cd was mimicked by a GSH depletor diethylmaleate (DEM). Meanwhile, above Cd- or DEM-induced decreased GSH content followed by HO-1 up-regulation could be strengthened or reversed differentially by the application of a selective inhibitor of GSH biosynthesis l-buthionine-sulfoximine (BSO), or exogenous GSH and AsA, respectively. The antioxidative behavior of HO-1 induction was further confirmed by histochemical staining for the detection of loss of membrane integrity in a short period of treatment time. Additionally, the induction of HO-1 transcript was inhibited by the transcriptional inhibitor actinomycin D (ActD) or protein synthesis inhibitor cycloheximide (CX, especially). In contrast, the level of HO-2 transcript did not change upon various treatments. Together, above results suggested that Cd-induced up-regulation of HO-1 gene expression is associated with GSH depletion, which is at least existing transcriptional regulation level, thus leading to enhanced antioxidative capability transiently.
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