Article
Biochemistry & Molecular Biology
Anna Yu. Elizarova, Alexey V. Sokolov, Vadim B. Vasilyev
Summary: The LF/8OA complex has been found to inhibit tumor growth, but its effects on normal blood cells and modulation of CP have not been studied. The experimental results show that the LF/8OA complex can cause hemolysis and activation of neutrophils, while CP can counteract its cytotoxic effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Wei Yang, Xiaoqing Qu, Chujun Deng, Lei Dai, Haoyu Zhou, Guihua Xu, Bo Li, Nazarenko Yulia, Changzhong Liu
Summary: This study investigated the synergistic enhancement in the thermal co-aggregation and gelation of lactoferrin and alpha-lactalbumin under specific conditions. It found that there is a strong rheological synergism between the two proteins, leading to reduced gelation times. The research enhances understanding of heat sensitive protein-heat stable protein thermal aggregation and gelation processes.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Paziewska-Nowak, Marcin Urbanowicz, Kamila Sadowska, Dorota Genowefa Pijanowska
Summary: The paper describes the development and utility of a novel DNA oligonucleotide-based affinity bioreceptor for lactoferrin. The research investigates the affinity of different types of oligonucleotides to the target protein using surface plasmon resonance method. The selected DNA sequence showed high affinity to lactoferrin and demonstrated its potential as a bioreceptor for lactoferrin determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yan Liu, Fan Zhang, Ling Jiang, J. Jefferson P. Perry, Zhihe Zhao, Jiayu Liao
Summary: This study introduces a novel method to determine kinetics parameters of product inhibition using quantitative FRET(qFRET) assay, providing a convenient way to determine all kinetics parameters and a new approach to combine different measurements with mutually compatible results and errors for the first time.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Fisheries
Wang-Jing Liu, Yun-Shiang Chang, Kou-Chun Tseng, Meng-Hua Yu
Summary: White spot syndrome virus (WSSV) is a prominent pathogen in shrimp farming worldwide. However, there is currently no effective treatment for this virus. In this study, the antiviral properties of bovine lactoferrin (bLF) against WSSV were investigated, and it was found that bLF treatment significantly reduced shrimp mortalities caused by WSSV infection. The ability of bLF to bind to both host cells and WSSV virions suggests its potential as an anti-WSSV agent in shrimp culture, and the identified interaction proteins may play a role in WSSV infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Da Chen, Osvaldo H. Campanella
Summary: In this study, pea protein hydrolysates were obtained through enzymatic hydrolysis, and their physicochemical properties and gelling capacity were investigated. The hydrolysates showed decreased fluorescence intensity and surface hydrophobicity compared to the original protein solution, indicating increased water accessibility and polarity. The hydrolysates were unstable at high temperatures but formed gels under specific conditions. Addition of certain substances decreased the gel strength, suggesting the involvement of electrostatic and hydrophobic interactions, as well as hydrogen and disulfide bonds in gelation.
FOOD HYDROCOLLOIDS
(2022)
Review
Agricultural Engineering
Hongliang Guo, Ying Zhao, Jo-Shu Chang, Duu-Jong Lee
Summary: Enzymatic hydrolysis is crucial for efficient biorefinery of lignocellulosic biomass. Understanding the properties of key enzymes and their interactions with biomass is essential for improving the process efficiency. This article provides an update on the research of key enzymes in lignocellulose biorefinery, including their properties, interactions with biomass, adsorption, and hydrolysis. The advanced analytical techniques for investigating these interactions and future research prospects are discussed.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tetsuya Sakajiri, Masatoshi Nakatsuji, Yoshiaki Teraoka, Kosuke Furuta, Katsuya Ikuta, Kotoe Shibusa, Eriko Sugano, Hiroshi Tomita, Takashi Inui, Takaki Yamamura
Summary: The study investigates the interaction mechanism between ceruloplasmin and transferrin mediated by zinc. The findings suggest that zinc may play a role in the transfer of iron between ceruloplasmin and transferrin.
Article
Chemistry, Applied
Bei Korpela, Leena Pitkanen, Marina Heinonen
Summary: The effect of enzyme treatment on protein-tannin interactions was investigated using modern analytical methods. It was found that the formation of ligands between procyanidin B2 and native oat globulin was influenced by the ratio of procyanidin B2 to OG and the availability of tryptophan. The study also revealed that procyanidin B2 inhibited protein-protein interactions and aggregation.
Review
Chemistry, Multidisciplinary
Yilin Hu, Chi Chung Lee, Mario Grosch, Joseph B. Solomon, Wolfgang Weigand, Markus W. Ribbe
Summary: The Fischer-Tropsch (FT) process is used to convert a mixture of CO and H2 into liquid hydrocarbons for synthetic fuel production. However, enzymatic FT-type reactions, catalyzed by nitrogenase enzymes, metalloclusters and synthetic mimics, can also convert CO, CO2, and CN- into hydrocarbons under ambient conditions using H+ and e- as reducing equivalents. This review provides an overview of nitrogenase enzymes and metalloclusters, discusses the activities of nitrogenase-derived FT systems, and explores the mechanistic, evolutionary, and biotechnological implications of enzymatic FT reactions.
Article
Chemistry, Applied
Hanbin Xu, Jiaping Zhou, Jinglin Yu, Shuo Wang, Shujun Wang
Summary: The study found that gluten and its hydrolysates decreased wheat starch digestibility by inhibiting the activity of α-amylase and interacting with starch through hydrogen bonding. Additionally, hydrolyzed gluten GH-1 displayed a greater inhibitory effect and stronger interaction with starch than other gluten/hydrolysates, indicating a significant impact on starch digestibility.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Applied
Taotao Dai, David Julian McClements, Ting Hu, Jun Chen, Xuemei He, Chengmei Liu, Jinfeng Sheng, Jian Sun
Summary: This study investigates the interactions between bovine lactoferrin and tannic acid and their effects on foam properties. Hydrophobic forces are found to be the primary forces holding the complexes together. Additionally, the complex exhibits good foam stability but poor foamability, which is closely related to its interactions with polyphenols.
Article
Biochemistry & Molecular Biology
Zhitao Mao, Xin Zhao, Xue Yang, Peiji Zhang, Jiawei Du, Qianqian Yuan, Hongwu Ma
Summary: Genome-scale metabolic models (GEMs) are widely used for predicting the phenotypes of microorganisms. However, the lack of other constraints in the stoichiometric model often limits the accessibility of the metabolic solution space. In this study, we developed an enzyme-constrained metabolic network model (ECMpy) and applied it to Escherichia coli (E. coli) to improve the accuracy of phenotype predictions. By considering the total enzyme amount constraint, protein subunit composition, and enzyme kinetic parameters, we were able to better predict the overflow metabolism and growth rates of E. coli. The enzyme-constrained model revealed the tradeoff between enzyme usage efficiency and biomass yield, providing valuable insights for metabolic engineering.
Article
Chemistry, Multidisciplinary
Przemyslaw Gajda-Morszewski, Anna Poznanska, Cristina Yus, Manuel Arruebo, Malgorzata Brindell
Summary: Lactoferrin is a glycoprotein found in milk with various therapeutic properties. This study aimed to optimize the encapsulation of lactoferrin to protect it during delivery to the intestines. The encapsulated lactoferrin demonstrated high efficiency and suitable morphology, and partially preserved its integrity and iron scavenging properties against enzymatic digestion.
Article
Chemistry, Applied
Negar Gharbi, Debbie Stone, Nahuel Fittipaldi, Sharon Unger, Deborah L. O'Connor, Yves Pouliot, Alain Doyen
Summary: This study evaluated the effect of different pasteurization methods on protein aggregates in human milk. The results showed that compared to traditional pasteurization, high hydrostatic pressure had a milder effect on protein aggregation, preserving the native structure and bioactivity of the milk proteins.
Article
Biochemistry & Molecular Biology
V. B. Vasilyev, A. Sokolov, V. A. Kostevich, A. Yu Elizarova, N. P. Gorbunov, O. M. Panasenko
Summary: Myeloperoxidase (MPO) interacts with low-density lipoproteins (LDL), leading to atherosclerosis, while lactoferrin (LF) has the potential to inhibit this process, making it a potential therapy for atherosclerosis.
BIOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Zhe He, Weiwei Qiu, Megan E. Kizer, Jizhou Wang, Wencong Chen, Alexei Sokolov, Xing Wang, Jonathan Hu, Marlan O. Scully
Summary: This paper demonstrates a direct method to read RNA sequences by tip-enhanced Raman scattering (TERS) technique and RNA imaging at the single-base level, without the need for RNA labeling or reverse transcriptase RT-PCR amplification.
Review
Biochemistry & Molecular Biology
Jennet T. Mammedova, Alexey V. Sokolov, Irina S. Freidlin, Eleonora A. Starikova
Summary: L-arginine is a key metabolite for nitric oxide production and plays a role in the mTOR signaling pathway. Disruption of L-arginine metabolism in endothelial cells leads to endothelial dysfunction, and the use of L-arginine supplements to improve endothelial function has conflicting results.
BIOCHEMISTRY-MOSCOW
(2021)
Article
Biology
Daria S. Morozova, Alexey A. Martyanov, Sergei Obydennyi, Julia-Jessica D. Korobkin, Alexey Sokolov, Ekaterina Shamova, Irina Gorudko, Anna L. Khoreva, Anna Shcherbina, Mikhail A. Panteleev, Anastasia N. Sveshnikova
Summary: In this study, an ex vivo experimental model for observation of granulocyte activity in thrombus formation was developed. Results showed that granulocytes from patients with WAS had reduced motility compared to healthy donors. The study suggests that WASP deficiency disrupts granulocyte behavior in thromboinflammatory conditions.
Article
Biochemistry & Molecular Biology
Daria Grigorieva, Irina Gorudko, Natalia A. Grudinina, Oleg M. Panasenko, Igor Semak, Alexey Sokolov, Alexander Timoshenko
Summary: LTF can be modified by hypohalous acids, affecting the functional responses of neutrophils. After halogenative modification, LTF lost some of its abilities, but still showed inhibitory effects on certain neutrophil responses like respiratory burst. Halogenative modification of LTF plays a role in regulating neutrophil signaling pathways and pro-inflammatory activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Virology
Alexey Sokolov, Irina Isakova-Sivak, Natalia Grudinina, Daria Mezhenskaya, Elena Litasova, Valeria Kostevich, Ekaterina Stepanova, Alexandra Rak, Ivan Sychev, Olga Kirik, Larisa Rudenko
Summary: The degradation of transferrin receptor 1 (TfR1) by ferristatin II inhibits the entry and replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This finding suggests a promising therapeutic strategy for COVID-19.
Article
Nutrition & Dietetics
Alexey Sokolov, Nadezhda M. Dubrovskaya, Valeria A. Kostevich, Dmitrii S. Vasilev, Irina V. Voynova, Elena T. Zakharova, Olga L. Runova, Igor Semak, Alexander Budevich, Natalia N. Nalivaeva, Vadim B. Vasilyev
Summary: The protective effects of apo-rhLF on the cognitive functions of rat offspring subjected to prenatal hypoxia have been analyzed. The results showed that apo-rhLF can rescue cognitive deficits caused by prenatal hypoxia and improve memory.
Review
Biochemistry & Molecular Biology
V. E. Reut, I. V. Gorudko, D. V. Grigorieva, A. V. Sokolov, O. M. Panasenko
Summary: Hypochlorous acid (HOCl) plays a crucial role in the immune system but its high reactivity can also lead to the development of diseases. Therefore, developing highly sensitive and selective HOCl biosensors is important. This review summarizes the achievements in the development of chemosensors for HOCl detection in biological systems from 2016 to 2021. The challenges in designing and applying small-molecule probes are discussed, and recommendations are given for their future application.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Veronika E. Reut, Stanislav O. Kozlov, Igor Kudryavtsev, Natalya A. Grudinina, Valeria A. Kostevich, Nikolay P. Gorbunov, Daria Grigorieva, Julia A. Kalvinkovskaya, Sergey B. Bushuk, Elena Yu Varfolomeeva, Natalia D. Fedorova, Irina Gorudko, Oleg M. Panasenko, Vadim B. Vasilyev, Alexey Sokolov
Summary: This paper introduces a fluorescent probe called Celestine Blue B (CB), which can be used for HOCl detection in living cells and for assaying the chlorinating activity of MPO. The probe shows remarkable selectivity and sensitivity, and has a rapid response to HOCl. It can also detect endogenous HOCl and reactive halogenated species using various techniques.
Article
Biochemistry & Molecular Biology
A. Sokolov, I. N. Isakova-Sivak, D. A. Mezhenskaya, V. A. Kostevich, N. P. Gorbunov, A. Yu Elizarova, V. A. Matyushenko, Yu M. Berson, N. A. Grudinina, N. N. Kolmakov, Y. A. Zabrodskaya, A. S. Komlev, I. Semak, A. Budevich, L. G. Rudenko, V. B. Vasilyev
Summary: The pathogenesis of SARS-CoV-2 infection involves dysregulations of iron metabolism. Spike protein competes with transferrin receptor 1 (TfR1) ligands and shows cross-reactivity with lactoferrin (Lf) and transferrin (Tf). Apo-Lf and holo-Lf can inhibit SARS-CoV-2 replication and protect against COVID-19-induced pathogenesis.
Article
Biochemistry & Molecular Biology
Oleg M. Panasenko, Viktor A. Ivanov, Elena V. Mikhalchik, Irina V. Gorudko, Daria V. Grigorieva, Liliya Yu. Basyreva, Ekaterina V. Shmeleva, Sergey A. Gusev, Valeria A. Kostevich, Nikolay P. Gorbunov, Alexey V. Sokolov
Summary: Hyperglycemia in diabetes mellitus alters the functionality of neutrophils, reducing their bactericidal activity mediated by MPO, which is affected by the modification of proteins by glucose and its derivative methylglyoxal. This phenomenon may contribute to impaired wound healing and increased susceptibility to infection in patients with hyperglycemia.
Article
Biochemistry & Molecular Biology
Tatyana V. Vakhrusheva, Alexey V. Sokolov, Grigoriy D. Moroz, Valeria A. Kostevich, Nikolay P. Gorbunov, Igor P. Smirnov, Ekaterina N. Grafskaia, Ivan A. Latsis, Oleg M. Panasenko, Vassili N. Lazarev
Summary: Cationic antimicrobial peptides (CAMPs) have potential as antimicrobial therapeutics with low bacterial resistance. However, their interaction with biologically important proteins lacks research. This study found that CAMPs may have a dual role as antimicrobial agents and regulators of MPO activation.
Article
Biochemistry & Molecular Biology
Elena V. Mikhalchik, Liliya N. Maltseva, Roxalana K. Firova, Marina A. Murina, Irina V. Gorudko, Daria V. Grigorieva, Viktor A. Ivanov, Ekaterina A. Obraztsova, Dmitry V. Klinov, Ekaterina V. Shmeleva, Sergey A. Gusev, Oleg M. Panasenko, Alexey V. Sokolov, Nikolay P. Gorbunov, Lyubov Y. Filatova, Nadezhda G. Balabushevich
Summary: This study investigated the effect of incorporating pectin into vaterite microparticles on cytotoxicity and mucin-dependent neutrophil activation. The results showed that the inclusion of pectin did not cause damage to neutrophils and did not have direct pro- or antioxidant effects. However, when neutrophils were activated by mucin-treated microparticles, the incorporation of pectin into vaterite microspheres enabled an antioxidant effect to be reached, presumably via exposed ligands.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Eleonora A. Starikova, Jennet T. Mammedova, Arina Ozhiganova, Tatiana A. Leveshko, Aleksandra M. Lebedeva, Alexey V. Sokolov, Dmitry V. Isakov, Alena B. Karaseva, Larissa A. Burova, Igor V. Kudryavtsev
Summary: In this study, the impact and mechanism of streptococcal arginine deiminase (ADI) on the function of human peripheral blood T lymphocytes were investigated. The results showed that ADI decreased arginine concentration, suppressed immune cell activity and altered cell differentiation state, as well as inhibited autophagy processes.