期刊
JOURNAL OF CELL SCIENCE
卷 128, 期 11, 页码 2118-2129出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.166744
关键词
Non-coding RNA; Cell proliferation; DNA replication
类别
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/K013378/1]
- BBSRC doctoral training grant (DTG) [BB/F016581/1]
- BBSRC [BB/K013378/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [977500, BB/K013378/1] Funding Source: researchfish
Stem bulge RNAs (sbRNAs) are a family of small non-coding stem-loop RNAs present in Caenorhabditis elegans and other nematodes, the function of which is unknown. Here, we report the first functional characterisation of nematode sbRNAs. We demonstrate that sbRNAs from a range of nematode species are able to reconstitute the initiation of chromosomal DNA replication in the presence of replication proteins in vitro, and that conserved nucleotide sequence motifs are essential for this function. By functionally inactivating sbRNAs with antisense morpholino oligonucleotides, we show that sbRNAs are required for S phase progression, early embryonic development and the viability of C. elegans in vivo. Thus, we demonstrate a new and essential role for sbRNAs during the early development of C. elegans. sbRNAs show limited nucleotide sequence similarity to vertebrate Y RNAs, which are also essential for the initiation of DNA replication. Our results therefore establish that the essential function of small non-coding stem-loop RNAs during DNA replication extends beyond vertebrates.
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