Review
Cell Biology
Sharon R. Garrott, John P. Gillies, Morgan E. DeSantis
Summary: Cytoplasmic dynein-1 is a crucial microtubule motor that regulates various cellular functions. While the mechanisms of many of its regulators have been studied, the details of how Nde1 and Ndel1 interact with dynein remain unknown.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kyoko Okada, Bharat R. Iyer, Lindsay G. Lammers, Pedro A. Gutierrez, Wenzhe Li, Steven M. Markus, Richard J. Mckenney
Summary: This study reveals previously unknown steps in the activation pathway of cytoplasmic dynein-mediated cargo transport and provides insight into the integrated activities of LIS1/Ndel1 and dynactin/cargo-adapters. The findings contribute to the understanding of the roles of the human disease genes Ndel1 and LIS1 in the assembly and activation of dynein transport complexes.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Wen Lu, Margot Lakonishok, Anna S. Serpinskaya, Vladimir Gelfand
Summary: Cytoplasmic dynein plays essential roles in Drosophila oocyte growth by actively moving microtubules within nurse cells and from nurse cells to the oocyte via the cytoplasmic bridges, and this movement can transport cytoplasmic particles to the oocyte.
Article
Neurosciences
Jai P. Pandey, Liang Shi, Remi A. Brebion, Deanna S. Smith
Summary: Defective mitochondrial dynamics in axons are associated with developmental and late-onset neurological disorders. The regulatory pathway between LIS1 and dynein may play an important role in retrograde transport of mitochondria.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Microbiology
Jing Xu, Jie Gao, Maolin Zhang, Danwei Zhang, Ming Duan, Zhenhong Guan, Yidi Guo
Summary: Rabies virus infects N2a cells in a manner dependent on microtubule integrity as well as the function of dynein and kinesin. Microtubule-depolymerizing drugs, dynein inhibitors, and disruption of kinesin function can all hinder Rabies virus infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Neurosciences
Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, Tuanlao Wang, Bettina Winckler
Summary: In all cell types, endocytosed cargo is transported along a set of endosomal compartments, from early endosomes (EEs) via late endosomes (LEs) to lysosomes which are critical for degradation of proteins. Rab7 plays a key role in the maturation, motility, and fusion of endosomes with lysosomes. The study demonstrates that dynein is responsible for the retrograde transport of late endosomes in dendrites.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Cell Biology
Bhaba K. Das, Jyoti Gogoi, Aarthi Kannan, Ling Gao, Weirong Xing, Subburaman Mohan, Haibo Zhao
Summary: Cytoskeleton organization and lysosome secretion are crucial in osteoclastogenesis and bone resorption. Here, the role of NDE1 and NDEL1 in osteoclast biology was studied. The results showed that NDE1 plays an important role in regulating osteoclastogenesis, while NDEL1 is not necessary for osteoclast differentiation and function.
Article
Neurosciences
Shatabdi Bhattacharjee, Erin N. N. Lottes, Sumit Nanda, Andre Golshir, Atit A. A. Patel, Giorgio A. A. Ascoli, Daniel N. N. Cox
Summary: This research revealed the role of PP2A phosphatase in regulating dendrite development in Drosophila neurons. The PP2A complex has cell-type specific regulatory effects and is involved in regulating cytoskeletal organization and organelle localization.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Alexander S. Zhovmer, Alexis Manning, Chynna Smith, James B. Hayes, Dylan T. Burnette, Jian Wang, Alexander X. Cartagena-Rivera, Nikolay V. Dokholyan, Rakesh K. Singh, Erdem D. Tabdanov
Summary: The study demonstrates an antagonistic mechanical balance within the dynein-kinesin microtubular motor system, where dynein activity drives inward compaction of the microtubular network, while kinesins bundle and expand MTs into giant circular bands. This balance controls the 3D architecture, mechanics, and cell-microenvironment interactions of cells through orthogonal mechanisms of MT network reorganization.
Article
Multidisciplinary Sciences
Han-Hsuan Liu, Chien-Hsiang Hsu, Lily Y. Jan, Yuh-Nung Jan
Summary: The study established a photo-switchable assay system for detecting dendrite degeneration and repair, and used the WldS protein as a test case to investigate the mechanisms underlying dendrite regeneration and repair.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Physiology
Thomas A. Jepps
Summary: This article summarizes the impact of the microtubule network on arterial contractility, emphasizing the importance of microtubules in vascular smooth muscle cells. Recent findings highlight the crucial role of microtubules in the trafficking of voltage-dependent Kv7 channels.
Article
Neurosciences
Ann-Kristin Hoersting, Dietmar Schmucker
Summary: Recent progress in human genetics and single cell sequencing has expanded our understanding of brain wiring, while studies on the developmental causes of psychiatric diseases have shown significant advancements, emphasizing the importance of axonal branching and synapse formation. These findings highlight the need for increased efforts in understanding the developmental mechanisms of circuit assembly and the impact of neuronal shape diversity on specificity.
CURRENT OPINION IN NEUROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yerbol Tagay, Sina Kheirabadi, Zaman Ataie, Rakesh K. Singh, Olivia Prince, Ashley Nguyen, Alexander S. Zhovmer, Xuefei Ma, Amir Sheikhi, Denis Tsygankov, Erdem D. Tabdanov
Summary: The principal cause of death in cancer patients is metastasis, which is conventionally linked to actomyosin-driven cell locomotion. However, this study identifies a complementary mechanism of metastatic locomotion powered by dynein-generated forces. These findings provide new insights into the fundamental understanding of cell locomotion mechanisms and expand the spectrum of clinical targets against metastasis.
Article
Immunology
Stephanie L. Silva-Del Toro, Lee-Ann H. Allen
Summary: Neutrophils, also known as PMNs, show heterogeneous characteristics and considerable plasticity, as demonstrated by the induction of N1-like subtype differentiation in H. pylori infection. The study revealed that microtubule dynamics and dynein activity are essential for the hypersegmentation of the nucleus, with an increase in microtubule abundance and length in hypersegmented cells. These findings advance the understanding of nuclear morphology regulation processes.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Thomas D. D. Cushion, Ines Leca, David A. A. Keays
Summary: Microtubules are essential for various cellular functions and mutations in tubulin genes can lead to a range of diseases collectively known as tubulinopathies. Recent studies have shown that these mutations also affect microtubule-associated proteins (MAPs), which play important roles in microtubule regulation. In this review, we analyze the disease mechanisms caused by tubulin mutations on MAP binding and discuss how genetic variation can be utilized to identify novel MAPs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Yiming Zheng, Vito Mennella, Steven Marks, Jill Wildonger, Esraa Elnagdi, David Agard, Timothy L. Megraw
MOLECULAR BIOLOGY OF THE CELL
(2016)
Article
Multidisciplinary Sciences
Karl J. Petersen, Holly V. Goodson, Ashley L. Arthur, G. W. Gant Luxton, Anne Houdusse, Margaret A. Titus
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
Michael Winding, Michael T. Kelliher, Wen Lu, Jill Wildonger, Vladimir I. Gelfand
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Cell Biology
Brian V. Jenkins, Harriet A. J. Saunders, Helena L. Record, Dena M. Johnson-Schlitz, Jill Wildonger
JOURNAL OF CELL SCIENCE
(2017)
Review
Genetics & Heredity
Ethan Bier, Melissa M. Harrison, Kate M. O'Connor-Giles, Jill Wildonger
Article
Cell Biology
Michael T. Kelliher, Yang Yue, Ashley Ng, Daichi Kamiyama, Bo Huang, Kristen J. Verhey, Jill Wildonger
JOURNAL OF CELL BIOLOGY
(2018)
Review
Genetics & Heredity
Ethan Bier, Melissa M. Harrison, Kate M. O'Connor-Giles, Jill Wildonger
Article
Cell Biology
Connie Yan, Fei Wang, Yun Peng, Claire R. Williams, Brian Jenkins, Jill Wildonger, Hyeon-Jin Kim, Jonathan B. Perr, Joshua C. Vaughan, Megan E. Kern, Michael R. Falvo, E. Timothy O'Brien, Richard Superfine, John C. Tuthill, Yang Xiang, Stephen L. Rogers, Jay Z. Parrish
Article
Neurosciences
Michael T. Kelliher, Harriet A. J. Saunders, Jill Wildonger
CURRENT OPINION IN NEUROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Ashley L. Arthur, Livia D. Songster, Helena Sirkia, Akash Bhattacharya, Carlos Kikuti, Fernanda Pires Borrega, Anne Houdusse, Margaret A. Titus
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Courtney E. Coombes, Harriet A. J. Saunders, Anirudh G. Mannava, Dena M. Johnson-Schlitz, Taylor A. Reid, Sneha Parmar, Mark McClellan, Connie Yan, Stephen L. Rogers, Jay Z. Parrish, Michael Wagenbach, Linda Wordeman, Jill Wildonger, Melissa K. Gardner
Article
Genetics & Heredity
Sihui Z. Yang, Jill Wildonger
Article
Multidisciplinary Sciences
Ka Man Carmen Chan, Ashley L. Arthur, Johannes Morstein, Meiyan Jin, Abrar Bhat, Dorte Schlesinger, Sungmin Son, Donte A. Stevens, David G. Drubin, Daniel A. Fletcher
Summary: Fusion-associated small transmembrane (FAST) proteins are a diverse family of nonstructural viral proteins that drive cell-cell fusion, increasing viral spread and pathogenicity. Unlike viral fusogens with tall ectodomains, FAST proteins have short fusogenic ectodomains that cannot bridge the intermembrane gap between neighboring cells. This work suggests that localized mechanical pressure on the plasma membrane coupled to a membrane-disruptive ectodomain is sufficient to drive cell-cell fusion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Ashley L. Arthur, Amy Crawford, Anne Houdusse, Margaret A. Titus
Summary: This study reveals that filopodia initiation requires close collaboration between an actin-binding protein, the state of the actin cytoskeleton, and MF myosin activity.
Article
Biochemistry & Molecular Biology
Harriet A. J. Saunders, Dena M. Johnson-Schlitz, Brian Jenkins, Peter J. Volkert, Sihui Z. Yang, Jill Wildonger
Summary: Acetylation of microtubule protein K394 is crucial for synaptic morphogenesis and microtubule stability in the nervous system. The deacetylase HDAC6 regulates K394 acetylation and its overexpression leads to decreased microtubule stability.