期刊
JOURNAL OF CELL SCIENCE
卷 128, 期 6, 页码 1139-1149出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.163063
关键词
ADAM; Development; Neural crest; Wnt; Xenopus laevis
类别
资金
- National Institutes of Health USPHS [F31-DE023275, RO1-DE016289]
- Deutsche Forschungsgemeinschaft (DFG) [STE 613/8-2]
Cranial neural crest (CNC) cells are a transient population of stem cells that originate at the border of the neural plate and the epidermis, and migrate ventrally to contribute to most of the facial structures including bones, cartilage, muscles and ganglia. ADAM13 is a cell surface metalloprotease that is essential for CNC cell migration. Here, we show in Xenopus laevis embryos that the Wnt receptor Fz4 binds to the cysteine-rich domain of ADAM13 and negatively regulates its proteolytic activity in vivo. Gain of Fz4 function inhibits CNC cell migration and can be rescued by gain of ADAM13 function. Loss of Fz4 function also inhibits CNC cell migration and induces a reduction of mature ADAM13, together with an increase in the ADAM13 cytoplasmic fragment that is known to translocate into the nucleus to regulate gene expression. We propose that Fz4 associates with ADAM13 during its transport to the plasma membrane to regulate its proteolytic activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据