期刊
JOURNAL OF CELL SCIENCE
卷 128, 期 18, 页码 3466-3477出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.173013
关键词
Nuclear envelope formation; Nuclear pore complex; Mitotic exit; Lysine (K)-specific demethylase 1A; KDM1A; Histone modification; MEL28; ELYS; POM121; NDC1
类别
资金
- Max Planck Society
- European Research Council (ERC) [309528 CHROMDECON]
The metazoan nucleus breaks down and reassembles during each cell division. Upon mitotic exit, the successful reestablishment of an interphase nucleus requires the coordinated reorganization of chromatin and formation of a functional nuclear envelope. Here, we report that the histone demethylase LSD1 (also known as KDM1A) plays a crucial role in nuclear assembly at the end of mitosis. Downregulation of LSD1 in cells extends telophase and impairs nuclear pore complex assembly. In vitro, LSD1 demethylase activity is required for the recruitment of MEL28 (also known as ELYS and AHCTF1) and nuclear envelope precursor vesicles to chromatin, crucial steps in nuclear reassembly. Accordingly, the formation of a closed nuclear envelope and nuclear pore complex assembly are impaired upon depletion of LSD1 or inhibition of its activity. Our results identify histone demethylation by LSD1 as a new regulatory mechanism linking the chromatin state and nuclear envelope formation at the end of mitosis.
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