Article
Food Science & Technology
Sucheewin Krobthong, Yodying Yingchutrakul, Patompon Wongtrakoongate, Hathaichanok Chuntakaruk, Thanyada Rungrotmongkol, Chartchai Chaichana, Thanisorn Mahatnirunkul, Thitikorn Chomtong, Kiattawee Choowongkomon, Chanat Aonbangkhen
Summary: Obesity is a global health concern that can be prevented by physical activities and consuming nutrient-rich functional foods. In this study, nano-liposomal encapsulated bioactive peptides (BPs) were developed to reduce cellular lipids. The nano-liposomal BPs showed potential as functional food ingredients for preventing obesity.
Article
Pharmacology & Pharmacy
Jessica Nascimento, Isadora do Canto Olegario, Camila Mariot, Thayse Viana de Oliveira, Paula dos Santos Chaves, Rafaela Oliveira, Edilene Gadelha de Oliveira, Silvia Staniscuaski Guterres, Andreia Buffon, Diogo Andre Pilger, Ruy Carlos Ruver Beck
Summary: In this study, the encapsulation of orlistat (ORL) in polymeric aqueous nanocapsules (NC) was evaluated for its in vitro cytotoxic activity against cervical cancer cells. The nanoencapsulation improved the cytotoxic effect of ORL, suggesting its potential as a treatment for cervical cancer.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jessica Nascimento, Camila Mariot, Debora R. B. Vianna, Lucia M. Kliemann, Paula S. Chaves, Massimo Loda, Andreia Buffon, Ruy C. R. Beck, Diogo A. Pilger
Summary: This study investigated the expression of Fatty acid synthase (FASN) in premalignant lesions and cervical cancer samples, as well as the effects of a FASN inhibitor on cervical cancer cells. The results showed that FASN was expressed in all cervical cancer samples and its expression increased with advanced stages of cervical cancer. The FASN inhibitor orlistat reduced the viability of cervical cancer cells in a time-dependent manner and induced apoptosis, cell cycle arrest, and autophagy.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Cell Biology
Wenting Dai, Zhichao Wang, Guan Wang, Qiong A. Wang, Ralph DeBerardinis, Lei Jiang
Summary: Fatty acid synthase is essential for lipogenesis in cancer cells, but anchorage-independent malignant cells can enhance their redox capacity through reductive carboxylation.
Article
Dentistry, Oral Surgery & Medicine
Luciana Yamamoto de Almeida, Fernanda Dos Santos Moreira, Guilherme Augusto Silva Dos Santos, Florence Juana Maria Cuadra Zelaya, Cesar Alexander Ortiz, Michelle Agostini, Flavia Sammartino Mariano, Debora Campanella Bastos, Ulisses Ribaldo Nicolau Daher, Luiz Paulo Kowalski, Ricardo D. Coletta, Edgard Graner
Summary: The study found that orlistat can increase the sensitivity of SCC-9 LN-1 cells to cisplatin and paclitaxel chemotherapy drugs by downregulating the levels of Cyclin B1. However, the inhibitory effects on proliferation, migration, and invasion of SCC-9 LN-1 cells induced by orlistat in combination with cisplatin or paclitaxel were not significantly superior to using chemotherapy drugs alone.
Article
Pharmacology & Pharmacy
Jovana Jovankic, Danijela D. Nikodijevic, Milena G. Milutinovic, Aleksandra G. Nikezic, Vesna V. Kojic, Aleksandar M. Cvetkovic, Danijela M. Cvetkovic
Summary: Breast cancer, the second leading cause of death worldwide, has prompted research into non-standard drugs for suppressing its progression. The anti-obesity drug Orlistat has shown significant effects on cancer cell metabolism, such as antiproliferative, proapoptotic, antiangiogenic, antimetastatic, and hypolipidemic effects. Evaluations of Orlistat's anticancer potential in breast cancer cells MDA-MB-231, MDA-MB-468, and MCF-7 showed significant cytotoxic, proapoptotic, and anti-angiogenic effects, while having no significant cytotoxic effects on normal cells. Orlistat also affected lipid levels and redox status parameters, and suppressed genes and proteins involved in fatty acid synthesis, making it a promising supplement for antiangiogenic therapy against breast cancer without side effects.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pei-Wei Shueng, Hui-Wen Chan, Wei-Chan Lin, Deng-Yu Kuo, Hui-Yen Chuang
Summary: Sorafenib is an effective drug for hepatocellular carcinoma, but resistance often occurs. This study found that resistance to sorafenib could be reversed by inhibiting fatty acid synthesis using orlistat, and this combination treatment enhanced cell killing in hepatocellular carcinoma by altering cell metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Jennifer R. Landes, Brooke R. Bartley, Stephen A. Moore, Qin He, Rebecca Simonette, Peter L. Rady, Hung Q. Doan, Stephen K. Tyring
Summary: This study demonstrates that Selinexor may provide clinical benefit for patients with metastatic MCC refractory to immune checkpoint inhibitors through inhibition of the lipogenesis pathway. The findings further elucidate Selinexor's mechanism against MCC growth and metastasis.
CLINICAL AND EXPERIMENTAL DERMATOLOGY
(2023)
Article
Endocrinology & Metabolism
Yudai Uehira, Hiroaki Ueno, Junki Miyamoto, Ikuo Kimura, Yohei Ishizawa, Hiroshi Iijima, Shota Muroga, Toru Fujita, Soichi Sakai, Yoshishige Samukawa, Yuri Tanaka, Shinya Murayama, Hideyuki Sakoda, Masamitsu Nakazato
Summary: This study explored the effects of orlistat on the gut microbiota and fatty acid metabolism in Japanese individuals with obesity. The results showed that orlistat treatment led to significant reductions in body weight, waist circumference, and blood leptin concentrations. Furthermore, it was found that orlistat could alter the composition of gut microbiota and increase the levels of certain beneficial bacteria.
OBESITY RESEARCH & CLINICAL PRACTICE
(2023)
Article
Biochemistry & Molecular Biology
Ji Su Yang, Naeun Yoon, Mingyu Kong, Byung Hwa Jung, Hyunbeom Lee, Jinyoung Park
Summary: While attempts were made to inhibit cancer cell growth by combining FASN and USP14 inhibitors, there were no synergistic effects on cancer cell death compared to FASN inhibitor alone. Surprisingly, the USP14 inhibitor IU1 did not significantly affect FASN levels in cancer cells, and metabolite changes in IU1-treated cells differed significantly from those in cells treated with the FASN inhibitor Fasnall.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Given Lee, Sifei Han, Zijun Lu, Jiwon Hong, Anthony R. J. Phillips, John A. Windsor, Christopher J. H. Porter, Natalie L. Trevaskis
Summary: Orlistat is a pancreatic lipase inhibitor used to treat obesity by inhibiting dietary lipid absorption. Improved absorption could potentially treat other diseases as well.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Oncology
Chiao-Pei Cheng, Shu-Ting Liu, Yi-Lin Chiu, Shih-Ming Huang, Ching-Liang Ho
Summary: The study shows that overexpression of USP2a leads to stabilization of FAS protein, and inhibiting USP2a activity can increase FAS protein degradation, highlighting the importance of finding inhibitors of USP2a. 6-Thioguanine and 6-Mercaptopurine can decrease levels of USP2a target proteins FAS and Mdm2, and affect cell cycle proteins to regulate the cell cycle.
FRONTIERS IN ONCOLOGY
(2021)
Article
Food Science & Technology
Wenyuan Huang, Xing Guo, Chunyan Wang, Amantay Alzhan, Zhengan Liu, Xiaofeng Ma, Qingyan Shu
Summary: This study found that alpha-linolenic acid (ALA) inhibits the fatty acid synthesis pathway in breast cancer cells, inducing cell apoptosis and inhibiting invasion, metastasis, and cell cycle progression.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemical Research Methods
Baiyi Quan, Morgan A. Bailey, John Mantyh, David S. Hsu, Michael C. Fitzgerald
Summary: In this study, three protein folding stability profiling techniques were used to identify differentially stabilized proteins in colorectal cancer cell lines with different oxaliplatin sensitivities. The techniques successfully identified unique and novel proteins, including 36 proteins that were differentially stabilized in both cell line and patient-derived xenograft studies. Two of these proteins, fatty acid synthase and elongation factor 2, were validated and found to be potential drug targets for improving the efficacy of CRC chemotherapy. These findings enhance our understanding of CRC oxaliplatin resistance and provide insights for predicting sensitivity and overcoming chemoresistance in CRC.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
James Drury, Lyndsay E. A. Young, Timothy L. Scott, Courtney O. Kelson, Daheng He, Jinpeng Liu, Yuanyan Wu, Chi Wang, Heidi L. Weiss, Teresa Fan, Matthew S. Gentry, Ramon Sun, Yekaterina Y. Zaytseva
Summary: Altered lipid metabolism, specifically the overexpression of Fatty Acid Synthase (FASN), plays a significant role in colorectal cancer (CRC) progression. The study demonstrates that reducing FASN expression increases mouse survival and reduces the number of intestinal adenomas in a mouse model. Furthermore, downregulation of FASN expression inhibits pathways involved in cellular proliferation, energy production, and CRC progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Weizhuo Lu, Zhiwu Chen, Jiyue Wen
Summary: Ischemic stroke is a common and serious disease, and neuroinflammation plays a crucial role in its progression. Microglia, astrocytes, and infiltrating immune cells are involved in the complicated neuroinflammation cascade, releasing different molecules that affect inflammation. Flavonoids, plant-specific compounds, have shown protective effects against cerebral ischemia injury by modulating the inflammatory responses.
BIOMEDICINE & PHARMACOTHERAPY
(2024)
