Article
Chemistry, Analytical
Chia-Wen Chang, Yu-Hsuan Lin, Cheng-Han Tsai, Sivasankar Kulandaivel, Yi-Chun Yeh
Summary: A novel heat-stable enzyme was developed for a protein-and cell-based biosensor, enabling the detection of L-DOPA for the first time. The detection of L-DOPA was achieved through a red fluorescent signal within 30 minutes. Furthermore, a sequential analysis method using dual-fluorescent signals was developed for the sensitive detection of dopamine and L-DOPA coexistence, allowing for monitoring of the therapy for Parkinson's disease patients treated with L-DOPA.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Thales Henrique Cherubino Ribeiro, Raphael Ricon de Oliveira, Tais Teixeira das Neves, Wilder Douglas Santiago, Bethania Leite Mansur, Adelir Aparecida Saczk, Mario Lucio Vilela de Resende, Antonio Chalfun-Junior
Summary: Using transcriptomic data, we investigated the plant metabolomes of Coffea and discovered several potentially valuable compounds. We analyzed RNAseq libraries from leaves exposed to various environmental conditions and created models of metabolic networks. Through LC-MS/MS analysis, we identified pathways responsible for producing important compounds, including L-DOPA, a medication used for Parkinson's disease. Coffee plants are a natural source of L-DOPA and other bioactive compounds with potential medicinal applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Dario Breitel, Paul Brett, Saleh Alseekh, Alisdair R. Fernie, Eugenio Butelli, Cathie Martin
Summary: The study successfully engineered tomato fruit enriched in L-DOPA through overexpression of BvCYP76AD6, and further increased L-DOPA levels by elevating the expression of the metabolic master regulator MYB12. The research also revealed new roles for L-DOPA in plants, impacting fruit quality parameters and providing opportunities for the development of new biological sources of L-DOPA.
METABOLIC ENGINEERING
(2021)
Article
Neurosciences
Agustina Zorgniotti, Yanina Ditamo, Carlos A. Arce, C. Gaston Bisig
Summary: The study found that L-dopa irreversibly incorporates into tubulin, alters mitochondrial transport along axons, and reduces the affinity of the molecular motor KIF5B for Dopatubulin-containing microtubules. This disruption in microtubule functioning impairs mitochondrial traffic, which may have implications for Parkinson's disease patients receiving chronic L-dopa treatment.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Jael R. Neyra Recky, Mariana P. Serrano, M. Laura Dantola, Carolina Lorente
Summary: Tyrosine, an amino acid, plays a crucial role in physiological events, and its oxidation can have both beneficial and detrimental effects on biological systems. The degradation of tyrosine often begins with the loss of an electron in the presence of an electron acceptor, and DOPA is identified as a key product in this process, showing antioxidant properties.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Agronomy
Shucheng Duan, Soon Jae Kwon, You Jin Lim, Chan Saem Gil, Chengwu Jin, Seok Hyun Eom
Summary: L-dopa-rich faba tissues are a potential source for pharmaceutical purposes, mainly accumulating in germinating sprouts and flowers, while having lower contents in leaves and pods.
Article
Geriatrics & Gerontology
Hiromi Sano, Atsushi Nambu
Summary: ZNS is an anticonvulsant drug that has beneficial effects on Parkinson's disease and its side effect, L-DOPA-induced dyskinesia, but can also have adverse effects.
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Eldin Kurpejovic, Volker F. Wendisch, Berna Sariyar Akbulut
Summary: The increase in elderly patients with Parkinson's disease is driving market growth for l-DOPA, with biotechnological approaches in Corynebacterium glutamicum cells showing potential for economically feasible production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
M. Alejandra Guerrero-Rubio, Nathanael Walker-Hale, Rui Guo, Hester Sheehan, Alfonso Timoneda, Fernando Gandia-Herrero, Samuel F. Brockington
Summary: This study revisited a publication by Bean et al. (2018) on the evolution of l-DOPA 4,5-dioxygenase (DODA) activity in Caryophyllales. The authors replicated the analyses conducted by Bean et al. (2018) and found additional residues that were implicated in the activity of BvDODAa1. Their in vivo and in vitro assays showed that the original seven residue substitutions identified by Bean et al. (2018) had minimal effect on the catalytic activity of BvDODAa2. The authors concluded that the evolutionary pathway to high DODA activity is more complex than previously thought.
Article
Chemistry, Multidisciplinary
Rima, Safri Ishmayana, Desak Made Malini, Ukun M. S. Soedjanaatmadja
Summary: Mucuna pruriens (L.) DC (Velvet beans) is a tropical bean that contains a significant amount of L-Dopa and has been widely used as a natural remedy for Parkinson's disease. This study investigated the nutritional content of M. pruriens seeds and isolated L-Dopa, and evaluated its toxicity, antibacterial activity, antioxidant activity, and anti-Parkinson's activity.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Dong Du, Yingli Su, Qi Shang, Chan Chen, Weikang Tang, Ling Zhang, Haisheng Ren, Wenbin Liu
Summary: This study developed a biomimetic system for the production of L-DOPA from tyrosine with high efficiency and selectivity. By using tyrosine as substrate and a catalyst to simulate the active center of tyrosine hydroxylase, L-DOPA was successfully synthesized under optimized process parameters.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Agronomy
Er-Hu Chen, Qiu-Li Hou, Wei Dou, Pei-Jin Yang, Jin-Jun Wang
Summary: This study identified 24 tyrosine metabolism pathway genes in the genome of the oriental fruit fly Bactrocera dorsalis and established gene expression profiles during 15 developmental stages. Thirteen enzyme genes were found to be involved in pupariation, with the DDC enzyme playing a crucial role in larval-pupal tanning of B. dorsalis.
PEST MANAGEMENT SCIENCE
(2022)
Article
Chemistry, Analytical
Kantima Kaewjua, Weena Siangproh
Summary: This study aimed to develop an innovative and simple electrochemical sensor for melanoma and related disorders using the simultaneous determination of l-DOPA and l-Tyr. The sensor was fabricated by layer-by-layer electrodeposition of nanodiamond and poly L-proline onto a graphene electrode. The modified electrodes showed improved sensitivity and the ability to exclude interfering substances. The sensor demonstrated good performance in the detection of l-DOPA and l-Tyr in human urine and blood serum samples.
Article
Oncology
Kun Chang, Jiaqi Su, Chuanyu Li, Aihetaimujiang Anwaier, Wangrui Liu, Wenhao Xu, Yuanyuan Qu, Hailiang Zhang, Dingwei Ye
Summary: This study demonstrates that decreased DDC expression is significantly associated with poor survival and an immune-suppressive tumor microenvironment in ccRCC. The findings suggest that DDC could serve as a biomarker for guiding molecular diagnosis and developing novel therapeutic strategies for advanced ccRCC patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Hiraku Uchimura, Kaori Kanai, Masami Arai, Miyu Inoue, Akitoyo Hishimoto, Daiki Masukawa, Yoshio Goshima
Summary: Methylphenidate (MPH) and methamphetamine (METH) are commonly used for treating attention deficit/hyperactivity disorder. MPH increases dopamine (DA) release but has no effect on L-DOPA release, while METH induces the release of both DA and L-DOPA. However, MPH-induced hyperlocomotion and rewarding effects are reduced in mice lacking the Gpr143 (LDOPA receptor) gene. These findings indicate the involvement of GPR143 in the actions of MPH.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2023)
Article
Medical Laboratory Technology
Danilo Villalta, Andrea Seaman, Marychel Tiongson, Charles Warren, Chelsea Bentow, Nicola Bizzaro, Maria Grazia Alessio, Brunetta Porcelli, Gary L. Norman, Michael Mahler
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2020)
Article
Multidisciplinary Sciences
Nicola Bizzaro, Giampaola Pesce, Maria Teresa Trevisan, Manuela Marchiano, Luigi Cinquanta, Maria Infantino, Giusy Paura, Marilina Tampoia, Maria Grazia Alessio, Giulia Previtali, Magda Marchese, Clelia Zullo, Danilo Villalta, Ignazio Brusca, Mario Laneve, Caterina Castiglione, Teresa Carbone, Carmela Curcio, Laura Invernizzi, Fabrizio Montecucco, Daniele Saverino, Fabio Ferretti, Brunetta Porcelli
SCIENTIFIC REPORTS
(2020)
Review
Immunology
Maria Infantino, Teresa Carbone, Mariangela Manfredi, Valentina Grossi, Antonio Antico, Maria Piera Panozzo, Ignazio Brusca, Maria Grazia Alessio, Giulia Previtali, Stefan Platzgummer, Luigi Cinquanta, Giusy Paura, Gaia Deleonardi, Maria Teresa Trevisan, Antonella Radice, Caterina Castiglione, Tiziana Imbastaro, Martina Fabris, Giampaola Pesce, Brunetta Porcelli, Lucia Terzuoli, Maria Concetta Sorrentino, Marilina Tampoia, Letizia Abbracciavento, Danilo Villalta, Mariaelisabetta Conte, Giuseppina Barberio, Nicoletta Gallo, Maurizio Benucci, Nicola Bizzaro
AUTOIMMUNITY REVIEWS
(2020)
Letter
Pharmacology & Pharmacy
Antonella Fioravanti, Brunetta Porcelli, Lucia Terzuoli, Maria Romana Bacarelli, Sara Tenti, Sara Cheleschi
CLINICAL DRUG INVESTIGATION
(2020)
Article
Biochemical Research Methods
Maria Infantino, Mario Merone, Mariangela Manfredi, Valentina Grossi, Alessandra Landini, Maria Grazia Alessio, Giulia Previtali, Maria Teresa Trevisan, Brunetta Porcelli, Martina Fabris, Donatella Macchia, Danilo Villalta, Luigi Cinquanta, Giulio Iannello, Paolo Soda, Nicola Bizzaro, D. Federico
Summary: This study aimed to investigate the impact of variation in analytical specificity among different anti-tTG methods on the detection of CD in cases with anti-tTG positive/EMA negative results. The findings suggest that CD diagnosis is more likely related to the positivity of multiple anti-tTG IgA assays, rather than the levels of anti-tTG IgA antibodies. Results that are anti-tTG positive/EMA negative should be approached with caution, and laboratory comments should be provided, recommending referral to a CD reference center for further evaluation.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Review
Gastroenterology & Hepatology
Marco Di Tola, Nicola Bizzaro, Mariarosa Gaudio, Carlotta Maida, Danilo Villalta, Maria Grazia Alessio, Giulia Previtali, Martina Fabris, Gaia Deleonardi, Marilina Tampoia, Ignazio Brusca, Giampaola Pesce, Brunetta Porcelli, Mariangela Manfredi, Maria Infantino
Summary: The association between celiac disease (CD) and selective immunoglobulin A deficiency (SIgAD) has been known for over fifty years, but diagnosing and monitoring patients with both conditions remain challenging. The 2012 ESPGHAN guidelines provided criteria for diagnosing CD in SIgAD patients, but issues such as selecting patients for specific IgG antibody testing and choosing reliable tests for diagnosis and follow-up still need to be addressed.
DIGESTIVE DISEASES AND SCIENCES
(2021)
Article
Endocrinology & Metabolism
Tania Pilli, Gilda Dalmazio, Brunetta Porcelli, Silvia Cantara, Antonella Tabucchi, Alessandro Pini, Adriano Spreafico, Alessandra Cartocci, Raffaella Forleo, Furio Pacini, Carlo Scapellato, Maria Grazia Castagna
Summary: This study aimed to investigate the prevalence and disease stage of autoimmune diseases in patients with ATD, identifying various organ-specific autoantibodies. The results showed that PCA and GADA were the most common autoantibodies, with all autoimmune diseases except celiac disease mainly at the potential stage. Sex, ATD type, smoking habit, and coexistence of other autoimmune diseases were associated with susceptibility to develop chronic atrophic gastritis or autoimmune diabetes mellitus.
Article
Biochemical Research Methods
Maria Infantino, Teresa Carbone, Ignazio Brusca, Maria-Grazia Alessio, Giulia Previtali, Stefan Platzgummer, Giusi Paura, Caterina Castiglione, Martina Fabris, Giampaola Pesce, Brunetta Porcelli, Lucia Terzuoli, Maria-Romana Bacarelli, Marilina Tampoia, Luigi Cinquanta, Danilo Villalta, Francesca Buzzolini, Boaz Palterer, Silvia Pancani, Maurizio Benucci, Mariangela Manfredi, Nicola Bizzaro
Summary: This study compared eight commonly used immunoassays for detecting anti-ENA antibodies and found that they have good consistency and comparability. Although the specificity of all methods was good, there were differences in analytical sensitivity.
JOURNAL OF IMMUNOLOGICAL METHODS
(2022)
Article
Immunology
Maria Infantino, Boaz Palterer, Giulia Previtali, Maria-Grazia Alessio, Danilo Villalta, Teresa Carbone, Stefan Platzgummer, Giusi Paura, Caterina Castiglione, Martina Fabris, Giampaola Pesce, Brunetta Porcelli, Lucia Terzuoli, Maria-Romana Bacarelli, Marilina Tampoia, Luigi Cinquanta, Ignazio Brusca, Francesca Buzzolini, Maurizio Benucci, Matteo Tortora, Lorenzo Tronchin, Valerio Guarrasi, Paolo Soda, Mariangela Manfredi, Nicola Bizzaro
Summary: This study shows that current technologies for detecting anti-double-stranded DNA antibodies are not fully comparable, especially in terms of their correlation with the Crithidia luciliae immunofluorescence test (CLIFT). The positioning of these methods in the diagnostic algorithm for systemic lupus erythematosus (SLE) is influenced by their different correlation with CLIFT.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2022)
Letter
Gastroenterology & Hepatology
Brunetta Porcelli, Nicola Bizzaro, Martina Fabris, Maria Teresa Trevisan, Salvatore S. Sciarrone, Stefano Brillanti
DIGESTIVE AND LIVER DISEASE
(2023)
Article
Immunology
Edoardo Conticini, Miriana d'Alessandro, Paolo Cameli, Laura Bergantini, Elena Pordon, Lucia Cassai, Luca Cantarini, Elena Bargagli, Bruno Frediani, Brunetta Porcelli
Summary: The study aimed to assess the prevalence of myositis-specific and myositis-associated antibodies (MSA and MAA) in patients previously diagnosed with non-specific interstitial pneumonia (NSIP) and without symptoms of idiopathic inflammatory myopathies (IIM). The results showed that 37.5% of the patients displayed significant MSA and/or MAA positivity, and two seropositive patients showed a significant impairment of FVC. A cautious assessment of NSIP patients, especially those with progressive and non-responsive disease, should include an evaluation of MSA and MAA.
IMMUNOLOGIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Brunetta Porcelli, Miriana d'Alessandro, Latika Gupta, Silvia Grazzini, Nila Volpi, Maria Romana Bacarelli, Federica Ginanneschi, Giovanni Biasi, Francesca Bellisai, Marta Fabbroni, David Bennett, Claudia Fabiani, Luca Cantarini, Elena Bargagli, Bruno Frediani, Edoardo Conticini
Summary: This study assessed the diagnostic accuracy of anti-cN1A antibodies in patients with suspected IIM and evaluated the agreement between line immunoassay (LIA) and enzyme-linked immunoassay (ELISA). The results showed excellent concordance between LIA and ELISA for detecting anti-cN1A antibodies, suggesting that LIA could be a rapid and useful adjunct, and it could even replace ELISA for cN1A assay.
Meeting Abstract
Rheumatology
Edoardo Conticini, Brunetta Porcelli, Nila Volpi, Maria Romana Bacarelli, Miriana d'Alessandro, Silvia Grazzini, Elena Bargagli, Luca Cantarini, Bruno Frediani
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Nicola Bizzaro, Martina Fabris, Brunetta Porcelli, Maria Teresa Trevisan, Salvatore S. Sciarrone, Eleonora Palella, Luisa Moser, Maria Grazia Alessio, Alessandra Romito, Mario Fracchia, Laura Sidoli, Giampaola Pesce, Sara Labanca, Andrea Costantini, Stefano Brillanti
Article
Urology & Nephrology
Brunetta Porcelli, Andrea Guarnieri, Fabio Ferretti, Guido Garosi, Lucia Terzuoli, Francesca Cinci, Antonella Tabucchi, Marilina Tampoia, Letizia Abbracciavento, Chiara Villani, Gaia Deleonardi, Ana Gabriela Grondona, Marcello Mazzolini, Gaetano La Manna, Marisa Santostefano, Maria Infantino, Mariangela Manfredi, Giuseppe Spatoliatore, Alberto Rosati, Chiara Somma, Nicola Bizzaro
Summary: Autoantibodies against-phospholipase A2 receptor (PLA2R) are specific markers of idiopathic membranous nephropathy (iMN), and commercial ELISA tests can effectively detect these antibodies with high sensitivity and specificity. The optimal cut-off value for positive detection was found to be 2.7 RU/ml, providing the best overall efficiency for the test.
JOURNAL OF NEPHROLOGY
(2021)
