Article
Oncology
Yibing Guan, Fangshi Xu, Juanhua Tian, Ke Gao, Ziyan Wan, Yiyuan Wang, Mei Gao, Zhenlong Wang, Tie Chong
Summary: Research shows that CTC-WBC clusters are related to prognosis in renal cell carcinoma patients. Total CTCs, pathology type, and CTC-WBC clusters can be used as prognostic indicators for MFS, while total CTCs and solid tumor diameter are prognostic indicators for OS.
Article
Oncology
Chunming Wang, Qiong Luo, Wenbin Huang, Cheng Zhang, Hangyu Liao, Kunling Chen, MingXin Pan
Summary: Liquid biopsy as a method for real-time monitoring of cancer patients is gaining attention. A new method combining CTC enrichment technology with NGS was explored to analyze genetic variations in hepatocellular carcinoma patients. The number of CTCs and CTC-WBC clusters in peripheral blood of patients with liver cancer was found to be related to specific genomic profiles.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Jing Chen, Yuhong Luo, Xiaoxue Xi, Haixia Li, Shufen Li, Lei Zheng, Dinghua Yang, Zhen Cai
Summary: This study explored the correlation between CTC-WBC clusters and clinicopathological characteristics in HCC patients and found that it was closely associated with microvascular invasion, portal vein tumor thrombus, and extrahepatic metastasis. The diagnostic performance of CTC-WBC clusters in discriminating metastatic HCC was higher than that of total CTCs and EMT-CTCs. Patients with positive CTC-WBC clusters had a higher relapse rate and shorter RFS. Dynamic monitoring of CTC-WBC clusters is an effective method for early detection and intervention of HCC recurrence and metastasis.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Justyna Topa, Peter Gresner, Anna J. Zaczek, Aleksandra Markiewicz
Summary: Circulating tumor cells (CTCs) are mediators of solid tumor dissemination and serve as early indicators of disease progression. This article provides a comprehensive review of the clinical significance of mesenchymal CTCs in breast cancer (BC) and analyzes various methods and markers used for CTC isolation. The results suggest that mesenchymal CTCs can be more efficiently captured using methods not solely based on epithelial enrichment.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Maria S. S. Tretyakova, Maxim E. E. Menyailo, Anastasia A. A. Schegoleva, Ustinia A. A. Bokova, Irina V. V. Larionova, Evgeny V. V. Denisov
Summary: The spread of tumor cells through the bloodstream is a critical step in cancer metastasis, but isolating circulating tumor cells (CTCs) is challenging due to their high heterogeneity and low representation in the blood. This review summarizes the available methods for isolating viable CTCs based on different cell properties and highlights the importance of CTC-derived models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Ying Wang, Yanxia Liu, Zhiyun Zhang, Baohua Lu, Yuan Gao, Li Tong, Mingming Hu, Peter Ping Lin, Baolan Li, Tongmei Zhang
Summary: This study examined the clinical utility of different types of aneuploid circulating tumor cells (CTCs) and specifically CTC-associated white blood cell (CTC-WBC) clusters in predicting treatment response, prognosis, and real-time monitoring of disease progression in advanced driver gene-negative non-small cell lung cancer (NSCLC) patients. The detection of CTC-WBC clusters in post-treatment patients was associated with reduced progression-free survival and overall survival, indicating a worse prognosis in both lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) patients.
Review
Pharmacology & Pharmacy
Zdenek Kejik, Robert Kaplanek, Petr Dytrych, Michal Masarik, Katerina Vesela, Nikita Abramenko, David Hoskovec, Martina Vasakova, Jarmila Kralova, Pavel Martasek, Milan Jakubek
Summary: Designing optimal adjuvant therapy is crucial in treating non-small-cell lung carcinoma. Standard methods may not be effective in cases with high level of CTCs, thus alternative therapeutic strategies like low-toxicity natural agents may be considered when standard methods fail. This review discusses NSCLC therapy strategies and potential options for treatment beyond standard methods.
Article
Oncology
Yujuan Zhang, Yu Men, Jianyang Wang, Puyuan Xing, Jun Zhao, Junling Li, Danfei Xu, Zhouguang Hui, Wei Cui
Summary: The study aimed to analyze the clinical relevance of heterogeneous phenotypes of peripheral circulating tumor cells (CTCs) in patients with non-small cell lung cancer (NSCLC). The results showed that pure hybrid epithelial/mesenchymal CTCs were more strongly associated with metastasis.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Pi Ding, Zhili Wang, Zeen Wu, Mingchao Hu, Weipei Zhu, Na Sun, Renjun Pei
Summary: A method using tannic acid-functionalized magnetic nanoparticles to efficiently isolate heterogeneous circulating tumor cells has been established, with successful validation in clinical blood samples from patients with various cancers.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Xinbang Jiang, Xiangyun Zhang, Chen Guo, Yameng Yu, Boya Ma, Zhuang Liu, Yamin Chai, Lichun Wang, Yunzheng Du, Biao Wang, Nan Li, Dong Dong, Yueguo Li, Xinglu Huang, Lailiang Ou
Summary: Immunomagnetic nanoparticles (IMNs) are a potential method for early cancer diagnosis, metastasis examination, and treatment guidance by isolating rare circulating tumor cells (CTCs) from whole blood. However, the formation of a protein corona on the nanoparticles reduces their performance in body fluids. To overcome this, a novel strategy was developed to precoat the protein corona onto magnetic nanoparticles (C-MNs) and conjugate their surfaces with an immuno-antibody, resulting in decreased protein adsorption and non-specific cell binding. The functionalized C-MNs (IC-MNs) maintained highly specific CTC capture performance when exposed to blood plasma, demonstrating their potential as a clinically applicable diagnostic tool.
Article
Oncology
Yaopeng Qiu, Xinxin Zhang, Xiangqian Deng, Renyi Zhang, Zhen Cai, Zhenzhan Zhang, Hao Liu
Summary: The presence of circulating tumor cell (CTC)-associated white blood cell clusters (CTC-WBC clusters) is associated with poor prognosis in patients with gastric cancer (GC) after radical gastrectomy.
Review
Multidisciplinary Sciences
Jon F. Edd, Avanish Mishra, Kyle C. Smith, Ravi Kapur, Shyamala Maheswaran, Daniel A. Haber, Mehmet Toner
Summary: Circulating tumor cells (CTCs), which enter the bloodstream from solid tumors, have the potential to initiate metastases. However, due to their extreme rarity and similarities to leukocytes, isolating CTCs from large blood volumes at high yield and purity remains a challenge. Recent advancements in microfluidics have allowed for high-throughput sorting of cells along multiple dimensions.
Article
Plant Sciences
Zu-Jun Que, Yun Yang, Hai-Tao Liu, Wen-Ji Shang-Guan, Pan Yu, Li-Hua Zhu, He-Gen Li, Huai-Min Liu, Jian-Hui Tian
Summary: The study aimed to investigate the anti-metastatic mechanism of Jinfukang in lung cancer by intervening in circulating tumor cells (CTCs). Results showed that Jinfukang inhibited the proliferation, migration, and invasion of CTCs, as well as inducing anoikis through suppression of the integrin/Src pathway. This suggests that Jinfukang may exert its anti-metastatic effects in lung cancer through anoikis.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Mert Boya, Tevhide Ozkaya-Ahmadov, Brandi E. Swain, Chia-Heng Chu, Norh Asmare, Ozgun Civelekoglu, Ruxiu Liu, Dohwan Lee, Sherry Tobia, Shweta Biliya, L. DeEtte McDonald, Bassel Nazha, Omer Kucuk, Martin G. Sanda, Benedict B. Benigno, Carlos S. Moreno, Mehmet A. Bilen, John F. McDonald, A. Fatih Sarioglu
Summary: The authors introduce a new technology, Cluster-Wells, which enables fast and accurate isolation and analysis of CTC clusters in blood, providing a potential tool for further studying the utility of these clusters in cancer management.
NATURE COMMUNICATIONS
(2022)
Review
Oncology
Francesca Chemi, Sumitra Mohan, Tatiana Guevara, Alexandra Clipson, Dominic G. Rothwell, Caroline Dive
Summary: Circulating tumor cells play a causal role in cancer metastasis, and advancements in cellular and molecular technologies have improved understanding of their characteristics. Despite challenges with low abundance, CTC enumeration is widely used for prognostic monitoring.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Sajay Bhuvanendran Nair Gourikutty, Chang Chia-Pin, Daniel Puiu Poenar
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2016)
Article
Biochemical Research Methods
Sajay Bhuvanendran Nair Gourikutty, Chia-Pin Chang, Poenar Daniel Puiu
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2016)
Article
Engineering, Biomedical
Bhuvanendran Nair Gourikutty Sajay, Chia-Pin Chang, Hamizah Ahmad, Puttachat Khuntontong, Chee Chung Wong, Zhiping Wang, Poenar Daniel Puiu, Ross Soo, Abdur Rub Abdur Rahman
BIOMEDICAL MICRODEVICES
(2014)
Article
Multidisciplinary Sciences
Hamizah Ahmad Cognart, Jean-Louis Viovy, Catherine Villard
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Yaelim Lee, Megan Finch-Edmondson, Hamizah Cognart, Bowen Zhu, Haiwei Song, Boon Chuan Low, Marius Sudol
Article
Biology
Jason K. H. Lai, Pearlyn J. Y. Toh, Hamizah A. Cognart, Geetika Chouhan, Timothy E. Saunders
Summary: This study reveals that DNA damage induced by Yap1 and Wwtr1 mutations in zebrafish can lead to apoptosis of epidermal basal cells, and that cell size plays a critical role in this process.
Article
Engineering, Electrical & Electronic
Bhuvanendran Nair Gourikutty Sajay, Chiam Su Yin, Qasem Ramadan
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2017)
Article
Engineering, Biomedical
Yunxiao Liu, Patthara Kongsuphol, Sajay Bhuvanendran Nair Gourikutty, Qasem Ramadan
BIOMEDICAL MICRODEVICES
(2017)