Article
Biophysics
Dongli Sun, Tingting Fan, Feng Liu, Fangxiu Wang, Dan Gao, Jin-Ming Lin
Summary: A microfluidic chemiluminescence biosensor based on a multiple signal amplification strategy was developed for rapid and ultrasensitive detection of E. coli O157:H7. The biosensor showed excellent recovery rate and selectivity, making it a promising tool for rapid detection of foodborne pathogens.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Environmental
Shu Xiao, Cong Cao, Tinghong Ming, Yuting Cao, Zhenzhong Yu, Ning Gan
Summary: To solve the challenge of simultaneous detection of live and dead bacteria in food safety, researchers developed an all-in-one biosensor using a phage-apoferritin@CuO2 probe on an antimicrobial peptide (AMP)/MXenes-modified detection platform. The biosensor can simultaneously measure live and dead bacteria of Escherichia coli O157:H7 through bioluminescent, colorimetric, and electrochemical signals. The biosensor was successfully used for on-site measurement of live and dead E. coli O157:H7 in food samples with a detection limit of 30 CFU/mL for live bacteria and 6 CFU/mL for total bacteria within 50 min. This work presents a novel pathway for rapid and simultaneous quantification of both live and dead bacteria.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Bo Song, Jiayuan Yu, Yan Sun, Qiao Wang, Shengnan Xu, Yichen Jia, Shuying Lin, Yueying Zhang, Chen Wang, Yingbo Zhang, Xiaojie Zhang
Summary: This study developed a novel method for rapidly detecting E. coli on a microfluidic platform. By using the ATP method to determine bacterial concentration in samples and the immune-microsphere technique on a microfluidic chip, specific detection of E. coli was achieved. The method showed high sensitivity, short testing time, low cost, and great application value in medical diagnostics for bacterial detection.
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Smriti Shringi, Haiqing Sheng, Andrew A. Potter, Scott A. Minnich, Carolyn J. Hovde, Thomas E. Besser
Summary: Oral immunization with live stx-negative LEE+ E. coli O157:H7 in cattle reduces the colonization of wild-type E. coli O157:H7 strains at the rectoanal junction and shortens the shedding duration.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Ting Zheng, Xianming Li, Ya-Ni Xie, Bin Yang, Peng Wu
Summary: This study proposes a dual-gene RPA-LFA method to selectively detect E. coli O157:H7 by recognizing target amplicons and eliminating false positives. The assay shows high sensitivity and specificity for the detection of this pathogen.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Man-Seok Choi, Eun Bi Jeon, Ji Yoon Kim, Eun Ha Choi, Jun Sup Lim, Jinsung Choi, Shin Young Park
Summary: The efficacy of dielectric barrier discharge (DBD) plasma treatment for reducing non-pathogenic Escherichia coli and E. coli O157:H7 in oysters was studied. Exposure to DBD plasma for at least 60 minutes resulted in significant reductions in both types of bacteria, with minimal impact on the glycogen content and texture of the oysters. This non-thermal intervention could be a promising method for improving the safety of fresh oysters.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Martha Sanchez-Tamayo, Blanca Ruiz-Llacsahuanga, Rawane Raad, William Kerr, Faith Critzer
Summary: This study evaluated the efficacy of incorporating natural antimicrobials into carnauba wax and applying it to apples. The results showed that carnauba wax containing 2% essential oils was the most effective treatment for inactivating bacterial foodborne pathogens on apples.
Article
Chemistry, Analytical
Nilamben Panchal, Vedant Jain, Rebekah Elliott, Zachary Flint, Paul Worsley, Caine Duran, Tuhina Banerjee, Santimukul Santra
Summary: Increasing foodborne illnesses have created global health and economic burdens, necessitating the development of specific and rapid testing methods for identifying foodborne pathogens. This study introduces a nanoplatform based on nanozymes that can efficiently and accurately identify pathogens in the agrifood industry.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Ilaria Berruti, Isabel Oller, Maria Inmaculada Polo-Lopez
Summary: This study investigated the effectiveness of non-activated peroxymonosulfate as an oxidative agent for water purification, showing significant enhancement in bacterial and contaminant oxidation under solar irradiation. Testing under different water quality conditions revealed high chloride concentration had a positive effect, while complex water compositions reduced system efficiency.
Article
Chemistry, Analytical
Lu Zhao, Jianping Guo, Shijie Li, Junping Wang
Summary: A novel substoichiometric photothermal conversion material MoO3-x nanoparticles (4-MBA MoO3-x NPs) were successfully prepared and used to develop a thermal immunosensor for detecting the pathogen E. coli O157:H7. By utilizing immunomagnetic separation technology, the photothermal sensor probes coupled with antibodies enabled semi-quantitative and quantitative determination of E. coli O157:H7 with high efficiency and accuracy, showing great potential for practical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Meihong Cai, Gang Zhang, Changzhu Li, Xiaochen Chen, Haiying Cui, Lin Lin
Summary: This study introduced pomegranate peel polyphenols embedded in chitosan to prepare antibacterial nanofibers, showing effective inhibition of bacterial growth on food surfaces (e.g. E. coli) without affecting food quality. By adding different concentrations of polyphenols and polysaccharides, the properties of nanofibers can be controlled to enhance encapsulation rate and tensile strength.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Shuhong Zhang, Yuanbin Huang, Moutong Chen, Guangzhu Yang, Jumei Zhang, Qingping Wu, Juan Wang, Yu Ding, Qinghua Ye, Tao Lei, Yue Su, Rui Pang, Runshi Yang, Youxiong Zhang
Summary: This study systematically characterized E. coli O157:non-H7 strains isolated from retail food in Chinese markets. The strains displayed diverse H serotypes, virulence genes, and antibiotic resistance. Some strains also harbored plasmid-mediated colistin-resistant genes, which is a significant concern.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Chaiyong Wu, Dengfeng Li, Qianli Jiang, Ning Gan
Summary: A paper-based fluorescent phage biosensor was developed for the rapid and specific detection of virulent E. coli O157:H7 strain in food samples. The biosensor utilized a novel fluorescent probe and a micro-porous membrane filter to retain the targeted complex on a paper chip. The detection limit was as low as 50 CFU/mL, showing potential application for point-of-care testing of virulent E. coli in foods.
