Article
Green & Sustainable Science & Technology
Ahmed A. Ayash, Harith H. Al-Moameri, Ali Abed Salman, Arnold A. Lubguban, Roberto M. Malaluan
Summary: This study presents a novel microfluidic approach for complete separation of blood cells. The approach utilizes a polydimethylsiloxane serpentine microchannel with a series of electrodes, providing two separation zones. The design exploits the unique channel configuration and the inherent differences in dielectric properties of the three blood cell types to achieve size-based sorting. Platelets are separated in the first zone due to their larger dielectrophoretic force compared to red and white blood cells. The model developed in this work demonstrates the feasibility of the proposed approach, showing complete separation of blood content across different flow rates and AC frequencies.
Article
Mechanics
Naoki Takeishi, Hiroshi Yamashita, Toshihiro Omori, Naoto Yokoyama, Shigeo Wada, Masako Sugihara-Seki
Summary: This study numerically analyzed the lateral movement and equilibrium radial positions of red blood cells in a circular channel, revealing bistable flow modes (rolling and tumbling) with different equilibrium radial positions.
JOURNAL OF FLUID MECHANICS
(2022)
Article
Cell Biology
Brian S. Ferguson, Leslie E. Neidert, Matthew J. Rogatzki, Keith R. Lohse, L. Bruce Gladden, Heidi A. Kluess
Summary: The study found no significant difference in the release of ATP from RBCs of different densities under hypoxic and normoxic conditions, and the release of ATP was linearly correlated with the release of Hb.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Thermodynamics
Pankaj Srivastava, Ruchitkumar Ishwarlal Patel, Anupam Dewan
Summary: A comparative study was conducted on the flow and thermal characteristics in a double-layered microchannel with bifurcation, showing that the bifurcation plates reduce the overall thermal resistance but increase pressure drop and pumping power. Nusselt number is higher in parallel-flow than counter-flow, but the difference is greater at low Re values and diminishes with increasing flow rate. Counter-flow offers a more uniform temperature distribution essential for thermal system reliability.
INTERNATIONAL JOURNAL OF THERMAL SCIENCES
(2021)
Article
Oncology
Xinyan Geng, Jie Ma, Pushpa Dhilipkannah, Feng Jiang
Summary: In this study, microRNA profiling was performed on three major blood cell types to identify potential biomarkers for lung cancer. It was found that red blood cell-derived microRNAs could potentially serve as novel biomarkers, and integrating microRNAs from multiple cell sources yielded superior diagnostic accuracy for lung cancer.
Article
Chemistry, Analytical
Yang Jun Kang
Summary: In this study, three rheological properties (viscoelasticity, RBC aggregation, and blood junction pressure) were measured by analyzing blood velocity and image intensity in a microfluidic device. The analytical formula for viscoelasticity was derived based on the discrete fluidic circuit model. RBC aggregation was obtained by reducing blood velocity and analyzing microscopic image intensity. The blood junction pressure was obtained by integrating blood velocity within an air compliance unit.
Article
Biochemistry & Molecular Biology
Dania Fischer, Fabian Thies, Omar Awad, Camilla Brat, Patrick Meybohm, Patrick C. Baer, Markus M. Mueller, Anja Urbschat, Thorsten J. Maier, Kai Zacharowski, Jessica Roos
Summary: The relationship between allogeneic blood transfusions and increased rates of cancer recurrence, metastasis and death in colorectal cancer patients is still not fully understood. This study focused on the in vitro effects of microparticles derived from red blood cell units on colon carcinoma cells and found that these microparticles did not significantly affect cell characteristics or signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Nicola Conran, Stephen H. Embury
Summary: Sickle cell anemia is a hereditary hemoglobinopathy characterized by abnormal hemoglobin polymerization that triggers pathological mechanisms leading to various severe complications. Recent research has focused on the role of leukocytes in vaso-occlusive processes and the importance of adhesion by different cell types, including endothelial cells, platelets, leukocytes, and red blood cells.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Mechanics
Zhaomiao Liu, Zhaocheng Wang, Yan Pang, Peng Su, Xiang Wang
Summary: The dynamic behaviors of droplets transporting through the cross junction are studied experimentally and theoretically, focusing on the influences of initial droplet length, total flux, and flow rate combination of different inlets. More types of flow patterns and different thresholds of two behaviors (breakup and obstruction) under the influence of opposite flow are obtained. Deformation characteristics in different patterns and stages are discussed, and an empirical relation is proposed to estimate the time difference representing the unsynchronized necking. The importance of curvature component in the top view is proven during the breakup process, and two different mechanisms are proposed to explain the adjustable functions of the cross junction at different flow rate ratios.
Article
Engineering, Electrical & Electronic
Davoud Abbasinezhad Fallah, Mohammad Raad, Sajad Rezazadeh, Habib Jalili
Summary: This paper presents the design of a T-shape microchannel to enhance mixing quality, validated through numerical simulations and investigates the influence of the number of mixing unit periods and curvature on mixing effectiveness. The best mixing quality was achieved with 25-period mixing unit.
MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS
(2021)
Article
Mechanics
Akira Noguchi, Yuki Tange, Tomoaki Itano, Masako Sugihara-Seki
Summary: In blood flow through microvessels, platelets tend to concentrate near the vessel wall, but this distribution is disrupted and reconstructed downstream of a confluence. This study used in vitro experiments to investigate this redistribution distance using fluorescent particles as platelet surrogates and a Y-shaped confluence microchannel. The results showed that the redistribution of particles occurred within 5 mm from the confluence, which is comparable to or smaller than the redistribution distance in straight channels, but much smaller than after bifurcation.
FLUID DYNAMICS RESEARCH
(2023)
Article
Biochemical Research Methods
Tingting Chen, Licong Cui, Wang He, Renxing Liu, Chengqiang Feng, Lei Wu, Yang Wang, Huiyun Liu, Linmao Qian, Bingjun Yu
Summary: As important components in microfluidic chip analysis systems, micromixers have shown wide applications. However, due to fabrication limitations, it is still challenging to achieve high-quality micromixers with both well-designed structures and efficient mixing. This study proposes a method based on the theory of chaotic mixing to design and fabricate sinusoidal structures with variable phases. The results demonstrate that the scratched traces with phase differences can resist etching, leading to the generation of misaligned sine channels with alternating 3D shapes. Further analysis shows that reducing the line spacing in scratching can result in a thicker a-Si layer. With the proposed method, a misaligned sine micromixer with higher mixing efficiency is achieved. The study also investigates the duplicating process for high-precision production of micromixers. Overall, this research provides strategies for miniaturizing high-performance microfluidic chips.
Article
Multidisciplinary Sciences
Samuel Molcan, Monika Smieskova, Hynek Bachraty, Katarina Bachrata
Summary: In this study, a new method for estimating a key parameter of red blood cell elasticity using a trained neural network is proposed. The impact of different network architectures and data dimension reduction methods were investigated, providing recommendations for recording real experiments.
Article
Engineering, Biomedical
Yuanyuan Cheng, Qian Chen, Zhanyin Qian, Tianhe Shan, Liya Bai, Xiaoyu Jiang, Chunyu Li, Yinsong Wang
Summary: A versatile biomimetic system based on red blood cells (RBCs) is developed for comprehensive treatment of triple-negative breast cancer (TNBC). The system achieves long blood circulation, tumor vessel targeting, deep tissue penetration, and immune response induction through stepwise activation of nanoscale photothermal agents and photosensitizers, which effectively suppress TNBC recurrence and metastasis.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biophysics
Carlson Triebold, Jared Barber
Summary: Red blood cells make up a significant portion of blood and their distribution at vessel bifurcations is influenced by the endothelial surface layer. Research suggests that the presence of the endothelial glycocalyx layer can affect the distribution and function of red blood cells and may lead to pathological issues.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Biology
Azusa Kage, Toshihiro Omori, Kenji Kikuchi, Takuji Ishikawa
JOURNAL OF EXPERIMENTAL BIOLOGY
(2020)
Article
Gastroenterology & Hepatology
Kenji Kikuchi, Hyeongtak Noh, Keiko Numayama-Tsuruta, Takuji Ishikawa
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2020)
Article
Mechanics
Junichi Manabe, Toshihiro Omori, Takuji Ishikawa
JOURNAL OF FLUID MECHANICS
(2020)
Review
Cell Biology
Takuji Ishikawa, Hironori Ueno, Toshihiro Omori, Kenji Kikuchi
Summary: This article explores the roles of cilia and centrosomes in eukaryotic cells and their dynamics, motions, and forces at the molecular, organelle, and tissue levels. The structure and function of cilia and centrosomes are closely related, requiring a comprehensive understanding from the molecular level to the tissue level.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Satoshi Ii, Hiroki Kitade, Shunichi Ishida, Yohsuke Imai, Yoshiyuki Watanabe, Shigeo Wada
PLOS COMPUTATIONAL BIOLOGY
(2020)
Article
Physics, Fluids & Plasmas
Tomoki Oyama, Shunichi Ishida, Kohei Maeyama, Taimei Miyagawa, Yohsuke Imai
Summary: The study focuses on the effects of clustered contractions propagating along a circular channel on fluid flow, finding that clustered contractions cause retrograde flow and fluid trapping in the upstream and downstream regions of the cluster. Flow rate is not proportional to the number of peristaltic waves, particularly for large contraction ratios.
PHYSICAL REVIEW FLUIDS
(2021)
Article
Physics, Fluids & Plasmas
Shunichi Ishida, Ryota Matsumoto, Daiki Matsunaga, Yohsuke Imai
Summary: In this study, a lattice Boltzmann simulation was used to investigate the crystal-like structure of deformable particles in a wall-bounded shear flow. It was found that this crystal structure can be effectively utilized for the segregation of capsules of different sizes. The movement of the smaller capsules varied significantly with the size ratio, and their behavior changed from diffusive to advective depending on the ratio.
PHYSICAL REVIEW FLUIDS
(2022)
Article
Mechanics
Kohei Maeyama, Shunichi Ishida, Yohsuke Imai
Summary: Peristaltic pumping is the primary mechanism of food transport in the human intestine. This study investigates the peristaltic transport of a power-law fluid with a low behavior index, and finds that inertia effects can increase the discharge flow rate, while the sharpness of the contraction wave can affect the symmetry of the flow field. The results suggest that an isolated contraction wave with a shorter wavelength can transport intestinal contents more quickly when the contents have low consistency indices or when the contraction wave has a large propagation velocity.
Article
Multidisciplinary Sciences
Rika Ebara, Shunichi Ishida, Taimei Miyagawa, Yohsuke Imai
Summary: The amplitude and frequency of peristaltic contractions have a significant impact on gastric mixing and emptying. Increasing or decreasing the amplitude affects mixing strength and emptying rate, with higher amplitude increasing the emptying rate and lower amplitude decreasing it. The emptying rate also increases with the peristaltic frequency. The closest contraction wave to the pylorus and subsequent waves promote gastric emptying. It is important to maintain these parameters within normal ranges for healthy gastric function.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Multidisciplinary Sciences
Haruki Yoshida, Shunichi Ishida, Taiki Yamamoto, Takayuki Ishikawa, Yuichi Nagata, Kazuhito Takeuchi, Hironori Ueno, Yohsuke Imai
Summary: In this study, computational fluid dynamics was used to investigate the effects of cilia-induced surface velocity on cerebrospinal fluid (CSF) flow. The results showed that ciliary motility disorder could delay CSF exchange in the lateral ventricles.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Physiology
Tim Hsu-Han Wang, Timothy R. Angeli, Shunichi Ishida, Peng Du, Armen Gharibans, Niranchan Paskaranandavadivel, Yohsuke Imai, Taimei Miyagawa, Thomas L. Abell, Gianrico Farrugia, Leo K. Cheng, Gregory O'Grady
Summary: Loss of interstitial cells of Cajal (ICC) and aging are associated with higher gastric slow wave velocity, but the exact reasons for these relationships are still unknown. Increased slow wave velocity may modulate gastric emptying higher, although in gastroparesis other pathological factors must dominate to prevent emptying.
PHYSIOLOGICAL REPORTS
(2021)
Article
Physics, Fluids & Plasmas
Zhihan Huang, Toshihiro Omori, Takuji Ishikawa
Article
Physics, Fluids & Plasmas
Takeru Morita, Toshihiro Omori, Yohei Nakayama, Shoichi Toyabe, Takuji Ishikawa
Article
Cardiac & Cardiovascular Systems
Hiroki Kamada, Hideki Ota, Masanori Nakamura, Yohsuke Imai, Shunichi Ishida, Wenyu Sun, Ko Sakatsume, Ichiro Yoshioka, Yoshikatsu Saiki, Kei Takase
SEMINARS IN THORACIC AND CARDIOVASCULAR SURGERY
(2020)
Article
Physics, Fluids & Plasmas
Helene de Maleprade, Frederic Moisy, Takuji Ishikawa, Raymond E. Goldstein