Article
Chemistry, Analytical
Kyo-in Koo, Andreas Lenshof, Le Thi Huong, Thomas Laurell
Summary: This paper introduces a direct hydrogel extrusion process exposed to an ultrasound standing wave to align fibroblast cells into network structures, offering new insights for engineered organ and drug development. The ultrasound-exposed fibroblast cells displayed over 95% viability in culture medium for up to 10 days, showing promising potential for long-term applications.
Review
Biochemistry & Molecular Biology
Hidekazu Sekine, Teruo Okano
Summary: One of the key challenges in regenerative medicine is to introduce vascular networks into bioengineered tissues. Co-culturing transplant tissue with vascular cells before transplantation has been explored as a way to promote capillary angiogenesis and construct a continuous circulatory structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Lingjiao Zou, Weiqing Zhou, Xiangming Na, Naoki Asakawa, Hua Yue, Guanghui Ma
Summary: 3D cell carriers with porous structures have the potential to investigate drug treatments for tumors. The fabrication of gigantic highly-porous microcarriers allows for high-efficiency 3D cell culture and the construction of artificial tumor tissue models.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Review
Chemistry, Analytical
Honglin Shen, Shuxiang Cai, Chuanxiang Wu, Wenguang Yang, Haibo Yu, Lianqing Liu
Summary: Three-dimensional multicellular spheroids have great potential in biomedical research, providing a more accurate simulation of tissue structure and function. They have become important models for tumor research, drug screening, tissue engineering, and other fields.
Review
Nanoscience & Nanotechnology
Runxuan Cai, Naroa Gimenez-Camino, Ming Xiao, Shuguang Bi, Kyle A. A. DiVito
Summary: Tissue engineering is a promising technology for repairing and regenerating different types of biological tissues. Traditional approaches to skin tissue engineering have made progress but still have limitations. In vitro three-dimensional (3D) skin constructs are effective substitutes and have led to innovative discoveries. 3D bioprinting technology offers new opportunities for building functional human skin models.
REVIEWS ON ADVANCED MATERIALS SCIENCE
(2023)
Review
Engineering, Biomedical
Woochan Kim, Yonghyun Gwon, Sunho Park, Hyoseong Kim, Jangho Kim
Summary: Three-dimensional stem cell culture systems have the potential to mimic complex interactions between cells and the extracellular matrix, and have unique properties that can be applied in therapeutics.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Irene Chiesa, Carmelo De Maria, Giovanni Vozzi, Riccardo Gottardi
Summary: Each year, thousands of patients face life-threatening ear, nose, and throat disorders, such as tracheal stenosis, or conditions that affect their psychosocial well-being, such as microtia. Tissue engineering offers an exciting alternative by creating bioartificial constructs using three-dimensional scaffolds and human cells that can grow and develop. Bioprinting technologies, which utilize additive manufacturing, are particularly useful in addressing the complex geometries of ear, nose, and throat, allowing for the creation of patient-specific scaffolds with high design flexibility and repeatability.
Article
Chemistry, Multidisciplinary
Jianmei Chen, Xiaotong Wu, Yu Zhang, Yurui Xu, Haixiong Ge, Xinghai Ning
Summary: CellMatrix is a biomimetic 3D dynamic culture system with rational structure and function that improves cell supply. It can be directly implanted into defected tissues and improve tissue remodeling.
Review
Cell & Tissue Engineering
Young Hoon Son, Dae Hyeok Yang, Biaggio Uricoli, Sung-Jin Park, Gun-Jae Jeong, Heung Jae Chun
Summary: Tendon is a unique component of the musculoskeletal system that plays a crucial role in transmitting mechanical stress and enabling locomotor motion. Current treatment options for tendon injuries are limited and not very successful, leading to the emergence of biomedical engineering approaches. Three-dimensional cell culture platforms provide a more realistic environment and offer opportunities for new therapeutic strategies for tendon tissue regeneration.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Engineering, Biomedical
Qilong Zhao, Yu Zhou, Min Wang
Summary: This study demonstrates a new method for directly placing living endothelial cells within bioactive nanofibrous scaffolds in 3D, using concurrent emulsion electrospinning and coaxial cell electrospraying. The technique allows for deep cell distribution and preservation of cell viability, leading to promising bioactive nanofibrous scaffolds with 3D cell incorporation. By combining structural and biochemical cues, the 3D cell-incorporated scaffolds offer an innovative approach to tissue engineering for creating vascularized structures.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Multidisciplinary
Alperen Abaci, Gulden Camci-Unal, Murat Guvendiren
Summary: Three-dimensional (3D) bioprinting is an emerging technology that can fabricate functional tissues and organs, replicating native tissue function. With its precise positioning of cellular materials and utilization of medical images, 3D bioprinting has enormous potential in biomedical applications, such as tissue engineering and regenerative medicine. It is a rapidly progressing field that has shown clinically relevant uses.
Review
Polymer Science
M. Sai Bhargava Reddy, Deepalekshmi Ponnamma, Rajan Choudhary, Kishor Kumar Sadasivuni
Summary: Tissue engineering and regenerative medicine aim to restore or replace damaged tissues and organs using information and technology from various fields. Careful selection of scaffold materials and development of components are essential to achieve this goal.
Article
Engineering, Biomedical
Salwa Alshehri, Ram Karan, Sarah Ghalayini, Kowther Kahin, Zainab Khan, Dominik Renn, Sam Mathew, Magnus Rueping, Charlotte A. E. Hauser
Summary: Three-dimensional bioprinting is a promising method for tissue and organ engineering. However, the development of bioink materials and the diffusion barrier in scaffold materials limit its widespread use. In this study, genetically encoded gas vesicles derived from haloarchaea were used to promote oxygen diffusion in printed constructs. The results showed that cells printed with gas vesicles had increased activity and proliferation, suggesting that gas vesicles can overcome the diffusion barrier and increase oxygen availability in bioprinted constructs. The biodegradable nature of gas vesicle proteins makes them a potential oxygen-promoting material in biological samples.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Engineering, Biomedical
Ian C. Berg, Erfan Mohagheghian, Krista Habing, Ning Wang, Gregory H. Underhill
Summary: 3D microenvironments with different geometries have been used to investigate the impact on progenitor cell fate, revealing a positive correlation between intercellular tension and increased hepatocytic fate, as well as a negative correlation between compression and decreased hepatocytic fate with increased biliary fate.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Biomedical
Naiana Suter, Sophie Stebel, Carmela Rianna, Manfred Radmacher, Dorothea Bruegemann
Summary: Current knowledge on cell-biomaterial interactions is largely based on 2D cell culture systems, but transferring results to 3D protein scaffolds with biomimetic nanofiber structures remains a challenge. By fabricating binary collagen scaffolds with variable thickness and topographies, researchers were able to observe differences in cell responses to different protein topographies, indicating potential for further understanding topography-dependent cell recognition processes.
Article
Engineering, Biomedical
Lourdes Albina Nirupa Julius, Lukas Matter, Nils Schuergers, Johannes Luetzenkirchen, Vanessa Trouillet, Teba Gil-Diaz, Emil R. Mamleyev, Annegret Wilde, Vlad Badilita, Jan G. Korvink
Summary: This study investigated different substrates for studying phototactic twitching motility of cyanobacteria and found that surfaces with a water contact angle of 80 degrees showed increased cell motility compared to a reference glass surface. Plasma-treated PDMS surfaces exhibited similar motility to glass surfaces, while untreated PDMS surfaces showed close to zero motility.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Abhishek Sarkar, Di Wang, Mohana Kante, Luis Eiselt, Vanessa Trouillet, Gleb Iankevich, Zhibo Zhao, Subramshu S. Bhattacharya, Horst Hahn, Robert Kruk
Summary: Technologically relevant strongly correlated phenomena exhibited by perovskite manganites are enhanced by the coexistence of multiple competing magneto-electronic phases. The recently discovered high entropy oxides exhibit indications of an inherent magneto-electronic phase separation encapsulated in a single crystallographic phase. Combining the high entropy concept with standard property control, the study demonstrates the potential for a synergetic development of strongly correlated oxides offered by the high entropy design approach.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Daniel J. Carvalho, Anna M. Kip, Mirian Romitti, Marta Nazzari, Andreas Tegel, Matthias Stich, Christian Krause, Florian Caiment, Sabine Costagliola, Lorenzo Moroni, Stefan Giselbrecht
Summary: The function of the thyroid gland in the human body can be severely affected by endocrine disrupting chemicals (EDCs). Current in vitro assays based on 2D thyroid cell cultures are not accurate in evaluating the safety of these compounds. The development of a thyroid organoid-on-a-chip (OoC) device using polymeric membranous carriers is described in this study, which shows a high degree of functionality and represents a valuable tool for testing potential EDCs.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Panagiota Kakni, Barry Jutten, Daniel Teixeira Oliveira Carvalho, John Penders, Roman Truckenmuller, Pamela Habibovic, Stefan Giselbrecht
Summary: The gut microbiome is crucial for proper gut function, but imbalances can lead to gastrointestinal conditions. Current models do not accurately depict in vivo situations, thus more advanced in vitro models are needed to study host-microbiome interactions. Here, we developed a novel hypoxia-tolerant apical-out small intestinal organoid model that mimics intestinal cell composition and structure. Co-cultures with anaerobic strains showed successful colonization and probiotic benefits. These organoids will help unravel unknown mechanisms and aid in the development of microbiome-related therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Chemistry, Physical
Joana M. Ribeiro, Frederico J. Rodrigues, Filipe C. Correia, Inga Pudza, Alexei Kuzmin, Aleksandr Kalinko, Edmund Welter, Nuno P. Barradas, Eduardo Alves, Alec P. LaGrow, Oleksandr Bondarchuk, Alexander Welle, Ahmad Telfah, Carlos J. Tavares
Summary: Transparent ZnO:Sb thin films were deposited using magnetron sputtering, and their structure and disorder were studied. It was found that Sb doping influenced the crystalline structure and promoted partial amorphization. A film with 12 at% of Sb exhibited the highest Seebeck coefficient and thermoelectric power factor.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Materials Science, Multidisciplinary
Ilona Wagner, Simon Spiegel, Julian Brueckel, Matthias Schwotzer, Alexander Welle, Martina H. H. Stenzel, Stefan Braese, Salma Begum, Manuel Tsotsalas
Summary: Surface engineering of MOF NPs using dynamic modification strategies enables the tailoring of PEG-grafted MOF NPs with improved colloidal stability and high dispersity, while maintaining their morphology and lattice crystallinity. The PEG-grafted MOF NPs show reduced adsorption of HSA protein compared to uncoated ones, indicating the potential for reducing nonspecific interactions and introducing targeting moieties for specific interactions. This approach expands the applications of colloidal MOF NPs in biologically relevant fields.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Zeinab Mohamed Hassan, Wei Guo, Alexander Welle, Robert Oestreich, Christoph Janiak, Engelbert Redel
Summary: Gas-phase infiltration of the carbonylchloridogold(I), Au(CO)Cl precursor into the pores of HKUST-1 SURMOFs leads to the formation of ordered gold nanoclusters within the MOF matrix. These nanoclusters are homogeneous and distributed in the medium-sized pores of HKUST-1. The characterization of Au-x@HKUST-1 was performed using various techniques including SEM, IRRA, Raman spectroscopy, ToF-SIMS, XPS, and ICP-OES.
Article
Engineering, Biomedical
Danielle Baptista, Zeinab Tahmasebi Birgani, Helene Widowski, Fiona Passanha, Vasili Stylianidis, Kevin Knoops, Eva Gubbins, Cinta Iriondo, Kari-Pekka Skarp, Robbert J. Rottier, Tim G. Wolfs, Clemens van Blitterswijk, Vanessa LaPointe, Pamela Habibovic, Niki L. Reynaert, Stefan Giselbrecht, Roman Truckenmueller
Summary: This study investigated the feasibility of culturing human bronchial organoids in a polymer film-based microwell array platform. The organoids were preformed through the culture of single cells and then transferred into the microwells for further cultivation. Various microscopy techniques were employed to characterize the organoids, and the ease of micromanipulation in the microwells was demonstrated by microinjection.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Multidisciplinary
Salma Begum, Ksenia Kutonova, Anna Mauri, Meike Koenig, Ka Chun Chan, Christian Sprau, Christian Dolle, Vanessa Trouillet, Zahid Hassan, Tobias Leonhard, Stefan Heissler, Yolita M. Eggeler, Wolfgang Wenzel, Mariana Kozlowska, Stefan Braese
Summary: This study reports a distinct synthetic approach to develop disulfide-bridged 2D polymeric C3N3S3 triazine thin-films by interfacial thiol-disulfide dynamic exchange process. The resulting polymeric thin-films are covalently cross-linked, dynamic in nature, and feature tunable thickness and significant morphological variations. The thin films exhibit high reflectance transmittance, high refractive indices, and optical anisotropy.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Alexander Welle, Mohit Mehta, Karin Marek, Harry Peters, Peter Van der Wel, Olukayode Imole
Summary: This study investigated the impact of high shear blending conditions on the distribution of magnesium stearate on lactose particles. The results showed that magnesium stearate significantly affected the rheological properties of the blends, and blending speed had a strong influence on the distribution of magnesium stearate on lactose surface, while blending time had a minor effect.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Materials Science, Multidisciplinary
R. M. Jentner, S. P. Tsai, A. Welle, S. Scholl, K. Srivastava, J. P. Best, C. Kirchlechner, G. Dehm
Summary: Differentiation between granular bainite and polygonal ferrite in HSLA steels is challenging due to their similar mechanical and chemical properties. In this study, the kernel average misorientation from EBSD was used to classify and quantify the microstructural constituents. ECCI validation was performed to investigate the effect of grain tolerance angle on classification. The results showed that the automated EBSD classification approach based on local misorientation achieved reliable results with a grain tolerance angle of 5°.
JOURNAL OF MATERIALS RESEARCH
(2023)
Article
Endocrinology & Metabolism
Marta Nazzari, Mirian Romitti, Duncan Hauser, Daniel J. Carvalho, Stefan Giselbrecht, Lorenzo Moroni, Sabine Costagliola, Florian Caiment
Summary: This study demonstrates that despite a limited number of differentially expressed genes, various analysis methods can be used to obtain relevant information on phthalates, highlighting the potential of in vitro thyroid-related systems for the analysis of endocrine disruptors.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Gozde Sahin, Erik Vrij, Rhiannon Grant, Stefan Giselbrecht
Summary: Electrospinning is a powerful method for replicating the fibrous structure of the extracellular matrix. However, the random fiber deposition limits the reproduction of fiber mesh morphology on multiple samples. A multi-replication process combining electrospinning and imprinting is developed to accurately reproduce fiber morphology on different cell culture substrates. This process allows for controlled engineering of well-characterized cell microenvironments to investigate cell-material interactions.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Energy & Fuels
Penghui Zhu, Vanessa Trouillet, Stefan Heissler, Wilhelm Pfleging
Summary: Aqueous processing of cathode is a practical method to reduce cost and achieve environmentally friendly production for lithium-ion batteries. However, it can lead to lithium leaching and chemical corrosion, resulting in cavities inside dried electrodes. Acid additions and laser patterning were used to adjust the slurry pH value and generate three-dimensional structures in the thick-film electrodes.
JOURNAL OF ENERGY STORAGE
(2023)
Article
Engineering, Biomedical
Pinak Samal, Jay Rabindra Kumar Samal, Hoon Suk Rho, Denis van Beurden, Clemens van Blitterswijk, Roman Truckenmuller, Stefan Giselbrecht
Summary: This paper presents a novel method for fabricating subcellular structures in the commonly available material PMMA, and discusses the effects of different developers. It also highlights the fabrication of 3D curvilinear surfaces with microstructures and the production of multilayered microfluidic chips. The method can be used for selectively exposing stem cells to soluble factors by replicating microstructures.
MATERIALS TODAY BIO
(2023)