期刊
BIOMEDICAL MICRODEVICES
卷 10, 期 5, 页码 653-660出版社
SPRINGER
DOI: 10.1007/s10544-008-9176-5
关键词
microelectromechanical systems; two-axis microscanner; confocal microscopy; handheld probe; biomedical diagnostics; subcellular imaging
资金
- Wallace H. Coulter Foundation Early Career Award
- National Science Foundation grant [ECS 9731293, ECS 0335765]
We introduce a handheld single-fiber laser-scanning confocal microscope, incorporating a high-reflectivity two-axis silicon vertical combdrive microscanner, aimed at in vivo early detection of epithelial precancers. The approach adopted is motivated by need for a portable, economical, biopsy-free, early precancer screening technology in low-infrastructure environments. Our microelectromechanical system (MEMS) based handheld probe integrates the microscanners with miniature objective lens system and flexible electrical routing in a forward-imaging configuration, with 4.8 mm distal probe tip outer diameter for unrestricted imaging access in biological sites such as the oral cavity and cervix. Reflectance confocal videos of a USAF 1951 resolution target and biological samples were obtained over 200 mu m x 110 mu m field of view, with 0.80 and 9.55 mu m lateral and axial resolution, at 3.5-5.0 frames per second. With improvements to objective numerical aperture, our probe can enable precise evaluation of nuclear size, density, nucleus-to-cytoplasm ratio and cell density, which are important visual identifiers of epithelial precancers.
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