Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Materials Science, Biomaterials
Donagh G. O'Shea, Caroline M. Curtin, Fergal J. O'Brien
Summary: Articular cartilage in the human body plays a vital role in facilitating frictionless movement of synovial joints. However, its avascular and aneural nature limits its ability to self-repair when damaged. Current surgical treatment options lead to the formation of non-durable tissue, necessitating the need for a new solution. Recent advances in tissue engineering have aimed to recreate the microenvironment of native articular cartilage using biomaterial scaffolds, but the complexity of native tissue has proven to be a challenge. The advent of 3D printing has provided a potential solution by allowing the fabrication of biomimetic scaffolds that mimic the architecture and composition of articular cartilage.
BIOMATERIALS SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Natalia Linkova, Vladimir Khavinson, Anastasiia Diatlova, Svetlana Myakisheva, Galina Ryzhak
Summary: Finding innovative ways to treat osteoarthritis (OA) is a pressing task in molecular medicine and biogerontology. Directed differentiation of mesenchymal stem cells (MSCs) into chondrocytes is considered a potential method for OA treatment. This review examines the main molecules involved in the chondrogenic differentiation of MSCs, with peptides derived from growth factors' structures and components of cartilage tissue showing promising results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Zahra Soraya, Marzieh Ghollasi, Raheleh Halabian, Elahe Eftekhari, Amin Tabasi, Ali Salimi
Summary: The study showed that differentiated hMSCs on PLLA-DH exhibited higher ALP enzyme activity and biomineralization, more intense von Kossa staining, and increased expression of osteogenic gene and protein markers. This suggests that the combination of PLLA scaffold with DH has the potential to develop a suitable matrix for bone tissue engineering applications.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Xingyu Deng, Xiabin Chen, Fang Geng, Xin Tang, Zhenzhen Li, Jie Zhang, Yikai Wang, Fangqian Wang, Na Zheng, Peng Wang, Xiaohua Yu, Shurong Hou, Wei Zhang
Summary: The study utilized 3D printing technology with cryo-printing to create polyurethane scaffolds that mimic native meniscus, improving bioactivity for hMSCs culture. Surface modification with fibronectin was found to enhance stem cell functions and favor long-term chondrogenesis. The scaffolds successfully facilitated hMSCs chondrogenic differentiation and demonstrated good in vivo biocompatibility, highlighting their potential for meniscus regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ilona Uzieliene, Daiva Bironaite, Paulius Bernotas, Arkadij Sobolev, Eiva Bernotiene
Summary: Osteoarthritis is a chronic joint disease that leads to bone and cartilage deterioration, causing symptoms like joint stiffness and pain. Research is focusing on using various methods, including stem cells and chondrocytes, to improve cartilage tissue repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Valentina Rafaela Herrera Millar, Barbara Canciani, Laura Mangiavini, Joel Fernando Soares Filipe, Lucia Aidos, Margherita Pallaoro, Giuseppe Maria Peretti, Paola Pocar, Silvia Clotilde Modina, Alessia Di Giancamillo
Summary: This study evaluated the effect of COL18A1 on swine meniscal cells and found that a higher concentration of COL18A1 promotes a fibro-chondrogenic phenotype and improves cellularity index and cell efficiency. These findings lay the foundation for future studies evaluating the use of 3D endostatin hydrogel scaffolds in the differentiation of avascular tissues.
Article
Engineering, Biomedical
Monize Caiado Decarli, Adrian Seijas-Gamardo, Francis L. C. Morgan, Paul Wieringa, Matthew B. Baker, Jorge Vicente L. Silva, Angela Maria Moraes, Lorenzo Moroni, Carlos Mota
Summary: Cartilage tissue has limited self-repair ability and irreversible progression of lesions. Tissue engineering structures, such as extrusion bioprinted constructs containing stem cell spheroids, show promise for research and therapy. In this study, a reproducible bioprinting process followed by chondrogenic differentiation using hMSC spheroids in a xanthan gum-alginate hydrogel was successfully performed. The bioprinted constructs showed stable structure, cell viability, and matrix production, demonstrating their potential as stable chondral tissue implants.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Ye Sun, Jie Zhao, Qiang Wu, Yuxin Zhang, Yongqing You, Wenbo Jiang, Kerong Dai
Summary: This study investigates the feasibility of using engineered extracellular vesicles (EVs) with chondrogenic priming as a biomimetic tool for chondrogenesis. It demonstrates that EVs derived from TGF beta 3-preconditioned BMSCs contain specific miRNAs that can promote chondrogenesis in native BMSCs. Additionally, it shows that EVs rich in miR-455 from TGF beta 3-preconditioned BMSCs can alleviate osteoarthritis and promote cartilage regeneration through the SOX11/FOXO signaling pathway. The T3-EV hydrogel designed in this study also shows potential in cartilage defect treatment.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Engineering, Biomedical
Yan Xie, Linawati Sutrisno, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: Interconnected scaffolds promote the chondrogenic differentiation of stem cells. In this study, collagen scaffolds with interconnected pore structures were fabricated using PLGA sponge templates and were used to culture human bone marrow-derived mesenchymal stem cells (hMSCs). The results showed that the interconnected scaffolds can enhance the chondrogenic differentiation of stem cells and improve the production of cartilaginous matrices and the mechanical properties of the implants after subcutaneous implantation.
BIOMEDICAL MATERIALS
(2022)
Article
Engineering, Biomedical
Ahmad S. Arabiyat, Michaela R. Pfau, Melissa A. Grunlan, Mariah S. Hahn
Summary: Incorporating poly-L-lactic acid into poly(epsilon-caprolactone)-diacrylate shape memory polymers forms a semi-interpenetrating network with increased intrinsic osteoinductivity and accelerated degradation. Additionally, a bioinspired polydopamine coating further enhances the osteoinductive capacity of these scaffolds, leading to elevated expression of osteogenic markers and decreased chondrogenic and adipogenic responses.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Cell & Tissue Engineering
Daniele Zuncheddu, Elena Della Bella, Dalila Petta, Cecilia Bartschi, Sonja Hackel, Moritz C. Deml, Martin J. Stoddart, Sibylle Grad, Valentina Basoli
Summary: This study investigates the effects of glucose and fructose on the chondrogenic differentiation of BMSCs. The results show that fructose may enhance chondrogenic differentiation, while decreasing glucose concentration may decrease differentiation yield. The study also reveals the expression and regulation of GLUT3 and GLUT5 in BMSCs during chondrogenesis. The findings provide valuable insights into the sugar metabolism of BMSCs during cartilage formation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Caroline Melou, Pascal Pellen-Mussi, Solen Novello, Damien Brezulier, Agnes Novella, Sylvie Tricot, Pascale Bellaud, Dominique Chauvel-Lebret
Summary: The objective of this study was to develop a three-dimensional culture model for rapidly evaluating cartilage tissue engineering protocols. The spheroid model showed greater fluctuations in chondrogenesis markers compared to the gold standard pellet culture. Additionally, different cell lines from the same organ exhibited distinct biological responses.
Article
Biochemistry & Molecular Biology
Ching-Yun Chen, Chunching Li, Cherng-Jyh Ke, Jui-Sheng Sun, Feng-Huei Lin
Summary: The research aims to optimize the chondrogenic process in MSC-based constructs using Kartogenin (KGN) for potential use in cartilage tissue engineering. The results demonstrate that KGN treatment can enhance MSC condensation and promote active deposition of type II collagen and aggrecan, showing promise for better strategies in cartilage tissue engineering.
Article
Chemistry, Multidisciplinary
Yuna Moon, Madhumita Patel, Soyoun Um, Hyun Jung Lee, Sohee Park, Soo-Bong Park, Sun-Shin Cha, Byeongmoon Jeong
Summary: Dietary intake of folic acid promotes cartilage regeneration by enhancing the chondrogenic differentiation of tonsil-derived mesenchymal stem cells (TMSCs). The optimal concentration of folic acid treatment is 30 μM, where it significantly increases the expression of chondrogenic biomarkers without affecting osteogenic markers. However, high concentrations of folic acid may lead to a decrease in chondrogenic biomarkers, possibly due to DNA methylation.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Engineering, Biomedical
Haitao Cui, Wei Zhu, Yimin Huang, Chengyu Liu, Zu-Xi Yu, Margaret Nowicki, Shida Miao, Yilong Cheng, Xuan Zhou, Se-Jun Lee, Yifu Zhou, Suna Wang, Muhammad Mohiuddin, Keith Horvath, Lijie Grace Zhang
Article
Nanoscience & Nanotechnology
Se-Jun Lee, Darya Asheghali, Brianna Blevins, Raju Timsina, Timothy Esworthy, Xuan Zhou, Haitao Cui, Sung Yun Hann, Xiangyun Qiu, Alexander Tokarev, Sergiy Minko, Lijie Grace Zhang
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Nanoscience & Nanotechnology
Xuan Zhou, Margaret Nowicki, Hao Sun, Sung Yun Hann, Haitao Cui, Timothy Esworthy, James D. Lee, Michael Plesniak, Lijie Grace Zhang
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Nanoscience & Nanotechnology
Yue Wang, Haitao Cui, Yancheng Wang, Chengyao Xu, Timothy J. Esworthy, Sung Yun Hann, Manfred Boehm, Yin-Lin Shen, Deqing Mei, Lijie Grace Zhang
Summary: 4D printing enables the creation of pre-designed structures with time-dependent, dynamic shape changes to promote uniform cellular growth and distribution. The development of near-infrared light-sensitive cardiac constructs allows on-demand shape changes to mimic the curved topology of myocardial tissue.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Biomedical
Sung Yun Hann, Haitao Cui, Timothy Esworthy, Xuan Zhou, Se-jun Lee, Michael W. Plesniak, Lijie Grace Zhang
Summary: The development of vascular networks is crucial for tissue fabrication in regenerative medicine. 3D printing vascularized bone has gained attention due to bone disorders, but challenges persist due to the need for nanoscale precision. A novel 3D printing approach was combined with biocompatible materials to enhance vascular growth and tissue bioactivity.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Manufacturing
Sung Yun Hann, Haitao Cui, Margaret Nowicki, Lijie Grace Zhang
ADDITIVE MANUFACTURING
(2020)
Article
Materials Science, Biomaterials
Alejandro Herreros-Pomares, Xuan Zhou, Silvia Calabuig-Farinas, Se-Jun Lee, Susana Torres, Timothy Esworthy, Sung Yun Hann, Eloisa Jantus-Lewintre, Carlos Camps, Lijie Grace Zhang
Summary: Traditional two-dimensional (2D) in vitro cell cultures and animal models are limited in simulating the native environment for cancer stem cell (CSC) studies, but novel three-dimensional (3D) cell cultures show promise in enriching CSCs from lung cancer patients. Different 3D printing platforms were used to produce rigid and hydrogel-based scaffolds, with gene expression analysis confirming overexpression of stemness and invasion promoters in cells grown on hydrogel scaffolds and tumor spheres. The 3D printed models, especially those using GelMA-PEGDA hydrogels, outperform 2D culture models in mimicking tumor complexity and regulating cancer cell behavior.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Review
Materials Science, Biomaterials
Tarun Agarwal, Dishary Banerjee, Rocktotpal Konwarh, Timothy Esworthy, Jyoti Kumari, Valentina Onesto, Prativa Das, Bae Hoon Lee, Frank A. D. T. G. Wagener, Pooyan Makvandi, Virgilio Mattoli, Sudip Kumar Ghosh, Tapas Kumar Maiti, Lijie Grace Zhang, Ibrahim T. Ozbolat
Summary: This review focuses on the significance of using 3D bioprinting technology in liver tissue engineering, as well as the opportunities for stem cells and differentiation strategies for different types of liver cells.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Review
Materials Science, Biomaterials
Tarun Agarwal, Gabriele Maria Fortunato, Sung Yun Hann, Bugra Ayan, Kiran Yellappa Vajanthri, Dario Presutti, Haitao Cui, Alex H. P. Chan, Marco Costantini, Valentina Onesto, Concetta Di Natale, Ngan F. Huang, Pooyan Makvandi, Majid Shabani, Tapas Kumar Maiti, Lijie Grace Zhang, Carmelo De Maria
Summary: Bioprinting technologies have made significant advancements in the fabrication of engineered cardiac tissue, offering possibilities for functional cardiac tissue models for regenerative and diagnostic applications, while also discussing the related challenges and prospects.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Nathan J. Castro, Greta Babakhanova, Jerry Hu, K. A. Athanasiou
Summary: Traditional destructive tests are limited in their applicability to biomanufacturing, making nondestructive testing methods, such as photo- and acoustic-based techniques, more important. These methods allow for quantitative measurements of structural, compositional, and functional variances in soft tissues.
TRENDS IN BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Yue Wang, Haitao Cui, Timothy Esworthy, Deqing Mei, Yancheng Wang, Lijie Grace Zhang
Summary: The rapid development of 3D printing has had a significant impact on the field of biomedical engineering, particularly 4D printing. While there has been progress made in the development of 4D printing for tissue scaffolds and medical devices, there are limitations in printable smart materials and achievable responses with current stimulation sources. This review highlights recent progress in 4D printing, focusing on printing mechanisms, smart ink mechanisms, structural design, and printing technologies. It also summarizes recent studies on tissue/organ regeneration and medical device applications, and discusses current challenges and future perspectives of 4D printing.
ADVANCED MATERIALS
(2022)
Article
Acoustics
Jenna Osborn, Megan S. Anderson, Morgan Beddingfield, Lijie Grace Zhang, Kausik Sarkar
Summary: Scientists are facing challenges in creating effective biomimetic constructs in tissue engineering with sustained and controlled delivery of growth factors. They have explored acoustic droplet vaporization (ADV) in GelMA hydrogels and discovered the performance of different perfluorocarbon droplets. The results provide a potential application of ADV in hydrogel scaffolds for tissue engineering.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Sung Yun Hann, Haitao Cui, Timothy Esworthy, Lijie Grace Zhang
Summary: In this study, a two-in-one 4D smart cardiac construct that integrates the functions of minimally invasive cell vehicles and in situ tissue patches was developed for repairing damaged myocardial tissue.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Review
Materials Science, Biomaterials
Tarun Agarwal, Sung Yun Hann, Irene Chiesa, Haitao Cui, Nehar Celikkin, Simone Micalizzi, Andrea Barbetta, Marco Costantini, Timothy Esworthy, Lijie Grace Zhang, Carmelo De Maria, Tapas Kumar Maiti
Summary: The article discusses the combination of 3D printing technology and smart materials in additive manufacturing to create biomimetic structures that can dynamically reshape in response to external stimuli. This approach shows promise in various biomedical areas.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Surgery
Jonathan Minto, Xuan Zhou, Jenna Osborn, Lijie Grace Zhang, Kausik Sarkar, Raj D. Rao