Article
Chemistry, Multidisciplinary
Xucong Huang, Zhenghua Wu, Yanchao Liu, Mengqi Jia, Wenjuan Zhao, Shuowen Wang, Xinhui Jiang, Yuefen Lou, Guorong Fan
Summary: A simple, sensitive, and rapid LC-MS/MS method was developed for the simultaneous determination of BIC, M7, and M8 active metabolites in SD rat plasma. The method showed excellent linearity and accuracy for quantitation in plasma samples. Pharmacokinetic analysis revealed improved parameters in the BIC-NPs group compared to the BIC group, with a significant increase in AUC(0-t) for BIC and a double peak phenomenon observed for M7.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Lili Jin, Haibo Ding, Volkan Degirmenci, Hongchuan Xin, Qifan Miao, Qiuyu Wang, Dianbao Zhang
Summary: AWRK6, a synthesized peptide based on the natural peptide dybowskin-2CDYa, was analyzed in rat plasma using a LC-MS/MS method. Pharmacokinetic studies showed AWRK6 peaked in rat plasma at 1.2 hours after intraperitoneal injection and had a terminal half-life of approximately 2.8 hours.
Article
Biochemistry & Molecular Biology
Yang Li, Na Zhao, Tingting Zhang, Xinchi Feng
Summary: Physalin A, a natural product with promising anti-inflammatory and anti-tumor activities, was analyzed in rat plasma using a stable LC-MS/MS method. The developed method demonstrated good linearity and was successfully applied to pharmacokinetic studies of Physalin A in rats, providing valuable insights for future research on this compound.
Article
Biochemical Research Methods
Shengbo Huang, Yuanjin Zhang, Yanfang Zhang, Jie Liu, Zongjun Liu, Xin Wang
Summary: This study established an efficient and sensitive method for quantifying aloperine in rat plasma, and conducted pharmacokinetic studies in Sprague-Dawley rats. The method was validated in compliance with FDA bioanalytical method validation guidelines.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Daya Raju Adye, Sachin B. Jorvekar, Upadhyayula Suryanarayana Murty, Subham Banerjee, Roshan M. Borkar
Summary: Analytical sample preparation techniques are crucial for evaluating chemicals in different biological matrices, and the development of extraction techniques is a contemporary trend in bioanalytical sciences.
Article
Chemistry, Medicinal
Dong-Jin Hwang, Yali He, Suriyan Ponnusamy, Thirumagal Thiyagarajan, Michael L. Mohler, Ramesh Narayanan, Duane D. Miller
Summary: A major challenge in new drug discovery of androgen receptor antagonists is predicting the druggable properties necessary for potency and stability. This study synthesized new compounds with improved stability while maintaining potent antagonistic activity. Through a structure-activity relationship study, two compounds were found to be potent AR antagonists with improved in vivo pharmacokinetics and antiandrogen activity.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Dong-Jin Hwang, Yali He, Suriyan Ponnusamy, Thirumagal Thiyagarajan, Michael L. Mohler, Ramesh Narayanan, Duane D. Miller
Summary: A major challenge in new drug discovery is predicting the druggable properties of small molecules for potent and stable activity. In this study, new small molecules with improved stability and potent antagonistic activity against androgen receptor (AR) were synthesized. Structure-activity relationship (SAR) analysis identified two compounds with high efficacy as AR antagonists, exhibiting improved pharmacokinetics in vivo and antiandrogen activity in tumor xenograft models.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Xun Fang, Qi Lu, Jichun Han
Summary: AZD7648 is a potent DNA-PK inhibitor being developed for ovarian cancer treatment. This study developed a simple and sensitive LC-MS/MS method for determining the concentration of AZD7648 in rat plasma. The method was validated and proven to be selective, sensitive, and reliable, and successfully applied to pharmacokinetic study.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Biochemical Research Methods
Ting Yang, Wenwu Xu, Xiyu Wei, Zhenzhen Zhang, Yue Sun, Houru Liu, Peihua Yu, Wei Li, Dehong Yu
Summary: A simple, efficient, and sensitive method was developed and validated for the determination of GRh3 in rat plasma using LC-tandem mass spectrometry. The method demonstrated high accuracy, stability, and has been successfully applied to study the pharmacokinetics of GRh3 in rats.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Biochemical Research Methods
Nazli Erdogar, Tuba Recber, Alper B Iskit, Erem Bilensoy, Sedef Kir, Emirhan Nemutlu
Summary: The study developed a LC-MS/MS method for quantitative analysis of aprepitant in rat plasma, and evaluated the method's selectivity, linearity, accuracy, precision, robustness, and ruggedness according to ICH guidelines, showing that the method is accurate and reliable for the analysis of aprepitant in rat plasma samples.
Article
Biochemical Research Methods
Dan Chen, Kaixin Du, Xiaochen Niu, Hongwei Zhang, Xue Zhang, Yu Tang, Jianchun Zhao
Summary: A sensitive LC-MS/MS method for the determination of amlexanox in rat plasma was successfully established and validated, and applied to pharmacokinetic study. The method involved the use of Capcell Pak C18 column and triple quadrupole mass in MRM for detection, with calibration curves ranging from 50 to 2000 ng/mL.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Biochemistry & Molecular Biology
Xin Shen, Gaofu Li, Libin Wang, Huijin Yu, Lei Zhou, Huifang Deng, Ningning Wang, Chengcai Lai, Wei Zhou, Yue Gao
Summary: This study evaluated the pharmacokinetics of acipimox in rats under simulated high altitude hypoxia conditions. A sensitive LC-MS/MS method was established for quantification of acipimox in rat plasma and tissue homogenate. The results showed significant differences in the pharmacokinetics parameters of acipimox between normoxic and hypoxic rats. Changes in lipid metabolism-related proteins and free fatty acid levels were also observed.
Article
Chemistry, Analytical
Ruirui Chang, Si-Tao Xu, Hao-Wen Jin, Yong-Jie Zhao, Tian-Yu Hu, Guangji Wang, Jiankun Wang, Le Zhen
Summary: A LC-MS/MS method was developed for simultaneous determination of ZL-01 and its metabolites in rat plasma, considering stability issues. The method showed good linearity, precision, and accuracy, and was successfully applied in pharmacokinetic studies. Results suggested rapid metabolism of ZL-01 and identified M8 as a main metabolite after oral absorption.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Shailesh D. Dadge, Neerja Tiwari, Athar Husain, Saurabh Verma, Arun Agarwal, Richa Garg, Srikanta K. Rath, Karuna Shanker, Jiaur R. Gayen
Summary: This study aimed to develop and validate a sensitive and selective LC-ESI-MS/MS method for the biomarkers of WS NMITLI-118R AF1 and investigate their pharmacokinetics in rats. The biomarkers were found to be stable in both in-vivo and in-vitro conditions.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Zhen-miao Qin, Yong-hui Li, Yin-feng Tan, Hai-long Li
Summary: This study developed a rapid, sensitive, and specific LC-tandem mass spectrometry method for the determination of nootkatone in rat plasma. The method was successfully applied to pharmacokinetic study, showing low bioavailability of nootkatone. The method demonstrated acceptable precision and accuracy values in quantification.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Pharmacology & Pharmacy
Kyeong-Ryoon Lee, Jong-Woo Jeong, Hun-Chan Hyun, Eunseo Jang, Sunjoo Ahn, Sungwook Choi, Sang Hoon Joo, Sungsub Kim, Tae-Sung Koo
Article
Chemistry, Medicinal
Anand Balupuri, Myeong Hwi Lee, Sangeun Chae, Eunmi Jung, Woosub Yoon, Yunki Kim, So Jung Son, Jeonghee Ryu, Dae-Hyuck Kang, Young-Jae Yang, Ji-Na You, Hyunjin Kwon, Jong-Woo Jeong, Tae-Sung Koo, Dae-Yon Lee, Nam Sook Kang
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Pharmacology & Pharmacy
Jong-Hwa Lee, Hak Hyung Kim, Young Ho Cho, Tae-Sung Koo, Gye Won Lee
Article
Veterinary Sciences
Hyuck-Joo Yang, Ye-In Oh, Jong-Woo Jeong, Kun-Ho Song, Tae-Sung Koo, Kyoung-Won Seo
BMC VETERINARY RESEARCH
(2018)
Article
Chemistry, Multidisciplinary
Dae Hun Kim, Pooja Maharjan, Jae Yeol Kim, Dong-Jin Jang, Tae-Sung Koo, Kyoung Ah Min, Kwan Hyung Cho
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
(2019)
Article
Veterinary Sciences
Do-Hyun Seo, Jong-Bok Lee, Ji-Hye Hwang, Jong-Woo Jeong, Gun-Ho Song, Tae-Sung Koo, Kyoung-Won Seo
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2019)
Article
Ophthalmology
Ki-Hong Jang, Yun-Ju Do, Tae-Sung Koo, Jun-Sub Choi, Eun Ju Song, Yeseong Hwang, Hyun Ju Bae, Ju-hee Lee, Eunhee Kim
EXPERIMENTAL EYE RESEARCH
(2019)
Article
Chemistry, Analytical
Jong-Woo Jeong, Ji-Hoon Oh, Yu-Geun Ji, Yu-Mi Shin, Myeong Hwi Lee, Nam Sook Kang, Weontae Lee, Sung-Sub Kim, Tae-Yoon Kim, Tae-Sung Koo
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2019)
Article
Pharmacology & Pharmacy
Yoo-Kyung Song, Yun-Hwan Seol, Min Ju Kim, Jong-Woo Jeong, Hae-In Choi, Seung-Won Lee, Yoon-Jee Chae, Sunjoo Ahn, Young-Dae Gong, Kyeong-Ryoon Lee, Tae-Sung Koo
Summary: Supinoxin, a novel anticancer drug candidate, exhibited good permeability and dose-independent pharmacokinetics in rats. After oral administration, it showed modest absorption and high absolute oral bioavailability, mainly eliminated via NADPH-dependent phase I metabolism.
Article
Pharmacology & Pharmacy
Jong-Hwa Lee, Hyeong Sik Jeong, Jong-Woo Jeong, Tae-Sung Koo, Do-Kyun Kim, Young Ho Cho, Gye Won Lee
Summary: This study prepared an amorphous solid dispersion of rivaroxaban using hot-melt extrusion technique and optimized the formulation for improved solubility and bioavailability. The optimized RXB-ASD showed satisfactory results in in vitro drug dissolution and pharmacokinetic studies.
Article
Biochemistry & Molecular Biology
Ah Reum Han, Eun Hee Jeon, Kun Woo Kim, Seul Ki Lee, Chan-yeong Ohn, Sung Jean Park, Nam Sook Kang, Tae-Sung Koo, Ki Bum Hong, Sungwook Choi
Summary: In this article, we describe the design and synthesis of quinolin-2 (1H)-one derivatives that exhibit higher potency in inhibiting TTR amyloidogenesis compared to tafamidis. These compounds also show potential as fluorescent tools for TTR quantification, imaging sensors, and studying the mechanism of TTR amyloidogenesis.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Thi-Thao-Linh Nguyen, Jin Woo Kim, Hae-In Choi, Han-Joo Maeng, Tae-Sung Koo
Summary: In this study, a LC-MS/MS method was developed and validated for the quantitation of ARV-110 in rat and mouse plasma. The method showed good linearity and accuracy, and the elimination of ARV-110 was found to be unlikely in rat, mouse, and human hepatic microsomes. The oral bioavailability of ARV-110 was moderate in rats and mice.
Article
Chemistry, Medicinal
Eun-Sil Lee, Nayeon Kim, Joon Hee Kang, Aizhan Abdildinova, Seon-Hyeong Lee, Myung Hwi Lee, Nam Sook Kang, Tae-Sung Koo, Soo-Youl Kim, Young-Dae Gong
Summary: A new compound, DGG200064, was developed and shown to inhibit the growth of colorectal cancer cells by inducing G2/M arrest. It selectively inhibits the interaction of FBXW7 and c-Jun proteins, resulting in high cell growth inhibition efficacy. This compound has potential as a novel oral anti-colorectal cancer drug.
Article
Pharmacology & Pharmacy
Dong-Jin Jang, Jun Hak Lee, Da Hun Kim, Jin-Woo Kim, Tae-Sung Koo, Kwan Hyung Cho
Summary: The purpose of this study was to develop clear aqueous rebamipide (REB) eye drops with improved solubility, stability, patient compliance, and bioavailability. The pH-modification method using NaOH and a hydrophilic polymer was employed to prepare a super-saturated 1.5% REB solution. The optimized eye drops formulation (F18 and F19) demonstrated long-term physicochemical stability and hypotonicity, and showed significantly long-lasting pharmacokinetic results in a rat study.
Article
Chemistry, Analytical
Gi Ju Lee, Jin Woo Kim, Hae-In Choi, Jin Young Choi, Kwan Hyung Cho, Tae-Sung Koo
Summary: AZD5305, a selective PARP1 inhibitor, shows potential for treating ovarian and breast cancers caused by BRCA mutations. A LC-MS/MS method was developed and validated to quantify AZD5305 in mice plasma, and its pharmacokinetics were analyzed. The results demonstrate that AZD5305 has high stability and favorable pharmacokinetic properties.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2023)