期刊
BIOMEDICAL CHROMATOGRAPHY
卷 27, 期 11, 页码 1540-1546出版社
WILEY-BLACKWELL
DOI: 10.1002/bmc.2957
关键词
LC-MS; MS; indapamide; human whole blood; pharmacokinetics
A fast and simple liquid chromatography-electrospray ionization tandem mass spectrometry method for determination of indapamide in human whole blood was developed and validated. The sample extraction of indapamide from human whole blood was achieved using automated solid-phase extraction. Chromatographic separation was performed on Kinetex C-18 column (100x2.1mm, 1.7 mu m particle size) using acetonitrile and 2mm ammonium formate in ratio 90:10 (v/v) as a mobile phase. The mass spectrometer was operated in the multiple reaction monitoring mode using positive electrospray ionization for indapamide and the internal standard (zolpidem tartarate). The total run time was 2.5min. The present method was found to be linear in the concentration range of 1-50ng/mL with the coefficient of determination 0.9987. The absolute recoveries of indapamide were 90.51-93.90%. The method was validated according the recommendations for validation of bioanalytical methods of European Medicines Agency guideline and was successfully used to analyze human whole blood samples for application in a pharmacokinetic study. Copyright (c) 2013 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据