Article
Cell Biology
Chandranayaka Siddaiah, Anil Kumar BM, Saligrama Adavigowda Deepak, Syed Salman Lateef, Saurabh Nagpal, Kanchugarakoppal S. Rangappa, Chakrabhavi D. Mohan, Shobith Rangappa, Madan S. Kumar, Minaxi Sharma, Vijai Kumar Gupta
Summary: This study focused on metabolite profiling and identification from the bark of Alangium salviifolium by using organic and aqueous solvents. Eighty-one secondary metabolites with therapeutic potential were identified, showcasing the therapeutic value of this plant.
Article
Biochemistry & Molecular Biology
Yixing Lu, Wanying Zhang, Yongxiang Zhang, Sujuan Wu, Minglang Ma, Xianfeng Peng, Zhenling Zeng, Dongping Zeng
Summary: In this study, potential metabolic pathways and metabolites of isopropoxy benzene guanidine (IBG) were identified using high-performance liquid chromatography tandem mass spectrometry (UHPLC-Q-TOF-MS/MS). Seven metabolites were detected in rat liver microsomes, with hydroxylation being the main metabolic pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Jie Tang, Mengge Shi, Yan Xu, Zhengcai Ju, Huida Guan, Jun Lin, Gan Li, Han Han, Tong Zhang
Summary: A rapid and highly sensitive LC-MS/MS method was established and validated for simultaneous determination of multiple components in rat plasma, providing useful information for the further study of active ingredients of SGZT.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Laximan Velip, Vivek Dhiman, Bhoopendra Singh Kushwah, Vijaya Madhyanapu Golla, S. Gananadhamu
Summary: Urapidil, an antihypertensive drug, was investigated under various stress conditions according to ICH guidelines. A stability indicating HPLC method and LC-Q-TOF-MS were used to separate, identify, and evaluate the toxicity of five impurities. The study findings are important for understanding the stability of the drug and the mechanism of impurity formation.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jangmi Choi, Min-Ho Park, Seok-Ho Shin, Jin-Ju Byeon, Byeong Ill Lee, Yuri Park, Young G. Shin
Summary: The study found that the concentration of SAS in mouse brain was much lower than in plasma, with only a small percentage of SAS metabolized into significant metabolites SP and Ac-SP. The results suggest that metabolites such as SP and Ac-SP may be responsible for the pharmacological effects in the brain, rather than SAS itself.
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Food Science & Technology
Yingying Zheng, Long Tang, Mingguang Yu, Ting Li, Huanlu Song, Pei Li, Ku Li, Jian Xiong
Summary: Research should focus on developing novel agents to replace salt in foods without affecting taste, to avoid health risks associated with high-salt diets. Some yeast extracts can be used as salt substitutes without imparting additional odors. Salty peptides were fractionated from Angel Yeast in this study.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Plant Sciences
Zain Nurazah, Abu Seman Idris, Amiruddin Mohd Din, Mohamad Arif Abd Manaf, Abrizah Othman, Umi Salamah Ramli
Summary: This study compared the metabolic profiles of natural Ganoderma-infected and healthy control oil palm root samples, revealing differential metabolites associated with BSR in oil palm roots. Systematic metabolic pathway analysis identified the significant involvement of amino acid metabolism, carbohydrate metabolism, and biosynthesis of other secondary metabolites in response to BSR disease.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Review
Food Science & Technology
Minke Yang, Juan Li, Chengying Zhao, Hang Xiao, Xiang Fang, Jinkai Zheng
Summary: Flavonoids have gained increasing attention for their various health-promoting effects. Liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF-MS/MS) has become a widely used technique for detecting flavonoids, providing efficient separation and structural information. This review comprehensively summarizes the principles and methodologies of LC-Q-TOF-MS/MS for detecting flavonoids in food, including qualitative and quantitative determination. It also discusses recent applications in agricultural products, food processing, and in vivo metabolism, as well as suggesting technological improvements and potential applications.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Guo-Ming Dong, Hang Yu, Li-Bin Pan, Shu-Rong Ma, Hui Xu, Zheng-Wei Zhang, Pei Han, Jie Fu, Xin-Yu Yang, Adili Keranmu, Hai-Tao Niu, Jian-Dong Jiang, Yan Wang
Summary: The study revealed that gut microbiota plays a significant role in the metabolism of timosaponin BII, resulting in the production of various metabolites, including three newly identified structures in the gut microbiota. The metabolic behavior of timosaponin BII in gut microbiota system was found to be unique compared to rat liver homogenate and a rat liver microsome incubation system.
Article
Food Science & Technology
Nannan Li, Shuqin Li, Qirou Wang, Shuyu Yang, Yinuo Hou, Yan Gao, Xiangyang Zhang, Min Zhang, Haixia Chen
Summary: This study applied MALDI-TOF MSI and QTOF LC-MS/MS techniques to investigate the spatial distribution changes of low molecular weight compounds in garlic tissue during Laba garlic processing. It was found that some bioactive compounds were transformed or leached out during the processing, while new compounds related to pigments emerged. This study provides a basis for understanding the spatial distributions and changes of compounds in garlic tissue during Laba garlic processing, which may affect the bioactivities of garlic.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Daseul Jeong, Sanggil Choe, Sooyeun Lee, Kang Min Kim, Jaesung Pyo
Summary: The metabolites of five major illegal anti-obesity drugs were analyzed for the first time, providing a tool for toxicological detection and metabolite identification in forensic cases. The study revealed that these drugs were metabolized into multiple constituents in in vivo and in vitro models.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Ophthalmology
Ai Lin Daphne Teh, Jaime Jacqueline Jayapalan, Mun Fai Loke, Azida Juana Wan Abdul Kadir, Visvaraja Subrayan
Summary: This study used a non-targeted metabolomics approach to investigate metabolite differences between patients with keratoconus and control subjects, identifying potential serum biomarkers. The identified metabolites, including dehydroepiandrosterone sulfate and eicosanoids, showed significant upregulation in keratoconus patients. Glycerophospholipid PS(17:2(9Z,12Z)/20:4 (5Z,8Z,11Z,14Z)) was significantly upregulated in control subjects. These differentially regulated metabolites provide insights into the pathophysiology of keratoconus and could potentially be utilized as biomarkers for early diagnosis and monitoring of the disease.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Chenxiao Shan, Jia Li, Po Sun, Runze Zhou, Min Xu, Qiulong Zhao, Ping Ren, Hongmei Wen, Xi Huang
Summary: A sensitive, precise, and rapid method was developed in this study for the identification of absorbed compounds in the plasma of depressed rats and for the pharmacokinetic analysis. The study provides key information on the chemical and pharmacokinetic profiles of XYSJW, which is important for its therapeutic efficacy and further pharmacological studies.