期刊
BIOMEDICAL CHROMATOGRAPHY
卷 27, 期 4, 页码 509-514出版社
WILEY-BLACKWELL
DOI: 10.1002/bmc.2820
关键词
isoquercitrin; intestinal bacteria; UPLC-Q-TOF; MS; metabolites
类别
资金
- Jiangsu Province Colleges and Universities Natural Science Major Basic Research Projects [10KJA360039]
- Construction Project for Jiangsu Key Laboratory for High Technology Research of TCM Formulae [BM2010576]
- National Basic Research Program of China (973 Program) [2011CB505300, 2011CB505303]
In this paper, ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and the MetaboLynx software combined with mass defect filtering were applied to identity the metabolites of isoquercitrin using an intestinal mixture of bacteria and 96 isolated strains from human feces. The human incubated samples collected for 72 h in the anaerobic incubator and extracted with ethyl acetate were analyzed by UPLC-Q-TOF/MS within 10 min. The parent compound and five metabolites were identified by eight isolated strains, including Bacillus sp. 17, Veillonella sp. 23 and 32 and Bacteroides sp. 40, 41, 56, 75 and 88 in vitro. The results indicate that quercetin, acetylated isoquercitrin, dehydroxylated isoquercitrin, hydroxylated quercetin and hydroxymethylated quercetin are the major metabolites of isoquercitrin. Furthermore, a possible metabolic pathway for the biotransformation of isoquercitrin was established in intestinal flora. This study will be helpful for understanding the metabolic route of isoquercitrin and the role of different intestinal bacteria in the metabolism of natural compounds. Copyright (c) 2012 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据