期刊
BIOMEDICAL CHROMATOGRAPHY
卷 26, 期 1, 页码 1-5出版社
WILEY-BLACKWELL
DOI: 10.1002/bmc.1615
关键词
metformin; phenformin; biguanides; whole blood; LC-MS; MS
Metformin is an anti-diabetic drug in the biguanide class which also includes phenformin and buformin. Because of the potential adverse effects of the biguanides, a reliable liquid chromatographytandem mass spectrometry method using pneumatically assisted electrospray ionization was developed for the quantification of the drugs in both live and post-mortem human whole blood. The blood proteins were precipitated by the addition of a mixture of methanol and acetonitrile, and the extract was cleaned up by cation-exchange solid-phase extraction to eliminate ion suppression effects. The separation was performed by hydrophilic interaction liquid chromatography. Matrix-matched calibrants combined with isotope dilution of metformin were used for calibration. The detection limits were 0.01 mg/L for metformin and phenformin and the relative intra-laboratory reproducibility standard deviations were less than 6% at concentrations of 110 mg/L. The mean true recoveries were greater than 86%.
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