期刊
BIOMEDICAL CHROMATOGRAPHY
卷 24, 期 12, 页码 1350-1355出版社
WILEY
DOI: 10.1002/bmc.1448
关键词
hollow fiber liquid-phase microextraction; high-performance liquid chromatography; aristolochic acid; urine sample
类别
资金
- National Key Technology Research and Development Program of China [2007BAI37B05]
- Chinese Academy of Sciences
A simple and efficient hollow fiber liquid-phase microextraction (HF-LPME) technique in conjunction with high-performance liquid chromatography is presented for extraction and quantitative determination of aristolochic acid I in human urine samples. Several parameters influencing the efficiency of HF-LPME were investigated and optimized, including extraction solvent, stirring rate, extraction time, pH of donor phase and acceptor phase. Excellent sample clean-up was observed and good linearity with coefficient of 0.9999 was obtained in the range of 15.4-960 mu g/L. This method provided a 230-fold enrichment factor and good repeatability with relative standard deviations (RSD) lower than 6.0%. The limit of detection value for the analyte in urine samplewas 0.01 mu g/L at a signal-to-noise ratio of 3. The extraction recovery from urine samples was 61.8% with an RSD of 9.71%. Copyright (C) 2010 John Wiley & Sons, Ltd.
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