期刊
ANALYTICA CHIMICA ACTA
卷 881, 期 -, 页码 148-154出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.04.008
关键词
Electrochemiluminescence; Epidermal growth factor receptor; EGF-cytosensor
资金
- National Natural Science Foundation of China [21375048]
- Major State Basic Research Development Program of China (973 Program) [2010CB833603]
A sensitive electrochemiluminescence (ECL) strategy for evaluating the epidermal growth factor receptor (EGFR) expression level on cell surfaces was designed by integrating the specific recognition of EGFR expressed on MCF-7 cell surfaces with an epidermal growth factor (EGF)-funtionalized CdS quantum dots (CdSQDs)-capped magnetic bead (MB) probe. The high sensitivity of ECL probe of EGF-funtionalized CdSQD-capped-MB was used for competitive recognition with EGFR expressed on cell surfaces with recombinant EGFR protein. The changes of ECL intensity depended on both the cell number and the expression level of EGFR receptor on cell surfaces. A wide linear response to cells ranging from 80 to 4 x 10(6) cells mL(-1) with a detection limit of 40 cells mL(-1) was obtained. The EGF-cytosensor was used to evaluate EGFR expression levels on MCF-7 cells, and the average number of EGFR receptor on single MCF-7 cells was 1.35 x 10(5) with the relative standard deviation of 4.3%. This strategy was further used for in-situ and real-time evaluating EGFR receptor expressed on cell surfaces in response to drugs stimulation at different concentration and incubation time. The proposed method provided potential applications in the detection of receptors on cancer cells and anticancer drugs screening. (C) 2015 Elsevier B.V. All rights reserved.
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