Article
Chemistry, Analytical
Neda Azizipour, Rahi Avazpour, Michael H. Weber, Mohamad Sawan, Abdellah Ajji, Derek H. Rosenzweig
Summary: The use of a surface-optimized PDMS microfluidic biochip allows for the reproducible generation of uniform multicellular spheroids, which can more accurately predict cellular responses to drugs in vitro. This platform has important clinical implications for developing new therapeutic agents and optimizing existing treatment strategies in personalized medicine.
Review
Cell Biology
Marlene Geyer, Karla Queiroz
Summary: PDAC, the most common type of pancreatic cancer, is characterized by early metastasis and often requires chemotherapy treatment. With only 20% of tumors resectable, there is an urgent need for innovative modeling platforms to better understand and treat this complex disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medicine, Research & Experimental
Xin-yue Fan, Zhuo-fen Deng, Yan-yan Yan, Valerii E. Orel, Andrii Shypko, Valerii B. Orel, Donika Ivanova, Christian Pilarsky, Jing Tang, Zhe-Sheng Chen, Jian-ye Zhang
Summary: With the advancement of microfluidic chip technology, drug screening has become faster, more efficient, and higher throughput. This article summarizes the recent progress of microfluidic chip technology in drug screening, including the developmental history, structural design, and applications.
BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2022)
Review
Chemistry, Analytical
Yiwei Shi, Ying Cai, Yuhong Cao, Zhanying Hong, Yifeng Chai
Summary: To accelerate the discovery of anti-cancer drugs, there is an urgent need to establish an inexpensive and simple preclinical model that can simulate the tumor microenvironment and screen the drug candidates. Microfluidic chips are a promising technology due to low reagent consumption and the capability of replicating a more representative 3D cell culture.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Qiao Xiong, Ting Liu, Yidie Ying, Xiaowen Yu, Ziwei Wang, Hongliang Gao, Tianhai Lin, Weihua Fan, Zhensheng Zhang, Qiang Wei, Yuqing Ge, Shuxiong Zeng, Chuanliang Xu
Summary: This study established a bladder cancer spheroids microfluidic drug sensitivity analysis system, which accurately assesses the response of bladder cancer to different chemotherapeutic agents and correlates with the clinical response of patients.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Roman G. Szafran, Benita Wiatrak
Summary: In this study, the generation, stability, and linearity of molecular gradient in high-throughput drug screening microfluidic assay (HTS) were thoroughly analyzed. Mathematical models were developed to predict concentration profiles, and the precision of the equations was validated with low prediction error and high correlation coefficient. The newly established mathematical relationships became part of the new HTS biochip operating protocols, contributing to improved precision and accuracy in analytical procedures.
Article
Oncology
Albert Manzano-Munoz, Jose Yeste, Maria A. Ortega, Fernando Martin, Anna Lopez, Jordi Rosell, Sandra Castro, Cesar Serrano, Josep Samitier, Javier Ramon-Azcon, Joan Montero
Summary: Dynamic BH3 profiling (DBP), a functional assay, can predict treatment efficacy in cancer. To overcome the requirement of a large number of cells, researchers developed an innovative microfluidic-based DBP (mu DBP) device and validated its feasibility on gastrointestinal stromal tumor (GIST) patient samples.
NPJ PRECISION ONCOLOGY
(2022)
Article
Engineering, Biomedical
Qiangqiang Tang, Xiaoyu Li, Chen Lai, Lei Li, Hongkai Wu, Yingjun Wang, Xuetao Shi
Summary: In this study, a microfluidic chip using HA as a component was developed to create a highly bionic bone environment, which was found to be more conducive to cell proliferation and osteogenic differentiation compared to PDMS. The chip successfully generated a concentration gradient of model drug and determined the drug's IC50, showing great potential in high-throughput bone-related drug screening and research.
BIOACTIVE MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Seokgyu Han, Sein Kim, Hye Kyung Hong, Yong Beom Cho, Hyo Eun Moon, Sun Ha Paek, Sungsu Park
Summary: Tumor spheroids are useful for drug screening and understanding tumor physiology. The hanging drop method is suitable for high-throughput screening (HTS) of anticancer drugs, but needs to increase liquid-holding capacity. The multi-inlet spheroid generator (MSG) enables the addition of liquid-containing drugs or cells into a spheroid without increasing pressure. It is a versatile platform for HTS of anticancer drugs and replicating the tumor microenvironment (TME).
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Oncology
Milad Moloudizargari, Mohammad Hossein Asghari, Seyed Fazel Nabavi, Diana Gulei, Ioana Berindan-Neagoe, Anupam Bishayee, Seyed Mohammad Nabavi
Summary: In recent years, cancer research has made continuous advances in identifying new aspects of tumorigenesis and understanding the mechanisms behind cancer cell proliferation, invasion, drug resistance, and immortality. The Hippo signaling cascade, which determines cell fate, has been found to play a crucial role in this process. Dysregulation of this pathway has been observed in various tumors and contributes to their transformation, survival, growth, and resistance to therapy. As more is discovered about the Hippo pathway's interactions with other signaling pathways, researchers have focused on developing therapeutic interventions targeting its components. Natural plant-derived compounds have shown promise in this regard. This review summarizes the current understanding of the Hippo pathway and recent evidence on using natural compounds derived from plants to target its components.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Oncology
Jie Liu, Shengjun Sun, Cheng Zhou, Zhihong Sun, Qin Wang, Chengming Sun
Summary: NF-kappa B transcription factors play a critical role in important cellular processes. This study identified Lycorine as an inhibitor of the NF-kappa B signaling pathway, with potential anticancer activity against prostate cancer.
Article
Nanoscience & Nanotechnology
Yue Wu, Yuwen Zhao, Yuyuan Zhou, Khayrul Islam, Yaling Liu
Summary: Currently, the most commonly used in vitro tumor model for drug testing is the two-dimensional cell monolayer, but it fails to replicate the natural tumor microenvironment. This study presents an integrated engineering system to generate vessel-supported tumor models for preclinical drug screening. It provides a more realistic tumor microenvironment and has the potential to improve the efficiency of drug discovery.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yvonne Kohl, Margit Biehl, Sarah Spring, Michelle Hesler, Vladimir Ogourtsov, Miomir Todorovic, Joshua Owen, Elisabeth Elje, Kristina Kopecka, Oscar Hernando Moriones, Neus G. Bastus, Peter Simon, Tibor Dubaj, Elise Runden-Pran, Victor Puntes, Nicola William, Hagen von Briesen, Sylvia Wagner, Nikil Kapur, Espen Mariussen, Andrew Nelson, Alena Gabelova, Maria Dusinska, Thomas Velten, Thorsten Knoll
Summary: Microfluidic technology provides a valuable tool for creating in vitro models that capture cellular and organ level responses for rapid and animal-free risk assessment of new chemicals and drugs. These cell-based devices allow high-throughput screening and flexible automation, reducing costs and reagent consumption due to miniaturization. The interconnected modules aim to simulate whole-body exposure and response, contributing to the replacement of animal testing in risk assessment studies in compliance with the 3Rs principles.
Article
Engineering, Biomedical
Guocheng Fang, Hongxu Lu, Russul Al-Nakashli, Robert Chapman, Yingqi Zhang, Lining Arnold Ju, Gungun Lin, Martina H. Stenzel, Dayong Jin
Summary: This study presents a method to model peristalsis in human colon tumor organoids on a microfluidic chip. The chip allows the control of peristalsis amplitude and rhythm, and enables high throughput culture of organoids. Results show the importance of mimicking mechanical stimuli in the physiological environment when evaluating nanoparticles in vitro.
Review
Biochemistry & Molecular Biology
Irena Wieleba, Kamila Wojas-Krawczyk, Pawel Krawczyk, Janusz Milanowski
Summary: Despite significant progress in modern therapies, lung cancer remains a leading cause of death. Proper planning of preclinical research using 3D tumor microenvironment in vitro models is crucial for developing new anti-cancer drugs. The challenge lies in choosing the best tool for studying tumor microenvironments, especially in diverse lung cancer cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Xiaojian Shao, Dan Gao, Yongli Chen, Feng Jin, Guangnan Hu, Yuyang Jiang, Hongxia Liu
ANALYTICA CHIMICA ACTA
(2016)
Article
Biochemical Research Methods
Yongli Chen, Dan Gao, Hangrui Bai, Hongxia Liu, Shuo Lin, Yuyang Jiang
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2016)
Article
Chemistry, Analytical
Yongli Chen, Dan Gao, Yanwei Wang, Shuo Lin, Yuyang Jiang
ANALYTICA CHIMICA ACTA
(2018)
Article
Chemistry, Analytical
Yongli Chen, Deependra Tyagi, Mingsheng Lyu, Andrew J. Carrier, Collins Nganou, Brian Youden, Wei Wang, Shufen Cui, Mark Servos, Ken Oakes, Shengnan He, Xu Zhang
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Yongli Chen, Wei Wang, Deependra Tyagi, Andrew J. Carrier, Shufen Cui, Shengnan He, Xu Zhang
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)