Article
Medicine, General & Internal
Rudy Foddis, Maria Franzini, Alessandra Bonotti, Riccardo Marino, Roberto Silvestri, Poupak Fallahi, Dante Chiappino, Michele Emdin, Aldo Paolicchi, Alfonso Cristaudo
Summary: This study evaluated the role of specific GGT fractional enzymatic activity patterns in the diagnosis of MPM and found a specific profile of GGT fractions activity significantly associated with MPM. Combining GGT activity with serum SMRP levels improved the diagnostic accuracy.
Article
Agriculture, Multidisciplinary
Lichuang Cao, Qian Li, Rene Lametsch
Summary: In this study, GGT was purified from bovine milk and identified as a glycosylated membrane protein composed of two subunits. It can generate gamma-glutamyl peptides, with glutamine acting as a donor for the generation of these peptides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jian Gao, Wenjuan Chen, Weixuan Lin, Wei Yuan, Haihong Liu, Hongyang Xie, Qian Zhang, Peng Zhang, Caifeng Ding
Summary: A novel fluorescent probe NSA-GGT was designed and synthesized in this study for the detection of GGT activity, providing assistance for early diagnosis and treatment of tumors. This probe exhibited good sensitivity and performance for GGT, suitable for detecting GGT activity in living cells and animal tissues.
Article
Agriculture, Multidisciplinary
Tatsuo Fukao, Hideyuki Suzuki
Summary: In this study, gamma-glutamylvalylglycine was enzymatically synthesized with favorable taste properties, and its production was increased by adjusting reaction conditions and adding metal cations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Poh Kei Chong, Sue Lian Mun, Lee Sin Chang, Abdul Salam Babji, Seng Joe Lim
Summary: This study fractionated freeze-dried and spray-dried edible bird's nest hydrolysates using gel permeation chromatography and compared their chemical properties and antioxidant activities. The results showed that fractions with different molecular weights exhibited different properties and activities.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Medical Laboratory Technology
Xiaoan Ke, Linjie Wang, Lian Duan, Hongbo Yang, Jia Yin, Ling Qiu, Hui Pan, Fengying Gong, Huijuan Zhu
Summary: This study aimed to explore the feasibility of ultrafiltration (UF) and polyethylene glycol (PEG) precipitation for macroprolactin screening, as well as the incidence and clinical characteristics of Chinese patients with macroprolactinemia. The results showed a high correlation between UF and PEG precipitation, with PEG precipitation having smaller variation and substantial diagnostic consistency with gel filtration chromatography (GFC). The incidence of macroprolactinemia in Chinese patients with hyperprolactinemia (HPRL) was found to be low in this study. We recommend using PEG precipitation screening only for patients clinically suspected of having macroprolactinemia.
CLINICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Jorge L. Diaz-Gomeza, Laura -Margarita Lopez-Castillo, Silverio Garcia-Lara, Fabiola Castorena-Torres, Robert Winkler, Natalie Wielsch, Oscar Aguilar
Summary: In this study, the cytotoxic activity of alpha-zein hydrolysates obtained by enzymatic treatments on HepG2 cells was evaluated. Two fractions of the hydrolysates showed significant cytotoxic activity, and twelve possible peptide sequences with potential cell regulatory activities were predicted. This study highlights the importance of food-derived peptides in the development of anticancer pharmaceuticals.
FOOD AND BIOPRODUCTS PROCESSING
(2022)
Article
Biochemistry & Molecular Biology
Ying-Hui Li, Yu-Sheng Wang, Jing-Song Zhao, Zi-Yan Li, Hai-Hua Chen
Summary: By incorporating alginate-porous starch solution as the gel matrix and slow-gelling agents, a curcumin-loaded microemulsion gel with improved drug loading efficiency and capacity was successfully prepared. The denser structure that entrapped all microemulsion droplets was observed, and drug release followed a diffusion-controlled mechanism depending on the components and pH value.CUR-ME-G with a curcumin concentration of 0.20% exhibited the best biological activity, suggesting its potential application in smart drug delivery systems.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemical Research Methods
Wei Yang, Zhen Guo, Yanping Zhou, Jing Du, Huibin Liu, Jingnian Jia, Yuhuan Shen, Yongjian Chen
Summary: The optimized method for screening macroprolactinemia using 20% PEG6000 as a precipitant can completely precipitate macroPRL components and effectively retain freePRL components. Compared with traditional methods, the optimized method is simpler, more accurate, and more stable for the quantitative detection of freePRL.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Food Science & Technology
L. P. Pui, S. A. Mohammed, H. M. Ghazali
Summary: In this study, the enzyme 5'-PDE was successfully purified from adzuki beans using protein purification techniques, with measurements of purification yield, fold, and specific activity. The molecular weight of adzuki bean 5'-PDE was estimated through experiments and gel electrophoresis analysis.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2021)
Article
Food Science & Technology
Atia Gohar, Nida Dastagir, Almas Jabeen, M. Kamran Azim
Summary: The protein component of honey has therapeutic activities in inflammation and parasitic infections, with different types of honey showing varying anti-inflammatory potential. The study revealed that different natural honeys contain unique protein fractions with the ability to suppress ROS production, suggesting a tissue-protective role in alleviating oxidative stress.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2021)
Article
Gastroenterology & Hepatology
Shujie Pang, Yang Shi, Dapeng Xu, Zhe Sun, Yiming Chen, Yingcheng Yang, Xijun Zhao, Hui Si-ma, Ning Yang
Summary: This study established a diagnostic model to predict early recurrence of hepatocellular carcinoma based on the GAR value, with tumor size, number, BCLC stage, microvascular invasion, and GAR value identified as independent risk factors. The predictive model performed well, accurately predicting 1, 3, and 5-year disease-free survival and demonstrating better clinical usability compared to the BCLC staging system.
JOURNAL OF GASTROINTESTINAL SURGERY
(2022)
Article
Endocrinology & Metabolism
Camilla Bogelund Larsen, Eva Rabing Brix Petersen, Martin Overgaard, Steen Joop Bonnema
Summary: In a case where a young male presented with persistently elevated TSH levels but clinically and biochemically demonstrated euthyroidism, it was found that the patient harbored macro-TSH, a rare condition where the complex binding of TSH to other plasma proteins leads to elevated plasma TSH levels while the biologically active fraction remains normal.
EUROPEAN THYROID JOURNAL
(2021)
Article
Medical Laboratory Technology
Anna Friess, Ulrich Friess, Maria Shipkova, Eberhard Wieland
Summary: This study evaluates the feasibility of simultaneously measuring urinary gamma-glutamyltransferase (gamma GT) and lactate dehydrogenase (LDH) to distinguish fresh from previously frozen specimens in urine drug monitoring. The results demonstrate that this measurement method can reliably determine the enzyme activity of urinary gamma GT and LDH, and the measurement results can accurately predict the storage mode of urine samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Medicine, Research & Experimental
Somaya Albhaisi, Rehan Qayyum
Summary: Elevated levels of serum alanine and aspartate aminotransferase (ALT and AST) were associated with a U-shaped relationship with cancer mortality, while both the lowest and highest levels of these enzymes were linked to increased risk. Higher levels of alkaline phosphatase (ALP) were also associated with higher cancer mortality. Gamma-glutamyltransferase (GGT) levels did not show a consistent relationship with cancer mortality, but an increase after median was associated with higher mortality risk. Further exploration is needed to understand the mechanisms behind these relationships.
CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Luigi Emilio Pastormerlo, Silvia Burchielli, Marco Ciardetti, Giovanni Donato Aquaro, Chrysantos Grigoratos, Vincenzo Castiglione, Angela Pucci, Maria Franzini, Assuero Giorgetti, Paolo Marzullo, Eleonora Benelli, Silvia Masotti, Veronica Musetti, Fabio Bernini, Sergio Berti, Claudio Passino, Michele Emdin
Summary: This study investigated the potential role of sympathetic renal denervation (RD) in limiting myocardial damage and remodeling immediately after acute myocardial infarction (AMI) in pigs. The results showed that RD performed after AMI led to smaller necrotic areas, improved myocardial salvage index, higher cardiac output, and lower LV end diastolic volume compared to the control group. RD also resulted in reduced sympathetic activation and better cardiac function, indicating its potential for improving outcomes following AMI.
CLINICAL RESEARCH IN CARDIOLOGY
(2021)
Article
Biochemical Research Methods
Vanessa Susini, Laura Caponi, Veronica Lucia Rossi, Antonio Sanesi, Nadia Romiti, Aldo Paolicchi, Maria Franzini
Summary: The study found that the free sulfhydryl groups in the hinge region of monovalent antibody fragments (rIgG) play a crucial role in the orientation of antibodies on immunosensors. Both chemical reduction and orientation affect the signal enhancement, with rIgG generally showing greater signal enhancement capacity.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Beatrice Casini, Benedetta Tuvo, Emanuele Marciano, Giuliana Del Magro, Giulia Gemignani, Grazia Luchini, Maria Luisa Cristina, Anna Laura Costa, Guglielmo Arzilli, Michele Totaro, Angelo Baggiani, Gaetano Privitera
Summary: The study highlighted significant issues with non-compliance in the reprocessing of endoscopes, particularly in relation to high-concern organisms. However, improvements were seen through implementing corrective actions and regular microbiological surveillance. The key focus was on sampling the internal channels of duodenoscopes.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Environmental Sciences
Filippo Graziani, Rossana Izzetti, Lisa Lardani, Michele Totaro, Angelo Baggiani
Summary: The study on the dynamics of aerosol particles during dental procedures found that submicron aerosol particles slightly increased after instrumentation but slowly decreased afterwards, while larger particles did not show significant increases.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Review
Infectious Diseases
Guglielmo Arzilli, Giuditta Scardina, Virginia Casigliani, Davide Petri, Andrea Porretta, Marco Moi, Ersilia Lucenteforte, Jordi Rello, Pierluigi Lopalco, Angelo Baggiani, Gaetano Pierpaolo Privitera, Lara Tavoschi
Summary: This study investigated screening practices on admission to hospital wards and the risk of developing infection from colonisation. It found that the burden of antimicrobial-resistant Gram-negative bacteria (AMR-GNB) colonisation and the risk of progression to infection vary significantly across regions, pathogens, and time in hospitals.
JOURNAL OF INFECTION
(2022)
Article
Medicine, General & Internal
Rudy Foddis, Maria Franzini, Alessandra Bonotti, Riccardo Marino, Roberto Silvestri, Poupak Fallahi, Dante Chiappino, Michele Emdin, Aldo Paolicchi, Alfonso Cristaudo
Summary: This study evaluated the role of specific GGT fractional enzymatic activity patterns in the diagnosis of MPM and found a specific profile of GGT fractions activity significantly associated with MPM. Combining GGT activity with serum SMRP levels improved the diagnostic accuracy.
Review
Cardiac & Cardiovascular Systems
Alberto Aimo, Vincenzo Castiglione, Claudio Rapezzi, Maria Franzini, Giorgia Panichella, Giuseppe Vergaro, Julian Gillmore, Marianna Fontana, Claudio Passino, Michele Emdin
Summary: This review discusses the strategies for TTR silencing in the treatment of ATTR amyloidosis, including small interfering RNAs, antisense oligonucleotides, and gene editing approaches.
NATURE REVIEWS CARDIOLOGY
(2022)
Article
Virology
Arianna Baronti, Francesco Gentile, Alice Chiara Manetti, Andrea Scatena, Silvia Pellegrini, Angela Pucci, Maria Franzini, Vincenzo Castiglione, Aniello Maiese, Alberto Giannoni, Mauro Pistello, Michele Emdin, Giovanni Donato Aquaro, Marco Di Paolo
Summary: This study presents five cases of sudden death secondary to myocardial infarction (MI) closely related to COVID-19 vaccination. Pathological analysis and genetic testing revealed that the patients carried pro-thrombotic mutations, suggesting a possible link between COVID-19 vaccination, pro-thrombotic genotypes, and acute cardiovascular events that requires further investigation.
Article
Genetics & Heredity
Francesco Pasqualetti, Celeste Giampietro, Nicola Montemurro, Noemi Giannini, Giovanni Gadducci, Paola Orlandi, Eleonora Natali, Paolo Chiarugi, Alessandra Gonnelli, Martina Cantarella, Cristian Scatena, Giuseppe Nicolo Fanelli, Antonio Giuseppe Naccarato, Paolo Perrini, Gaetano Liberti, Riccardo Morganti, Maria Franzini, Aldo Paolicchi, Giovanni Pellegrini, Guido Bocci, Fabiola Paiar
Summary: This study found that systemic immunity and inflammation indexes (SI) may serve as potential biomarkers for survival in patients with glioblastoma. The results of univariate and multivariate regression analyses showed that certain indexes had a statistically significant impact on overall survival.
Review
Biochemistry & Molecular Biology
Chiara Sanguinetti, Marianna Minniti, Vanessa Susini, Laura Caponi, Giorgia Panichella, Vincenzo Castiglione, Alberto Aimo, Michele Emdin, Giuseppe Vergaro, Maria Franzini
Summary: This article provides an overview of the biological life cycle of transthyretin (TTR), including its physiological metabolism and the role of mutations and physiological ligands in its stability. Understanding these mechanisms is crucial for a better comprehension of the pathogenesis of TTR amyloidosis.
Article
Immunology
Laura Caponi, Alice Botti, Nadia Romiti, Aldo Paolicchi, Maria Franzini
Summary: Antibody light chains are synthesized in excess by plasma cells and can be secreted into biological fluids in different forms. Structural differences between monomers and dimers may affect their biological functions and pathogenicity. A simple preparatory procedure allows for the purification of FLC monomers and dimers from urine samples of patients with plasma cell disorders.
IMMUNOLOGIC RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Paolo Morfino, Alberto Aimo, Giuseppe Vergaro, Chiara Sanguinetti, Vincenzo Castiglione, Maria Franzini, Marco Alfonso Perrone, Michele Emdin
Summary: Transthyretin (TTR) amyloid cardiomyopathy (ATTR-CM) is a cause of heart failure characterized by misfolded TTR monomers deposited as amyloid fibrils in the myocardium. Standard treatment with TTR-stabilizing ligands may not be effective in advanced disease. Amyloid seeding, a self-propagating process, may further accelerate amyloid aggregation. Inhibition of amyloidogenesis combined with anti-seeding peptides and alternative strategies, such as TTR silencers and immunological amyloid disruptors, show promising results.
Article
Biochemistry & Molecular Biology
Chiara Sanguinetti, Valentina Scalise, Tommaso Neri, Alessandro Celi, Vanessa Susini, Maria Franzini, Roberto Pedrinelli
Summary: Gamma-glutamyl transferase (GGT) plays a role in the progression of atherosclerosis by promoting the generation of reactive oxygen species. It can also induce tissue factor expression, independently of its enzymatic activity. In this study, it was found that GGT-induced tissue factor stimulation is mediated by binding to Toll-like receptor 4, which is expressed on monocytes and colocalizes with GGT activity in atherosclerotic plaques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Letter
Public, Environmental & Occupational Health
Angelo Baggiani, Silvia Briani, Grazia Luchini, Mauro Giraldi, Jacopo Fernandez, Carla Collecchi, Matteo Filippi, Lucia Trillini, Domenica Mamone, Maria Carola Martino, Antonella Ciucci, Michele Cristofano, Antonella De Vito, Gabriella Pellegrini, Grazia Valori, Guglielmo Arzilli, Daniele Sironi, Tommaso Mariotti, Francesca Papini, Virginia Casigliani, Giuditta Scardina, Giacomo Visi, Beatrice Casini, Andrea Porretta, Michele Totaro, Gaetano Privitera, Carlo Milli
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2021)
