Article
Chemistry, Multidisciplinary
Yuto Honda, Sayaka Onodera, Hiroyasu Takemoto, Noor Faizah Che Harun, Takahiro Nomoto, Makoto Matsui, Keishiro Tomoda, Yudi Sun, Yutaka Miura, Nobuhiro Nishiyama
Summary: This study aims to develop a technology to control the activity of small interfering RNA (siRNA) in a thermo-responsive manner, even at temperatures close to body temperature. By conjugating siRNA with a thermally responsive copolymer, the activity of siRNA can be enhanced based on temperature changes. This technique offers a promising approach to selectively induce therapeutic effects of siRNA in vivo.
PHARMACEUTICAL RESEARCH
(2023)
Article
Engineering, Biomedical
Jin Qiu, Qianqian Fan, Sainan Xu, Dongmei Wang, Juntong Chen, Sainan Wang, Tianhui Hu, Xinran Ma, Yiyun Cheng, Lingyan Xu
Summary: This study discovered a highly efficient fluoropolypeptide for the delivery of siRNA-based therapeutics into adipocytes and adipose tissues, showing potential for gene therapy of obesity and metabolic disorders.
Article
Biochemistry & Molecular Biology
Simone Berger, Ana Krhac Levacic, Elisa Hoerterer, Ulrich Wilk, Teoman Benli-Hoppe, Yanfang Wang, Ozgur Ozturk, Jie Luo, Ernst Wagner
Summary: The study optimized lipophilic oligoaminoamides (OAAs) based on succinoyl tetraethylene pentamine (Stp) for pDNA delivery, finding that OAAs with fatty acid chain lengths around C6 to C10 exhibited higher gene transfer efficiency. Promising compounds showed moderate in vivo efficiency, indicating the need for further refinement.
Article
Chemistry, Multidisciplinary
Esther H. Roh, Thomas H. Epps, Millicent O. Sullivan
Summary: The article introduces a mathematical framework that can predict siRNA-mediated gene silencing with minimal experimental data and guide nanocarrier design. It reveals that siRNA dilution is a key determinant of gene silencing kinetics, and the experimental and predicted results align closely in vitro and in vivo settings.
Article
Biochemistry & Molecular Biology
Mohammad Shafee Alkanawati, Marina Machtakova, Katharina Landfester, Heloise Therien-Aubin
Summary: The development of responsive nanogel systems for drug delivery is challenging but promising due to their high stability in aqueous media and ability to respond to biochemical cues. In this study, multistimuli-responsive nanogels were synthesized using bio-orthogonal and reversible reactions, allowing for selective release of encapsulated cargos. These nanogels were pH-responsive and could be controlled for reversible release of payloads, and additionally responsive to enzymatic cleavage and oxidoreductive cues.
Article
Chemistry, Multidisciplinary
Mahadi Hasan, Tatsuya Fukuta, Shinya Inoue, Hinako Mori, Mayuko Kagawa, Kentaro Kogure
Summary: This study demonstrates the utility of iontophoresis as a local drug delivery technology for nucleic acid therapeutics. It enables direct delivery of these therapeutics to internal organs without the use of drug delivery carriers via non-blood circulatory pathways.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Zhao Wang, Jingjing Sun, Mingrui Li, Ting Luo, Yulin Shen, Amin Cao, Ruilong Sheng
Summary: By utilizing natural steroids, a series of positively-charged nanoparticles were synthesized, with their transfection efficiency depending greatly on the steroid hydrophobic tail structures and block ratios. The optimized nanoplexes showed transfection efficiency reaching around one-third of the traditional complexes.
Article
Engineering, Biomedical
Martijn J. W. Evers, Simonides van de Wakker, Ellis M. de Groot, Olivier G. de Jong, Jerney J. J. Gitz-Francois, Cor S. Seinen, Joost P. G. Sluijter, Raymond M. Schiffelers, Pieter Vader
Summary: EV-liposome hybrid nanoparticles have been investigated as a potential siRNA delivery system, demonstrating their ability to encapsulate siRNA, contain EV-surface markers, and functionally deliver siRNA to different cell types. These hybrids combine the benefits of both synthetic and biological drug delivery systems, showing altered functional behavior compared to liposomes and varying efficacy among recipient cell types.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Anna M. Rydzik, Dirk Gottschling, Eric Simon, Wioletta Skronska-Wasek, Joerg F. Rippmann, Doris Riether
Summary: The incorporation of N6-methyladenosine (m6 A) into siRNA targeting Factor VII affects its potency and selectivity in cells, reducing off-target effects. The position of m6 A in the siRNA duplex is crucial, as some sequences are found to be more potent and/or selective than their non-methylated counterparts. These findings suggest a potential regulatory role of N6-methyladenosine in the RNAi pathways and expand the scope of available chemical modifications for siRNA therapeutics.
Article
Pharmacology & Pharmacy
Dalila Miele, Xin Xia, Laura Catenacci, Milena Sorrenti, Silvia Rossi, Giuseppina Sandri, Franca Ferrari, John J. Rossi, Maria Cristina Bonferoni
Summary: Oligonucleotide therapeutics such as miRNAs and siRNAs are developed to modulate gene expression, but face issues of instability and easy degradation. Viral and non-viral vectors are used for efficient delivery, with non-viral vectors like polymeric nanocarriers providing safer and more effective means of delivery.
Review
Materials Science, Biomaterials
Jian Sun, Edikan Archibong Ogunnaike, Xing Jiang, Zhaowei Chen
Summary: Nanotechnology-based combination anticancer therapy shows promise in overcoming the limitations of single-agent administration by combining siRNA technology with chemotherapy. The challenges in fabricating siRNA/chemotherapeutic drug co-loaded nanovectors include adequate cargo protection, precise targeted delivery, and site-specific cargo release. This review summarizes recent developments in nanosystems for co-delivering siRNA and chemotherapeutic drugs and discusses their combined therapeutic effects.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Nanoscience & Nanotechnology
Danyang Li, Momina Ahmed, Anisah Khan, Lizhou Xu, Adam A. Walters, Belen Ballesteros, Khuloud T. Al-Jamal
Summary: The study demonstrates the controlled grafting of cationic polymers on carbon nanotubes, revealing the highly architectural-dependent behavior of different brush-CNT hybrids in binding with nucleic acids. Furthermore, the different chemistries of the brush coatings were found to influence biocompatibility, cellular uptake, and silencing efficiency in vitro.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Materials Science, Biomaterials
Xiaoyan Song, Zujian Feng, Yuanyuan Peng, Siyuan Yu, Xinjing Du, Pingsheng Huang, Weiwei Wang, Jinfeng Xing
Summary: In this study, paclitaxel (PTX) and curcumin (Cur) were encapsulated into nanogels (NG-PC) for the synergistic suppression of breast tumors. NG-PC showed controlled and cumulative release of PTX and Cur, as well as a remarkable killing effect against cancer cells. In vivo evaluation on tumor-bearing mice demonstrated that NG-PC had significantly higher antitumor ability compared to a single drug. This research provides a simple method to prepare a nano-drug delivery platform with excellent drug co-loading ability and synergistic antitumor effect.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Atsushi Uno, Kenji Arima, Masako Shimazaki, Maki Ushida, Kanako Amano, Reiko Namikawa, Kazuo Sakurai
Summary: This study introduces a novel technology that delivers oligonucleotide therapeutics to immune cells via SPG-Dectin-1 interaction. The results demonstrate the effectiveness of this approach in mice and humans, showing potential for treating autoimmune diseases, allergies, and cancer.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Yang Gao, Xingyu Chen, Taoran Tian, Tao Zhang, Shaojingya Gao, Xiaolin Zhang, Yangxue Yao, Yunfeng Lin, Xiaoxiao Cai
Summary: Researchers have developed a dynamic lysosome-activated tetrahedral framework nucleic acid (tFNA) nanobox for encapsulating siRNA and achieving controlled release in cells, demonstrating excellent RNA protection and release effects.
ADVANCED MATERIALS
(2022)
