期刊
BIOMACROMOLECULES
卷 12, 期 2, 页码 441-449出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm1012212
关键词
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资金
- National Institutes of Health [1-R15-EB008858-01]
- U.S. Civilian Research and Development Foundation [UKB2-2923-KV-07]
- National Science Foundation [CHE-0832622, CHE-0840431]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0840431] Funding Source: National Science Foundation
Targeted molecular imaging with two-photon fluorescence microscopy (2PFM) is a powerful technique for chemical biology and, potentially, for noninvasive diagnosis and treatment of a number of diseases. The synthesis, photophysical studies, and bioimaging are reported for a versatile norbornene-based block copolymer multifunctional scaffold containing biocompatible (PEG), two-photon fluorescent dyes (fluorenyl) and targeting (cyclic-RGD peptide) moieties. The two bioconjugates, containing two different fluorenyl dyes and cRGDfK covalently attached to the polymer probe, formed a spherical micelle and self-assembled structure in water, for which size was analyzed by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Cell viability and 2PFM imaging of human epithelial U87MG cell lines that overexpress alpha(v)beta(3) integrin was performed via incubation with the new probes, along with negative control studies using MCF-7 breast cancer cells and blocking experiments. 2PFM microscopy confirmed the high selectivity of the biocompatible probe in the integrin-rich area in the U87MF cells while blocking as well as negative control MCF-7 experiments confirmed the integrin-targeting ability of the new probes.
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