期刊
BIOMACROMOLECULES
卷 12, 期 6, 页码 2293-2301出版社
AMER CHEMICAL SOC
DOI: 10.1021/bm200369e
关键词
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资金
- NIH [AI080965]
- International Aids Vaccine Initiative [SFP-1851R]
Multivalent display of heterologous proteins on viral nanoparticles forms a basis Tor numerous applications in nanotechnology, inducing vaccine development, targeted therapeutic delivery, and tissue-specific bioimaging. In many instances, precise placement of proteins is required for optimal functioning of the supramolecular assemblies, but orientation- and site-specific coupling of proteins to viral scaffolds remains a significant technical challenge. We have developed two strategies that allow for controlled attachment of a variety of proteins on viral particles using covalent and noncovalent principles. In one strategy, an interaction between domain 4 of anthrax protective antigen and its receptor was used to display multiple copies of a target protein on virus-like particles. In the other, expressed protein ligation and aniline-catalyzed oximation was used to display covalently a model protein. The latter strategy, in particular, yielded nanoparticles that induced potent immune response's to the coupled protein, suggesting potential applications in vaccine development.
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