Article
Chemistry, Physical
Li Ye, Hongmei Liu, Xin Fei, Ding Ma, Xiaozhen He, Qianyun Tang, Xue Zhao, Hanbing Zou, Xiaojing Chen, Xianming Kong, Peifeng Liu
Summary: A surface modification strategy was used to enhance the endosomal escape ability of dendrimer DGL polymers, improving gene transfection efficiency. This strategy involved designing a library of PBA-modified DGL polymers to screen for efficient siRNA vectors, with one candidate showing nearly 90% gene silencing in cells. Furthermore, the PBA modification not only improved siRNA cellular uptake but also endowed DGL polymers with the ability to escape endosomes.
Article
Biochemistry & Molecular Biology
Dandan Zhao, Anh Thi Tram Tu, Miwako Shobo, Nguyen Bui Thao Le, Chiaki Yoshikawa, Kazuhisa Sugai, Yoji Hakamata, Tomohiko Yamazaki
Summary: In this study, the ε-PLL/G4-CpG ODN complex was successfully synthesized as a carrier, providing better stability against nucleases and significantly improved cellular uptake. Modulation of the N/P ratio successfully tuned the levels of immune cytokine production.
Article
Biotechnology & Applied Microbiology
Angel Espinoza-Gonzalez, Carmen Hernandez-Valencia, Luis Cedeno-Caero, Roberto Sanchez-Sanchez, Carmina Montiel, Miquel Gimeno
Summary: Subtilisin Carlsberg, an alkaline protease from Bacillus licheniformis, can catalyze the synthesis of high molecular weight cationic alpha-poly-L-lysine and amphiphilic poly(alpha-L-lysine-co-L-phenylalanine) in organic solvent. The reaction is conducted in hydrophobic non-toxic 1,1,1,2-tetrafluoroethane solvent, and the low water environment limits hydrolysis of the peptide chains. The organic compressed fluid used in the reaction has low-pressure requirements and evaporates completely after the reaction, leaving no solvent residues. The resulting polypeptides show no cytotoxicity in human cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Jie Cheng, Wenying Tu, Zhou Luo, Xinghua Gou, Qiang Li, Dan Wang, Jingwen Zhou
Summary: This study successfully established a synthetic pathway for biobased production of 5AVA from L-Lysine, resulting in a high yield of 52.24g/L through Fed-batch biotransformation. Computational analyses were used to identify potential mutation sites and propose strategies for trait improvement, offering new insights for efficient 5AVA synthesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Long Pan, Cunjin Zhang, Xinyu Yuan, Yu Zhang, Xusheng Chen, Cuizhu Tian, Zishan Zhang, Mengqing Tian, Aimei Liao, Guanghai Yu, Ming Hui, Xin Zeng, Jihong Huang
Summary: In this study, a co-culture fermentation strategy was used to enhance the production of epsilon-poly-L-lysine (epsilon-PL). A strain that could be co-cultured with Streptomyces albulus was screened and the conditions of co-culture fermentation were optimized. The production of epsilon-PL increased by 31.47% compared to single strain fermentation, and the amount of bacteria increased by 19.62%, indicating an improved synthesis ability. This study shows the potential of co-culture fermentation for large-scale epsilon-PL production and provides a new fermentation strategy.
FERMENTATION-BASEL
(2023)
Article
Engineering, Environmental
Junqing Lin, Wencheng Hu, Tao Gao, Bingbo Bao, Xingwei Li, Tengli Huang, Yi Sun, Junjie Shen, Hannan Xu, Kaiyuan Zhu, Hongyi Zhu, Yunlong Yang, Xianyou Zheng
Summary: The study evaluated the potential of epsilon-PLL as a cost-effective, biosafe, and effective CMC for drug delivery to cartilage. The results showed strong distribution and retention of epsilon-PLL in cartilage, with no negative impact on chondrocytes or local tissue, indicating its effectiveness and safety for DMOAD delivery.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
Sijin Chen, Shuting Huang, Yan Li, Chuncai Zhou
Summary: This review summarized the advanced development in the synthesis and modification strategies of ε-poly-L-lysine (EPL) and lysine-based dendrimer (LBD) compounds, focusing on their applications in biomedical fields such as agents, adjuvants, and carriers for anti-pathogen, tumor, and gene therapies. The combination with other advanced methodologies and technologies shows potential for overcoming challenges like scale production and high expenses in the future development of lysine-based polymers.
FRONTIERS IN CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Liang Wang, Yue Deng, Mengping Wu, Jianhua Zhang, Hongjian Zhang, Zhonggui Mao, Xusheng Chen
Summary: A dynamic pH-regulation strategy was developed to maintain and improve the production of e-poly-L-lysine (e-PL). The strategy effectively increased the production and productivity of e-PL by enhancing key enzyme activity, oxidative phosphorylation, ATP levels, ROS levels, and cellular antioxidant systems.
PROCESS BIOCHEMISTRY
(2022)
Article
Agricultural Engineering
Cuihuan Zhao, Taoran Zheng, Yinghao Feng, Xuan Wang, Lizhan Zhang, Qitiao Hu, Jinchun Chen, Fuqing Wu, Guo-Qiang Chen
Summary: This study successfully engineered Halomonas spp. for the production of L-lysine and cadaverine by manipulating gene expression and metabolic pathways.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hao Yang, Daojun Zhu, Lang Kai, Liang Wang, Hongjian Zhang, Jianhua Zhang, Xusheng Chen
Summary: In this study, a PPK-mediated ATP regeneration system was proposed and developed in S. albulus for the first time, significantly enhancing epsilon-PL production. The study provides an efficient approach to improve the production of not only epsilon-PL but also other ATP-driven metabolites.
MICROBIAL CELL FACTORIES
(2023)
Article
Chemistry, Multidisciplinary
Jie Yu, Danwei Cen, Yongcheng Chen, Hailan Zhao, Mengyue Xu, Sulan Wu, Shuo Wang, Qiao Jin, Ting Shen
Summary: Antibiotic resistance poses a significant threat to public health, and improving the therapeutic efficacy of conventional antibiotics is an effective solution. By conjugating the positively charged epsilon-poly-l-lysine to erythromycin, the modified EPL-EM showed enhanced antibacterial activity and reduced drug resistance development. Furthermore, EPL-EM exhibited excellent biocompatibility without inducing hemolysis and cytotoxicity.
Article
Biotechnology & Applied Microbiology
Gavin Gonzales, Jiaul Hoque, Anna Gilpin, Biswanath Maity, Stefan Zauscher, Shyni Varghese
Summary: This study focuses on the development of cationic nanocarriers to overcome the challenges of drug delivery to cartilage tissue. These nanocarriers, which adhere quickly to cartilage tissue and can penetrate and remain within its full thickness, show promising results for therapeutic outcomes.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Biotechnology & Applied Microbiology
Dahong Wang, Hemin Wang, Jinpeng Wu, Yuxin Hou, Jianrui Sun, Jiangfeng Yuan, Shaobin Gu
Summary: Epsilon-poly-L-lysine (ε-PL) is widely used in food and pharmaceutical industries due to its excellent antimicrobial activity and high safety. Recent studies have focused on biosynthetic mechanisms, strategies for improving production, and applications of ε-PL. Potential challenges and future prospects for ε-PL production were also proposed.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Chemistry, Applied
Hao Wu, Shuqian Hu, Chunling Nie, Jiahui Zhang, Huafeng Tian, Weicheng Hu, Ting Shen, Jianguo Wang
Summary: A novel method was described for synthesizing antibacterial cellulose beads, which exhibited excellent antimicrobial activity and biodegradability, showing great potential for use in food and environmental applications.
CARBOHYDRATE POLYMERS
(2021)
Review
Biotechnology & Applied Microbiology
Liang Wang, Chongyang Zhang, Jianhua Zhang, Zhiming Rao, Xueming Xu, Zhonggui Mao, Xusheng Chen
Summary: The review compares traditional and advanced strategies for discovering and cultivating high-producing strains of epsilon-poly-L-lysine, including traditional methods based on isolation and culture, genome mining, and directed evolution. Additionally, process engineering methods for improving production and efficient downstream purification methods are discussed in the article.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Etienne Robino, Aurore C. Poirier, Hajar Amraoui, Sandra Le Bissonnais, Angelique Perret, Carmen Lopez-Joven, Jean-Christophe Auguet, Tristan P. Rubio, Chantal Cazevieille, Jean-Luc Rolland, Yann Hechard, Delphine Destoumieux-Garzon, Guillaume M. Charriere
ENVIRONMENTAL MICROBIOLOGY
(2020)
Review
Chemistry, Multidisciplinary
Gregoire Danger, Louis Le Sergeant d'Hendecourt, Robert Pascal
NATURE REVIEWS CHEMISTRY
(2020)
Article
Pharmacology & Pharmacy
Imen Hadjadji, Mohamed Laabir, Hocine Frihi, Yves Collos, Zhao Jun Shao, Patrick Berrebi, Eric Abadie, Zouher Amzil, Nicolas Chomerat, Jean Luc Rolland, Fabien Rieuvilleneuve, Estelle Masseret
Article
Environmental Sciences
Marion Richard, Jean Luc Rolland, Yannick Gueguen, Julien de Lorgeril, Juliette Pouzadoux, Behzad Mostajir, Beatrice Bec, Sebastien Mas, David Parin, Patrik Le Gall, Serge Mortreux, Annie Fiandrino, Franck Lagarde, Gregory Messiaen, Martine Fortune, Emmanuelle Roque D'Orbcastel
Summary: The mortality of Crassostrea gigas juveniles is systematically observed every year worldwide, primarily due to Pacific Oyster Mortality Syndrome (POMS) caused by OsHV-1 infection. During the oyster mortality episode, releases of OsHV-1 and opportunistic bacteria were observed in the water, potentially impacting disease spread within oyster farms and marine biodiversity in shellfish farming areas.
MARINE ENVIRONMENTAL RESEARCH
(2021)
Article
Food Science & Technology
Damien Reveillon, Veronique Savar, Estelle Schaefer, Julien Cheve, Marie-Pierre Halm-Lemeille, Dominique Hervio-Heath, Marie-Agnes Travers, Eric Abadie, Jean-Luc Rolland, Philipp Hess
Summary: Tetrodotoxins have been identified in European shellfish at relatively low levels, with a lack of sufficient data on occurrence. Studies on shellfish and bacterial strains found no TTX analog in bacteria, despite its presence in shellfish. Survey of bivalves in French production areas revealed fluctuating concentrations of TTX, confirming the complexity of the link between bacteria, bivalves, and TTX.
Article
Geochemistry & Geophysics
Alan N. Heays, Tereza Kaiserova, Paul B. Rimmer, Antonin Knizek, Lukas Petera, Svatopluk Civis, Libor Juha, Roman Dudzak, Manuel Scherf, Helmut Lammer, Robert Pascal, Martin Ferus
Summary: The high-energy-density synthesis of NxOy species was simulated using laser-induced dielectric breakdown (LIDB), and the results showed that the measured yields of the products were in broad agreement with previous experiments and models, except for N2O, which exceeded the previous experimental results and could not contribute to global warming.
JOURNAL OF GEOPHYSICAL RESEARCH-PLANETS
(2022)
Article
Marine & Freshwater Biology
Aurelien Bouquet, Mohamed Laabir, Jean Luc Rolland, Nicolas Chomerat, Christelle Reynes, Robert Sabatier, Christine Felix, Tom Berteau, Claude Chiantella, Eric Abadie
Summary: This study analyzes the water quality contamination in French Mediterranean lagoons and identifies the impact of environmental factors on the abundance of algae and shellfish contamination. It provides useful tools for the sanitary management of shellfish farming areas.
Article
Food Science & Technology
Aurelien Bouquet, Marie Anais Perdrau, Mohamed Laabir, Elodie Foucault, Nicolas Chomerat, Jean Luc Rolland, Eric Abadie
Summary: This study found that PnTX and Prtn toxins accumulated in shellfish can be transferred to the upper food chain through L. ramada fish without impacting their viability.
Article
Pharmacology & Pharmacy
Hicham Aboualaalaa, Mohammed L'Bachir El Kbiach, Benlahcen Rijal Leblad, Fabienne Herve, Amal Hormat-Allah, Lauriane Baudy, Ismail Ennaskhi, Ikram Hammi, Mustapha Ibghi, Hind Elmortaji, Eric Abadie, Jean Luc Rolland, Zouher Amzil, Mohamed Laabir
Summary: Environmental changes in the Mediterranean waters have led to modifications in the phytoplankton populations, especially the Harmful Algal Blooms (HABs) species. This study conducted a one-year monitoring in the western Mediterranean coast of Morocco and measured toxin levels in mollusks. The results suggest the potential emergence of new species producing potent toxins in the southwestern Mediterranean.
Article
Biology
Robert Pascal, Addy Pross
Summary: Recent scientific research suggests that the mental state of life may be a result of its physical state, providing further insights into the physico-chemical process by which life emerged from non-life.
Review
Chemistry, Multidisciplinary
Robert Pascal, Addy Pross
Summary: One of the most remarkable aspects of life is its mental dimension, the origin and essence of which remain a mysterious scientific puzzle. Recent discoveries in microbiology suggest that even the simplest life forms possess highly developed cognitive abilities, possibly indicating that these capabilities emerged early in the evolutionary process. Additionally, the discovery of a new dimension within chemical space of dynamic kinetically stable (DKS) chemical systems offers insights into the emergence of systems with non-physical characteristics, including cognition and self. These findings, combined with a physically based understanding of evolution, provide a possible explanation for the physical/chemical basis of life's mental state and its emergence, potentially advancing our understanding of the "mind from matter" problem.
ISRAEL JOURNAL OF CHEMISTRY
(2023)
Article
Food Science & Technology
Aurelien Bouquet, Christine Felix, Estelle Masseret, Coralie Reymond, Eric Abadie, Mohamed Laabir, Jean Luc Rolland
Summary: In this study, a method using artificial substrates coupled with qPCR was developed to detect V. rugosum in a marine environment. The AS-qPCR method is sensitive, specific, and easy to standardize, and does not require specialized expertise in taxonomy. The method revealed the presence of V. rugosum in French Mediterranean lagoons and proved to be more accurate than light microscopy for monitoring V. rugosum.
Article
Marine & Freshwater Biology
Aurelien Bouquet, Anne Thebault, Nathalie Arnich, Elodie Foucault, Elise Caillard, Camille Gianaroli, Elise Bellamy, Jean Luc Rolland, Mohamed Laabir, Eric Abadie
Summary: This study aimed to predict the human health risks associated with Vulcanodinium rugosum and its neurotoxins in Mediterranean lagoons. The occurrence of V. rugosum was significantly associated with seawater temperature. PnTX G contamination of mussels was highest in summer but persisted throughout the year. The study calculated the human acute dietary exposures and predicted that 25% of people who consume mussels could exceed the provisional acute benchmark value during the warmest periods.
Article
Fisheries
Walid Medhioub, Rafika Challouf, Mohamed Laabir, Youness Limayem, Sondes Bchir, Wissem Slimeni, Dorsaf El Amri, Asma Hamza, Mabrouka Mahfoudhi, Benlahcen Rijal Leblad, Mohamed Nejib Medhioub, Eric Abadie, Jean-Luc Rolland, Mohamed Salah Azaza
Summary: This study investigated the potential exploitation of the brown mussel Perna perna in an aquaculture farm in Monastir Bay, Tunisia. The study determined the quality of seawater and various performance indicators of the mussel over an annual cycle. The results suggest that Monastir Bay's finfish farms could be suitable sites for farming P. perna, as the mussels showed extended sexual cycles and growth rates, and potentially toxic microalgae species developed at low densities in the area.
AQUACULTURE REPORTS
(2023)
Review
Biology
Ziwei Liu, Jean-Christophe Rossi, Robert Pascal
