Article
Biochemistry & Molecular Biology
Johannes Van den Boom, Guendalina Marini, Hemmo Meyer, Helen R. Saibil
Summary: In this study, cryo-EM structures were used to reveal the mechanism of AAA+-ATPase p97 in disassembling a protein phosphatase-1 (PP1) complex. The results showed that PP1 and its associated proteins SDS22 and inhibitor-3 (I3) were tightly loaded onto p97 through direct contact with its N-domain, while the p37 adapter bridged two adjacent p97 N-domains underneath the complex. A portion of I3 was threaded into the central channel of the spiral-shaped p97 hexamer, while the rest remained attached to PP1. These findings elucidate how p97 organizes a protein complex and suggest a hold-and-extract mechanism for p97-mediated disassembly.
Article
Chemistry, Applied
Hekai Zhao, Shengnan Wang, Xiulin Liu, Guilan Zhao, Lina Yang, Hong Song, Guangchen Zhang, Yutang He, He Liu
Summary: The non-covalent complex of soy protein isolate fiber and soy soluble polysaccharide was used to create a pH-responsive emulsion system, demonstrating stability and drug release at different pH values.
Article
Cell Biology
Sahana Shankar, Zi-Ting Hsu, Artur Ezquerra, Chien-Chien Li, Tzu-Lun Huang, Etienne Coyaud, Ricardo Viais, Cedric Grauffel, Brian Raught, Carmay Lim, Jens Luders, Su-Yi Tsai, Kuo-Chiang Hsia
Summary: This study reveals a y-tubulin ring complex (y-TuRC)-dependent pathway that promotes cilia disassembly and prevents cilia formation. It is further demonstrated that Kif2A is recruited to the cilium basal body in a y-TuRC-dependent manner. These findings illustrate the important role of y-TuRC in ciliogenesis.
Article
Chemistry, Multidisciplinary
Jesse A. Jones, Ajitha S. Cristie-David, Michael P. Andreas, Tobias W. Giessen
Summary: Researchers introduced a peptide capable of triggering conformational change in the largest known encapsulin nanocompartment, enabling controlled disassembly and reassembly under physiological conditions. The engineered nanocage demonstrated the ability to encapsulate proteins of choice in vivo and load cargo in vitro, serving as a functionally robust addition to the nanocage toolbox and a novel approach for controlling protein nanocage assembly.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Polymer Science
Catalina Molano-Lopez, Susanne Braun, Michael Kather, Alexander Toepel, Gil van Wissen, Andrij Pich
Summary: This work focuses on the pH-triggered disassembly of supramacromolecular microgels. The properties of the microgels can be easily tuned by varying the ratio between the temperature-responsive poly(N-vinylcaprolactam) and the natural polyphenol tannic acid. The pH and temperature can precisely control the degradation profile of the microgels.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Kyle Decker, Andrew Gould, Patryk Labedz, Justin Rederer, Graham B. Griffin, Sumit Kewalramani, Charles M. Rubert Perez
Summary: This study introduces a new type of amphiphilic molecule, Naph-Ahx-His, capable of self-assembling into stable nano-ribbons in a buffered aqueous solution. These nanoribbons have a crystalline structure and can remain stable in solution for weeks. Due to the presence of a metal chelator histidine, the molecule can disassemble in the presence of high copper concentration. Computational studies were performed to explain the binding affinity preference for copper and its rapid disassembly in aqueous solution. The findings suggest that Naph-Ahx-His nanoribbons have potential applications as metal-triggered nanomaterials.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Cell Biology
Kexin Kang, Qiaoni Shi, Xu Wang, Ye-Guang Chen
Summary: Kang et al. demonstrate that Dvl2 undergoes phase separation and rapidly accumulates to the plasma membrane upon Wnt stimulation. Dvl2 phase separation plays a crucial role in the assembly of the Wnt receptor signalosome and disruption of the beta-catenin destruction complex.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Hyojin Kim, Yaoyao Fu, Ho Jeong Hong, Seong-Gyu Lee, Dong Sun Lee, Ho Min Kim
Summary: Insulin-like growth factors (IGFs) play various roles in embryonic and postnatal growth and differentiation. This study reports the atomic structure of the ternary complex (IGF1/IGF-binding protein3/acid labile subunit) and its assembly/disassembly mechanism for IGF bioavailability, providing insights into human diseases associated with IGF1 and IGFALS gene mutations.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Gan Xu, Hong-Xia Zhang, Xiao-Qiang Li, De-Chao Yang, Jian-Yong Liu
Summary: The study demonstrates a red light-responsive prodrug, BDP-TK-CPT, which combines photodynamic therapy and chemotherapy for anti-tumor effects.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Organic
Yayue Wang, Guohua Shen, Jie Li, Wuyu Mao, Hongbao Sun, Ping Feng, Haoxing Wu
Summary: This study reports the bioorthogonal cleavage of methylene tetrazines with ether or ester linkages in the presence of trans-cyclooctene. This approach allows for the controlled release of molecules with phenol or carboxylic acid moieties and has potential applications in various biomedical fields.
Article
Engineering, Biomedical
Guo-Qiao Wang, Jia Yang, Da-Yong Hou, Rui Zheng, Muhetaerjiang Mamuti, Min-Jie Guo, Zhi Fan, Hong-Wei An, Hao Wang
Summary: A therapeutic peptide modified with bicarbonates on guanidine groups is designed to self-assemble into nanoparticles in the acidic tumor microenvironment, efficiently inhibiting tumor growth and reducing hemolytic toxicity.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Sofia Julin, Nadine Best, Eduardo Anaya-Plaza, Eeva Enlund, Veikko Linko, Mauri A. Kostiainen
Summary: Stimuli-responsive lipid-DNA origami fibers were formed through electrostatic co-assembly of cationic lipids and 6-helix bundle (6HB) DNA origami. The photosensitive lipid degrades under UV-A light and allows controlled release of 6HBs from the fibers.
CHEMICAL COMMUNICATIONS
(2023)
Editorial Material
Cell Biology
Christopher M. Jakobson, Daniel F. Jarosz
Summary: Cells reorganize their contents into subcellular structures in response to stimuli, with new research showing that proteins interact with substrates to coordinate disassembly of cellular structures and directly sense the cellular energy state for adaptive reorganization.
NATURE CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Marika K. Kucinska, Juliette Fedry, Carmela Galli, Diego Morone, Andrea Raimondi, Tatiana Solda, Friedrich Forster, Maurizio Molinari
Summary: This study reveals that expansion of the mammalian ER leads to the disassembly of LINC complexes and swelling of ONM. Upon resolution of ER stress, the physiologic distance between ONM and INM is restored through a catabolic pathway called micro-ONM-phagy.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Deepshikha Verma, Veena Hegde, John Kirkpatrick, Teresa Carlomagno
Summary: The discovery of a fuzzy protein-RNA complex formed by the intrinsically unfolded protein PYM and RNA suggests that specific interactions can occur without well-defined intermolecular interfaces. The first 160 amino acids of PYM (PYM1-160) were shown to be intrinsically disordered and capable of binding RNA independently of its nucleotide sequence. This fuzzy protein-RNA complex down-regulates PYM activity by blocking its interaction with the exon junction complex (EJC), thus enabling PYM to bind to a variety of diverse interaction partners.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Applied
Anindya Basu, Sundaravadanam Vishnu Vadanan, Sierin Lim
CARBOHYDRATE POLYMERS
(2019)
Article
Biochemical Research Methods
Jian Ju, Sagar Regmi, Afu Fu, Sierin Lim, Quan Liu
JOURNAL OF BIOPHOTONICS
(2019)
Article
Chemistry, Physical
Samyukta Ravishankar, Sierin Lim
Article
Biochemical Research Methods
Jiajun Tan, Valeriia Zaremska, Sierin Lim, Wolfgang Knoll, Paolo Pelosi
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Mridul Sarker, Hwankyu Lee, Rui A. Goncalves, Yeng Ming Lam, Haibin Su, Sierin Lim
ADVANCED MATERIALS INTERFACES
(2020)
Article
Chemistry, Multidisciplinary
Samyukta Ravishankar, Seigo Suzuki, Toshiki Sawada, Sierin Lim, Takeshi Serizawa
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2020)
Article
Chemistry, Multidisciplinary
Valeriia Zaremska, Jiajun Tan, Sierin Lim, Wolfgang Knoll, Paolo Pelosi
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Materials Science, Paper & Wood
Thao T. H. Pham, Sundaravadanam Vishnu Vadanan, Sierin Lim
Article
Multidisciplinary Sciences
Luis E. Contreras-Llano, Conary Meyer, Yao Liu, Mridul Sarker, Sierin Lim, Marjorie L. Longo, Cheemeng Tan
NATURE COMMUNICATIONS
(2020)
Article
Plant Sciences
Peter Kitin, Satoshi Nakaba, Christopher G. Hunt, Sierin Lim, Ryo Funada
Article
Multidisciplinary Sciences
Sabyasachi Mukhopadhyay, Senthil Kumar Karuppannan, Cunlan Guo, Jerry A. Fereiro, Adam Bergren, Vineetha Mukundan, Xinkai Qiu, Olga E. Castaneda Ocampo, Xiaoping Chen, Ryan C. Chiechi, Richard McCreery, Israel Pecht, Mordechai Sheves, Rupali Reddy Pasula, Sierin Lim, Christian A. Nijhuis, Ayelet Vilan, David Cahen
Review
Materials Science, Biomaterials
Nicole F. Steinmetz, Sierin Lim, Frank Sainsbury
BIOMATERIALS SCIENCE
(2020)
Article
Biochemical Research Methods
Jiaqing Yu, Guoyun Sun, Nicholas Weikang Lin, Sundaravadanam Vishnu Vadanan, Sierin Lim, Chia-Hung Chen
Article
Pharmacology & Pharmacy
Fatemeh Nasrollahi, Barindra Sana, David Paramelle, Samad Ahadian, Ali Khademhosseini, Sierin Lim
ADVANCED THERAPEUTICS
(2020)
Article
Pharmacology & Pharmacy
Rupali Reddy Pasula, Ambili Kuniyil, Sierin Lim
ADVANCED THERAPEUTICS
(2020)