Article
Biochemistry & Molecular Biology
Haixia Wang, Shumei Wang, Wei Wang, Lin Xu, Lydia R. J. Welsh, Marek Gierlinski, Stephen C. Whisson, Piers A. Hemsley, Petra C. Boevink, Paul R. J. Birch
Summary: Cytoplasmic RXLR effectors from Phytophthora infestans enter plant host cells via clathrin-mediated endocytosis. Transient silencing of clathrin heavy chain or the endosome marker gene attenuated P. infestans infection and reduced translocation of RXLR effectors into host cells. Co-localization of RXLR effectors with clathrin-coated vesicles and immunoprecipitation experiments confirmed the uptake of RXLR effectors via clathrin-mediated endocytosis.
Review
Cell Biology
Petia Adarska, Luis Wong-Dilworth, Francesca Bottanelli
Summary: Molecular switches of the ADP-ribosylation factor (ARF) GTPase family play crucial roles in coordinating intracellular trafficking, including membrane deformation, cargo sorting, and recruitment of downstream coat proteins and effectors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Plant Sciences
Yutaro Shimizu, Tomohiro Uemura
Summary: Membrane trafficking is crucial for organelle maintenance and function, and the TGN plays a critical role as a sorting station. Recent studies have identified distinct subdomains of the TGN for different trafficking pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Azumi Yoshimura, Stephanie Miserey-Lenkei, Evelyne Coudrier, Bruno Goud
Summary: Arp2/3 inhibition affects the acetylated microtubule network and induces abnormal accumulation of long RAB1/GM130-positive carriers, resulting in a decreased rate of anterograde cargo arrival at the Golgi apparatus.
Article
Multidisciplinary Sciences
Sanjoy Paul, Anjon Audhya, Qiang Cui
Summary: The Sar1 GTPase plays a key role in COPII-mediated protein transport by generating membrane curvature on the endoplasmic reticulum. However, the molecular mechanism by which Sar1 is activated and regulates membrane remodeling remains unclear. Using molecular dynamics simulations, we show that Sar1-GTP inserts into the membrane more efficiently and generates higher positive curvature compared to Sar1-GDP. Dimerization of Sar1-GTP further amplifies curvature generation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Alazne Arrazola Sastre, Miriam Luque Montoro, Hadriano M. Lacerda, Francisco Llavero, Jose L. Zugaza
Summary: Rab and Arf GTPases play crucial roles in the pathogenesis and progression of neurodegenerative diseases, making them potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Pallavi V. Vetal, Yves Poirier
Summary: Inorganic phosphate (Pi) homeostasis is crucial for plant growth and relies on the transport of Pi across plant cells. A protein called PHO1 plays a role in this transport process, but its localization on the plasma membrane does not align with its function. By inhibiting a process called clathrin-mediated endocytosis (CME), researchers discovered that PHO1 can be re-localized to the plasma membrane, which affects its transport function.
Article
Cell Biology
Viktoria Szentgyoergyi, Anne Spang
Summary: Cargo delivery in cellular organelles relies on the fusion of vesicles with the help of tethering factors. Recent studies show that tethers play a significant role in membrane fusion, and the discovery of novel tether FERARI complex has changed our understanding of cargo transport.
JOURNAL OF CELL SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Rebecca J. J. Taylor, Grigory Tagiltsev, John A. G. Briggs
Summary: COPI-coated vesicles are involved in transport between Golgi stacks and retrograde transport from the Golgi to the endoplasmic reticulum. This process is regulated by small GTPases of the Arf family, which recruit the COPI coat to the membrane for vesicle formation. Understanding the interplay between coatomer, Arf GTPases, and their effectors is essential to comprehend the mechanism of COPI-coated vesicle trafficking.
Review
Biochemistry & Molecular Biology
Qingchun Lu, Po-Shun Wang, Ling Yang
Summary: This review focuses on highlighting Golgi-associated Rab GTPases as master regulators of autophagic flux, discussing the future research prospects in elucidating their role and mechanism in autophagy and autophagy-related diseases.
CELL AND BIOSCIENCE
(2021)
Article
Cell Biology
Manfei Li, Ran Zhao, Yanfang Du, Xiaomeng Shen, Qiang Ning, Yunfu Li, Dan Liu, Qing Xiong, Zuxin Zhang
Summary: This study characterized the AGAP-recruited complex and its roles in maize cellular physiology and agronomically important traits. The coordinated KNR6-AGAP-ARF1 complex modulates vegetative and reproductive traits by participating in vesicle trafficking in maize, providing insight into the regulation of inflorescence development, ear length, and kernel number in maize.
Article
Multidisciplinary Sciences
Synne Arstad Bjornestad, Noemi Antonella Guadagno, Ingrid Kjos, Cinzia Progida
Summary: In this study, we identified Rab33b as a crucial regulator of cell migration by modulating the delivery of integrins to focal adhesions. Rab33b interacts with Exoc6, a subunit of the exocyst complex, to mediate the postGolgi transport to the plasma membrane. These findings highlight the significance of Rab33b in cellular processes beyond intracellular trafficking.
Review
Biochemistry & Molecular Biology
Antonio Galindo, Sean Munro
Summary: Transport Protein Particle (TRAPP) complexes are conserved complexes that act as nucleotide exchange factors for Rab GTPases. They play roles in protein secretion, autophagy, and other processes. There are two complexes in metazoans: TRAPPII activates Rab11, and TRAPPIII activates Rab1. Crystal and cryo-EM structures have provided insights into the organization and activation mechanisms of these complexes.
Review
Cell Biology
Klaus Ebnet, Volker Gerke
Summary: Membrane polarity, regulated by polarized membrane trafficking and the presence of intramembrane diffusion barriers, is crucial for the development of multicellular tissues. Rho and Rab family small GTPases play important roles in cell-cell junction formation and polarized membrane traffic.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Medicine, Research & Experimental
Jiayi Liu, Xuanjun Zheng, Xiaoqian Wu
Summary: This review summarizes the importance of Rab GTPase in cardiovascular diseases and suggests its potential as a therapeutic target.
Article
Chemistry, Multidisciplinary
Manuela Curcio, Alessandro Paoli, Giuseppe Cirillo, Sebastiano Di Pietro, Martina Forestiero, Francesca Giordano, Loredana Mauro, Diana Amantea, Valeria Di Bussolo, Fiore Pasquale Nicoletta, Francesca Iemma
Summary: This study successfully prepared new dual pH/redox-responsive nanoparticles with affinity for folate receptors, demonstrating potential applicability as a new drug vector in cancer therapy.
Article
Biology
Zackie Aktary, Alejandro Conde-Perez, Florian Rambow, Mathilde Di Marco, Francois Amblard, Ilse Hurbain, Graca Raposo, Cedric Delevoye, Sylvie Coscoy, Lionel Larue
Summary: Dynlt3 plays a crucial role in melanosome transport, maturation, and transfer to keratinocytes, essential for proper skin pigmentation. The Wnt/beta catenin signaling pathway regulates Dynlt3 levels, contributing to the control of melanocyte transport and skin pigmentation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Zoology
Silvia Benito-Martinez, Laura Salavessa, Graca Raposo, Michael S. Marks, Cedric Delevoye
Summary: The synthesis and transfer of melanin in mammalian skin are still debated and poorly understood, but likely play an important role in the photoprotective properties of melanin in the skin.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2021)
Article
Zoology
Linh Le, Julia Sires-Campos, Graca Raposo, Cedric Delevoye, Michael S. Marks
Summary: Melanins, the main pigments of the skin and hair in mammals, are synthesized within membrane-bound organelles of melanocytes called melanosomes. The structure and function of melanosomes are determined by resident transmembrane proteins, defects of which may lead to various forms of albinism. Further investigation is needed to address unanswered questions regarding the molecular mechanisms of melanosome formation and the delivery of specific components.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Florent Figon, Ilse Hurbain, Xavier Heiligenstein, Sylvain Trepout, Arnaud Lanoue, Kadda Medjoubi, Andrea Somogyi, Cedric Delevoye, Graca Raposo, Jerome Casas
Summary: Pigment organelles of vertebrates, represented by melanosomes, belong to the lysosome-related organelle (LRO) family, but their catabolism remains poorly understood. By studying crab spiders, researchers found that pigment organelles share ultrastructural and chemical characteristics with LROs, and their catabolism likely involves intracellular degradation and removal of content through lysosomal mechanisms. This suggests that the endolysosomal system is fully functionalized for within-cell turnover of pigments, supporting the hypothesis of intracellular turnover of pigments originally formulated for human eye melanosomes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Mathilde Bergamelli, Helene Martin, Melinda Benard, Jerome Ausseil, Jean-Michel Mansuy, Ilse Hurbain, Mailys Mouysset, Marion Groussolles, Geraldine Cartron, Yann Tanguy le Gac, Nathalie Moinard, Elsa Suberbielle, Jacques Izopet, Charlotte Tscherning, Graca Raposo, Daniel Gonzalez-Dunia, Gisela D'Angelo, Cecile E. Malnou
Summary: Extracellular vesicles (EVs) play important roles during pregnancy, serving as both potential biomarkers and mediators in communication between the placenta and maternal/fetal sides. Infection can alter the expression levels of surface markers on EVs, while still maintaining their secretion and integrity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Julia Sires-Campos, Ana Lambertos, Cedric Delevoye, Graca Raposo, Dorothy C. Bennett, Elena Sviderskaya, Celia Jimenez-Cervantes, Conchi Olivares, Jose Carlos Garcia-Borron
Summary: The absence of MGRN1 leads to cell-autonomous stimulation of pigment production in melanocytes by increasing tyrosinase activity through neutralizing melanosomal pH. Regulating melanosomal pH may involve several key genes such as subunits of V-ATPase and Mucolipin 3.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ilse Hurbain, Anne-Sophie Mace, Maryse Romao, Elodie Prince, Lucie Sengmanivong, Laurent Ruel, Renata Basto, Pascal P. Therond, Graca Raposo, Gisela D'Angelo
Summary: The microvilli in the epithelium of the wing imaginal disc in Drosophila are responsible for the generation of small extracellular vesicles (EVs) that transport the morphogen Hedgehog (Hh) across the tissue. The integrity of microvilli is maintained by the Prominin-like (PromL) protein, which is crucial for its secretory function. Importantly, the distribution of Hh to microvilli and its release via EVs contribute to the proper morphogenesis of the wing imaginal disc.
Editorial Material
Medicine, Research & Experimental
Chantal M. Boulanger, Graca Raposo, Clotilde Thery
M S-MEDECINE SCIENCES
(2021)
Article
Nutrition & Dietetics
Sabrina Bossio, Anna Perri, Rocco Malivindi, Francesca Giordano, Vittoria Rago, Maria Mirabelli, Alessandro Salatino, Antonio Brunetti, Emanuela Alessandra Greco, Antonio Aversa
Summary: Recent studies have shown that oleuropein (OLE) found in olive leaf extract can inhibit tumor cell proliferation and reduce the invasiveness of cancer cells. It achieves this by modulating cell-cycle regulators, promoting apoptosis, and inhibiting cell migration and motility. However, further research is needed to understand the specific mechanisms involved, especially in seminoma cells.
Article
Biochemistry & Molecular Biology
Luca Gelsomino, Ines Barone, Amanda Caruso, Francesca Giordano, Matteo Brindisi, Giovanna Morello, Felice Maria Accattatis, Salvatore Panza, Anna Rita Cappello, Daniela Bonofiglio, Sebastiano Ando, Stefania Catalano, Cinzia Giordano
Summary: Tumor extracellular vesicles (EVs) play a crucial role in cell-to-cell communication in breast cancer by transporting nucleic acids, proteins, and metabolites. Leptin induces the release of EVs from breast cancer cells, and these vesicles have a specific proteomic signature related to mitochondrial processes and activity. Lep-EVs increase ATP levels and modulate the oxidative metabolism of breast cancer cells. Furthermore, Lep-EVs sustain the polarization of macrophages in the tumor microenvironment (TME) towards a tumor-promoting phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Giuseppina Daniela Naimo, Alessandro Paoli, Francesca Giordano, Martina Forestiero, Maria Luisa Panno, Sebastiano Ando, Loredana Mauro
Summary: Obesity plays an important role in the initiation and progression of breast tumors, mainly through the development of chronic low-grade inflammation and dysfunction in adipose tissue biology. AdipoRs, belonging to the seven-transmembrane receptor family, mediate the effect of adiponectin on breast cancer cell proliferation, which is reduced in obesity. The adiponectin/AdipoRs axis is becoming increasingly important as a therapeutic target for breast cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Graca Raposo, Guillaume van Niel, Philip D. Stahl
Summary: FBA BioAdvances has created a special collections category called FBA special collections (FBA SC) focusing on emerging disciplines such as extracellular vesicles (EVs) and homeostasis. Global leading investigators have contributed articles covering a range of research activities related to EVs biology.