Article
Cell Biology
Jianshi Yu, Mariarita Perri, Jace W. Jones, Keely Pierzchalski, Natalia Ceaicovscaia, Erika Cione, Maureen A. Kane
Summary: The study investigated the relationship between RBP1 expression and atRA, finding that RBP1 and atRA are reduced in mammary tumor tissue and tumorigenic epithelial cell lines. Increasing atRA levels could activate direct atRA targets, inhibit proliferation, and collagen deposition. Conditions in tumor microenvironments reduced RBP1 expression and atRA, while treatments with specific agonists or agents could restore RBP1 expression and atRA levels. This work highlights the potential therapeutic benefit of restoring RBP1 expression and endogenous atRA in diseases with reduced RBP1.
Article
Multidisciplinary Sciences
Yeon-Woo Cho, Seohyeon Jee, Intan Rosalina Suhito, Jeong-Hyeon Lee, Chun Gwon Park, Kyung Min Choi, Tae-Hyung Kim
Summary: This study reports a new platform called SMENA, which enables efficient generation of neuronal cells using single metal-organic framework nanoparticles embedded in nanopit arrays. The platform allows for long-term storage and release of retinoic acid, leading to enhanced mRNA expressions of neurogenesis-related activities in neural stem cells.
Editorial Material
Biology
J. Christian Althaus, Michael A. Sutton
Summary: All-trans retinoic acid induces synaptic plasticity in human cortical circuits by engaging the spine apparatus, leading to structural and functional changes at synapses.
Article
Biochemistry & Molecular Biology
Qianqian Jin, Chuncao Huo, Wenhao Yang, Kaidi Jin, Shuai Cai, Yanxin Zheng, Baoyu Huang, Lei Wei, Meiwei Zhang, Yijing Han, Xuekai Zhang, Yaqiong Liu, Xiaotong Wang
Summary: This study investigates the effect of all-trans retinoic acid (ATRA) on the transcriptome of the Pacific oyster Crassostera gigas. A total of 586 differentially expressed genes (DEGs) were identified, with ATRA affecting the development, metabolism, reproduction, and immunity of C. gigas. Several tyrosinase genes and Tyr-related genes were upregulated by ATRA, and the DNA-binding activity of the RA receptors CgRAR and CgRXR, as well as their interaction with RAREs in the Tyr-2 promoter, were confirmed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yu-Lung Lin, Jennifer Nhieu, Pei-Yao Liu, Gengyun Le, Dong Jun Lee, Chin-Wen Wei, Yi-Wei Lin, Sang-Hyun Oh, Dawn Lowe, Li-Na Wei
Summary: This study identifies the role of cellular retinoic acid-binding protein 1 (CRABP1) in the formation and maintenance of the neuromuscular junction (NMJ) and highlights the regulatory role of CRABP1-CaMKII-Agrn signaling in NMJ. It also suggests a potential protective role of CRABP1 in MN diseases.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Ramesh M. Chingle, Masahiko Imai, Sarah Altman, Daisuke Saito, Noriko Takahashi, Terrence R. Burke
Summary: Retinoic acid binds to retinoic acid receptors to regulate gene expression and has important functions such as cell proliferation and differentiation. A new p-dodecylaminophenol derivative, without side effects or toxicity and effective against a wide range of cancers, has been developed. Introducing the carboxylic acid motif found in retinoids potentially enhances the anti-proliferative effects.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Xi Lai, Qian Zhang, Jiang Zhu, Ting Yang, Min Guo, Qiu Li, Huan Liu, Qiong-Hui Wu, Jie Chen, Ting-Yu Li
Summary: This study compared two vitamin A supplementation programs for children with ASD and VAD in China, finding that a weekly dose of vitamin A was more effective in increasing vitamin A levels and improving social functioning.
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2021)
Review
Physiology
Anqun Chen, Yu Liu, Yu Lu, Kyung Lee, John Cijiang He
Summary: Retinoic acid (RA) has dual effects in kidney disease, providing renal protection while also potentially inducing podocyte injury and apoptosis, highlighting the need to target specific RA-mediated pathways for effective therapeutic treatments.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Qi-Wen Wang, Ding-Jia-Cheng Jia, Jia-Min He, Yong Sun, Yun Qian, Qi-Wei Ge, Ya-Dong Qi, Qing-Yi Wang, Ying-Ying Hu, Lan Wang, Yan-Fei Fang, Hui-Qin He, Man Luo, Li-Jun Feng, Jian-Min Si, Zhang-Fa Song, Liang-Jing Wang, Shu-Jie Chen
Summary: L. intestinalis has a protective effect against ulcerative colitis by modulating the interaction between Th17 cells and gut epithelial cells. It inhibits Th17 cell differentiation by reducing the production of C/EBPA-driven SAA1 and SAA2, thus attenuating colitis progression.
Article
Genetics & Heredity
Veronique Caron, Nicolas Chassaing, Nicola Ragge, Felix Boschann, Angelina My-Hoa Ngu, Elisabeth Meloche, Sarah Chor, Saquib A. Lakhani, Weizhen Ji, Laurie Steiner, Julien Marcadier, Philip R. Jansen, Laura A. van de Pol, Johanna M. van Hagen, Alvaro Serrano Russi, Gwenael Le Guyader, Magnus Nordenskjold, Ann Nordgren, Britt-Marie Anderlid, Julie Plaisancie, Corinna Stoltenburg, Denise Horn, Anne Drenckhahn, Fadi F. Hamdan, Mathilde Lefebvre, Tania Attie-Bitach, Peggy Forey, Vasily Smirnov, Francoise Ernould, Marie-Line Jacquemont, Sarah Grotto, Alberto Alcantud, Alicia Coret, Rosario Ferrer-Avargues, Siddharth Srivastava, Catherine Vincent-Delorme, Shelby Romoser, Nicole Safina, Dimah Saade, James R. Lupski, Daniel G. Calame, David Genevieve, Nicolas Chatron, Caroline Schluth-Bolard, Kenneth A. Myers, William B. Dobyns, Patrick Calvas, Caroline Salmon, Richard Holt, Frances Elmslie, Marc Allaire, Daniil M. Prigozhin, Andre Tremblay, Jacques L. Michaud
Summary: This study reports 25 individuals with pathogenic or likely pathogenic variants in the RARB gene, aiming to characterize their functional impact and the clinical spectrum of MCOPS12. The findings show that all tested RARB variants exhibit either gain-of-function or loss-of-function activity, leading to disrupted RARB function and variable clinical manifestations associated with MCOPS12.
GENETICS IN MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Geoffrey Brown
Summary: All-trans retinoic acid plays a role in embryogenesis and also acts as a teratogen. The deregulation of cytosolic aldehyde dehydrogenases in various human cancers affects the synthesis of all-trans retinoic acid. Inhibiting these enzymes reduces cancer cell proliferation, induces apoptosis, and enhances chemotherapeutic sensitivity. RAR gamma is an oncogene in several types of cancer, and inhibiting its action leads to cancer cell death. Targeting the retinoic acid pathway shows promise in eliminating cancer stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Trey Polvadore, Malcolm Maden
Summary: This study reveals the impact of retinoic acid treatment on the control of positional information during axolotl limb regeneration. Through RNA sequencing, the genes interacting with RAR alpha were identified, shedding light on the mechanism of action for future research.
Article
Cell Biology
Fathima N. Cassim Bawa, Raja Gopoju, Yanyong Xu, Shuwei Hu, Yingdong Zhu, Shaoru Chen, Kavita Jadhav, Yanqiao Zhang
Summary: The study reveals the important role of macrophage retinoic acid receptor alpha (RARα) in regulating atherosclerosis. It protects against atherosclerosis by inducing cholesterol efflux and inhibiting inflammation.
Review
Nutrition & Dietetics
Xiangrong Guo, Hui Wang, Jian Xu, Hui Hua
Summary: Vitamin A is crucial for brain function and has important roles in vision, immunity, and reproduction. Previous research has shown that retinoic acid, the active form of vitamin A, plays a role in regulating biological rhythms. However, limited evidence exists regarding the impact of vitamin A deficiency on biological rhythms, with most studies conducted on animals. This review explores the physiological functions of biological rhythms, the pathways/mechanisms involved in regulating these rhythms, and the associations between vitamin A deficiency and biological rhythm disorders/diseases.
FRONTIERS IN NUTRITION
(2022)
Article
Oncology
John-William Sidhom, Ingharan J. Siddarthan, Bo-Shiun Lai, Adam Luo, Bryan C. Hambley, Jennifer Bynum, Amy S. Duffield, Michael B. Streiff, Alison R. Moliterno, Philip Imus, Christian B. Gocke, Lukasz P. Gondek, Amy E. DeZern, Alexander S. Baras, Thomas Kickler, Mark J. Levis, Eugene Shenderov
Summary: The study demonstrates that deep learning pattern recognition can assist in identifying acute promyelocytic leukemia (APL) with greater discriminatory power and consistency compared to humans. This method not only distinguishes APL effectively, but also extracts previously undescribed morphological features of APL.
NPJ PRECISION ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Stephanie J. Shiffka, Jace W. Jones, Linhao Li, Ann M. Farese, Thomas J. MacVittie, Hongbing Wang, Peter W. Swaan, Maureen A. Kane
JOURNAL OF LIPID RESEARCH
(2020)
Article
Cell & Tissue Engineering
Manasa P. Srikanth, Jace W. Jones, Maureen Kane, Ola Awad, Tea Soon Park, Elias T. Zambidis, Ricardo A. Feldman
Summary: Gaucher disease is caused by mutations in the GBA1 gene, leading to the accumulation of GlcSph which activates an mTORC1-dependent pathogenic mechanism in neurons. Inhibition of acid ceramidase can prevent mTOR hyperactivity and lysosomal dysfunction, suggesting a potential therapeutic target for GBA1-associated neurodegeneration. Incubation with exogenous GlcSph in wild-type neurons can recapitulate the abnormalities observed in mutant neurons, highlighting the role of GlcSph in the pathogenesis of Gaucher disease.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biochemical Research Methods
Anh Tran, I. Abrrey Monreal, Eugene Moskovets, Hector C. Aguilar, Jace W. Jones
Summary: An unmet need exists for analytical strategies that can characterize the lipid composition of viral envelopes and do so on a high-throughput scale. The use of atmospheric pressure MALDI with lithium adduct consolidation allows for rapid and confident profiling of total lipid extracts from enveloped viruses, specifically geared towards sphingolipid detection. This approach provides a robust and high-throughput lipid detection platform for structural characterization of lipid extracts from viruses.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Medicine, Research & Experimental
Ni Yang, Lauren E. Parker, Jianshi Yu, Jace W. Jones, Ting Liu, Kyriakos N. Papanicolaou, C. Conover Talbot, Kenneth B. Margulies, Brian O'Rourke, Maureen A. Kane, D. Brian Foster
Summary: The study showed that there is a decline in cardiac ATRA in heart failure, despite sufficient reserves of cardiac vitamin A. Strategies to normalize ATRA metabolism, such as CYP26 inhibition, may have therapeutic potential in preventing cardiac remodeling and functional decline. This suggests that low cardiac ATRA attenuates critical gene expression programs in heart failure.
Article
Chemistry, Analytical
Yulemni Morel, Nivedita Hegdekar, Chinmoy Sarkar, Marta M. Lipinski, Maureen A. Kane, Jace W. Jones
Summary: The study developed a targeted, quantitative method using HILIC separation and selective reaction monitoring mass spectrometry for specific and sensitive quantitation of 100 PE-P structures. Application of this assay to a TBI mouse model demonstrated distinct temporal profiles for plasma PE-P up to 28 days post injury, showing a significant increase 24 hours after induced TBI followed by a gradual reduction to sham concentrations by day 28.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Carballal, Victor Vitvitsky, Roshan Kumar, David A. Hanna, Marouane Libiad, Aditi Gupta, Jace W. Jones, Ruma Banerjee
Summary: This study demonstrates that H2S increases lipid synthesis in cells by affecting mitochondrial NAD(P)H pools and enabling reductive carboxylation of alpha-ketoglutarate. H2S also leads to time-dependent changes in various lipid classes, upregulating triglycerides while downregulating phosphatidylcholine.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Ludovic Muller, Weiliang Huang, Jace W. Jones, Ann M. Farese, Thomas J. MacVittie, Maureen A. Kane
Summary: Radiation exposure leads to dysregulation of lipid and protein composition in the mesenteric lymph node (MLN), affecting pathways regulating lipids and acute phase response. The study demonstrates molecular alterations in MLN after acute radiation exposure, potentially informing on injury mechanism in a non-human primate model of acute radiation syndrome of the gastrointestinal tract.
Article
Biochemistry & Molecular Biology
Owen B. Becette, Anh Tran, Jace W. Jones, John P. Marino, Robert G. Brinson
Summary: Nucleic acids are a popular platform for developing biotherapeutics. There are currently 14 short oligonucleotide therapeutics and 2 mRNA vaccines approved by the FDA. However, there is a lack of high-resolution structural techniques for characterizing these molecules. In this study, nuclear magnetic resonance (NMR) methods were used to structurally fingerprint short oligonucleotide therapeutics, demonstrating their utility and broad applicability.
NUCLEIC ACID THERAPEUTICS
(2022)
Article
Cell Biology
Jianshi Yu, Mariarita Perri, Jace W. Jones, Keely Pierzchalski, Natalia Ceaicovscaia, Erika Cione, Maureen A. Kane
Summary: The study investigated the relationship between RBP1 expression and atRA, finding that RBP1 and atRA are reduced in mammary tumor tissue and tumorigenic epithelial cell lines. Increasing atRA levels could activate direct atRA targets, inhibit proliferation, and collagen deposition. Conditions in tumor microenvironments reduced RBP1 expression and atRA, while treatments with specific agonists or agents could restore RBP1 expression and atRA levels. This work highlights the potential therapeutic benefit of restoring RBP1 expression and endogenous atRA in diseases with reduced RBP1.
Article
Substance Abuse
Bryan Mackowiak, Mingjiang Xu, Yuhong Lin, Yukun Guan, Wonhyo Seo, Ruixue Ren, Dechun Feng, Jace W. Jones, Hongbing Wang, Bin Gao
Summary: The study found that mice chronically fed with ethanol responded less severely to multiple binge ethanol consumption compared to a single binge. The upregulation of the Cyp2b10 gene after binge ethanol intake can indirectly modify ethanol metabolism and reduce acute-on-chronic ethanol-induced liver injury.
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
(2022)
Article
Physics, Atomic, Molecular & Chemical
Owen B. Becette, Anh Tran, John P. Marino, Jace W. Jones, Robert G. Brinson
Summary: In this study, a simplified and quick method for identification and assessment of a known impurity in nucleic acid drugs was developed using lithium adduct consolidation combined with mass spectrometry and nuclear magnetic resonance spectroscopy. The method is believed to have broad applicability in academic and industrial laboratories.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Genetics & Heredity
Clorissa L. Washington-Hughes, Shubhrajit Roy, Herana Kamal Seneviratne, Senthilkumar S. Karuppagounder, Yulemni Morel, Jace W. Jones, Alex Zak, Tong Xiao, Tatiana N. Boronina, Robert N. Cole, Namandje N. Bumpus, Christopher J. Chang, Ted M. Dawson, Svetlana Lutsenko
Summary: This study reveals the importance of Cu transporter Atp7b in maintaining Cu homeostasis in the brain during postnatal development. Loss of Atp7b function leads to morphological changes in choroid plexus and affects Cu transporters Atp7a and Slc31a, resulting in Cu deficit and associated imbalance of catecholamines and lipids. These findings contribute to our understanding of the neuropathology of Wilson disease.
Article
Biochemical Research Methods
Yulemni Morel, Jace W. Jones
Summary: Lipid peroxidation is crucial in the development of various diseases, as it causes oxidative damage to lipids, leading to dysfunction and death of cells. This study presents an analytical approach for characterizing the structure of oxidized products of specific types of phospholipids, providing insights into the initial impact of lipid peroxidation on cellular membranes and their role in redox biology.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Stephanie Zalesak-Kravec, Weiliang Huang, Jace W. Jones, Jianshi Yu, Jenna Alloush, Amy E. Defnet, Alexander R. Moise, Maureen A. Kane
Summary: This study investigated the impact of CRBP1 loss on the adult mouse heart using a multi-omics approach. The results showed that RA levels were decreased in Rbp1(-/-) mouse heart tissue, and metabolomics and proteomics analyses revealed disrupted pathways related to cellular metabolism and cardiac metabolism. These findings characterize the effect of CRBP1 loss and reduced RA in the adult heart.
Article
Biochemical Research Methods
Anh Tran, Liting Wan, Zhenbo Xu, Janette M. Haro, Bing Li, Jace W. Jones
Summary: The research team utilized lithium adduct consolidation to enhance mass-spectrometry-based sphingolipid analysis and implemented an optimized MALDI matrix system for high-throughput detection. They successfully applied the technology to detect differentially expressed sphingolipids in liver, brain, and plasma matrices, validating the method's efficacy.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)