Article
Fisheries
Vitaliy Kholodnyy, Kiko Shibata, Atife Tuba Beken, Borys Dzyuba, Hermes Bloomfield-Gadelha, Jacky Cosson, Sergii Boryshpolets
Summary: During evolution, fish have adopted diverse spawning tactics to effectively utilize sperm competition and prevent the fertilization of inappropriate male gametes. This study observed that common carps exhibit different spawning behavior compared to salmonids, and the presence of ovarian fluid (OF) altered the motility pattern and velocity of carp spermatozoa. The chemotactic test showed that carp spermatozoa responded significantly to OF, and this attraction phenomenon depended on the presence of external calcium ions. These findings highlight the significant impact of environmental conditions, particularly the presence of OF, on the performance of male gametes in common carp, aligning with their specific spawning behavior.
Article
Biochemistry & Molecular Biology
Kogiku Shiba, Kazuo Inaba
Summary: Spermatozoa can sense and respond to environmental signals to ensure successful fertilization. In the ascidian Ciona intestinalis, CNG channels play a vital role in regulating sperm motility and intracellular calcium regulation during chemotaxis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Mirjam I. Binner, Anna Kogan, Karin Panser, Alexander Schleiffer, Victoria E. Deneke, Andrea Pauli
Summary: Fertilization is a crucial process in sexual reproduction, but the molecular mechanisms behind it are still unclear. A study on zebrafish reveals that Spaca6, a conserved protein, is essential for sperm-egg binding and fusion. The research also uncovers a previously unknown dependence of Dcst2 expression on Spaca6 in sperm.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Soledad N. Gonzalez, Valeria Sulzyk, Mariana Weigel Munoz, Patricia S. Cuasnicu
Summary: Studies on the CRISP protein family in mammalian fertilization have provided insights into their essential roles in gamete interaction and male fertility. Knockout experiments with different CRISP genes revealed their involvement in both gamete interaction and subsequent processes such as sperm transport and early embryo development within the female reproductive tract.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ludmila Curci, Guillermo Carvajal, Valeria Sulzyk, Soledad Natalia Gonzalez, Patricia S. Cuasnicu
Summary: Inactivation of the sperm CatSper channel affects sperm fertilizing ability and function, but does not impact in vitro fertilization. Exposure to HC prior to fertilization significantly reduces in vivo fertilization, indicating the potential for developing a non-hormonal contraceptive by blocking CatSper activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yoshitaka Fujihara, Sarah Herberg, Andreas Blaha, Karin Panser, Kiyonori Kobayashi, Tamara Larasati, Maria Novatchkova, Hans-Christian Theussl, Olga Olszanska, Masahito Ikawa, Andrea Pauli
Summary: Fertilization is a crucial process initiating new individual development in sexually reproducing species. SPACA4, a sperm protein, is essential for efficient fertilization in mammals like mice, particularly for interactions with the zona pellucida. Its absence leads to subfertility in male knockout mice, with fertilization rescue observed when the zona pellucida is removed during in vitro experiments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Shaogeng Tang, Yonggang Luc, Will M. Skinnere, Mrinmoy Sanyala, Polina V. Lishko, Masahito Ikawa, Peter S. Kim
Summary: A study finds that knockout of Tmem95 gene causes male sterility in mice. Using a model experiment, it is discovered that human TMEM95 can bind to hamster egg membranes, and a potential receptor-binding surface region is revealed. Further experiments show that the specific, receptor-mediated interaction between sperm TMEM95 and eggs plays a crucial role in membrane fusion during fertilization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Masahiro Naruse, Midori Matsumoto
Summary: This study elucidated the molecular mechanisms and biophysical properties of sperm chemotaxis in echinoderms. By reconstructing a sperm model in silico, the importance of desensitizing the chemoattractant receptor in long-range chemotaxis was demonstrated. Simulation results showed that desensitization is essential for sperm to approach distantly located eggs. By comparing the model with starfish sperm, the different desensitization schemes and responses to disturbances were analyzed, highlighting the potential application of this approach as a novel tool in chemotaxis research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Ingrid Fliniaux, Guillaume Marchand, Caroline Molinaro, Mathieu Decloquement, Alain Martoriati, Matthieu Marin, Jean-Francois Bodart, Anne Harduin-Lepers, Katia Cailliau
Summary: Sialic acids are a group of monosaccharides with unique physicochemical properties that modulate the biological functions of molecules they are associated with. They play a role in various stages of the reproductive process, particularly in the formation and binding of gametes. Sialoglycoproteins form a specific coat on the surface of gametes, and marine species oocytes have specific polysialylated chains. Spermatozoa undergo crucial changes in sialic acid in the female reproductive tract, facilitating migration, survival, and binding with the oocyte.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yonggang Lu, Kentaro Shimada, Shaogeng Tang, Jingjing Zhang, Yo Ogawa, Taichi Noda, Hiroki Shibuya, Masahito Ikawa
Summary: The knockout of 1700029I15Rik impairs the biosynthesis of acrosomal membrane proteins and leads to subfertility in male mice. 1700029I15Rik interacts with proteins involved in protein processing, glycosylation, and protein trafficking. Its absence destabilizes the non-catalytic subunits of the OST complex, resulting in decreased abundance of acrosomal membrane proteins and impaired sperm-egg fusion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Vitaliy Kholodnyy, Borys Dzyuba, Marek Rodina, Hermes Bloomfield-Gadelha, Manabu Yoshida, Jacky Cosson, Sergii Boryshpolets
Summary: The fertilization of freshwater fish can be negatively affected by environmental factors, with egg and ovarian fluid playing a significant role in triggering gamete encounters. The ovarian fluid of rainbow trout affects sperm performance by inducing a chemotaxis-like effect on activated male gametes, creating an optimal environment for sperm performance. The individual interaction between egg (ovarian fluid) and sperm reflects specific features of the spawning process in this species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vitaliy Kholodnyy, Borys Dzyuba, Hermes Gadelha, Jacky Cosson, Sergii Boryshpolets
Summary: The study explored the effects of ovarian fluid and egg-released substances on sperm behavior in sterlet. Results showed that ovarian fluid inhibited sperm motility initiation, while egg-conditioned medium attracted sperm. The presence of ovarian fluid preserved the fertilizing ability of eggs and extended the period of sperm fertilization.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Review
Cell Biology
Katherine A. Maniates, Andrew Singson
Summary: Complementary forward and reverse genetic approaches have led to a recent surge in the discovery of fertilization genes in various model systems. The number of genetically validated gamete surface molecules has more than doubled in the past few years, with all sperm fertilization genes encoding transmembrane or secreted molecules. However, the discovery of genes encoding oocyte molecules has lagged behind. This review discusses potential experimental biases and biological reasons that may have hindered the identification of egg fertilization genes, as well as current strategies to overcome these obstacles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Alberto M. Rivera, Willie J. Swanson
Summary: Gene duplication and repeat domain expansions play an important role in the evolution of fertilization proteins, with gene duplication often facilitating the cooption of molecules into fertilization function and repeat domain expansions providing opportunities for the evolution of novel fertilization proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Juha Karjalainen, Mikko Makinen, Anna K. Karjalainen
Summary: Sulfate's toxicity to whitefish early development primarily affects fertilization and early embryonic development stages, with decreased fertilization success and embryo survival in high sulfate concentrations. However, the tolerance of whitefish embryos and larvae to sulfate exposure is relatively high, with no significant differences in survival observed between control and sulfate treatments during late embryonic and larval periods. The LC50 values for sulfate indicate its relatively moderate toxicity to whitefish early development stages, comparable to other salmonids.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Yukiko Ogino, Shigehiro Kuraku, Hiroshi Ishibashi, Hitoshi Miyakawa, Eri Sumiya, Shinichi Miyagawa, Hajime Matsubara, Gen Yamada, Michael E. Baker, Taisen Iguchi
MOLECULAR BIOLOGY AND EVOLUTION
(2016)
Article
Fisheries
T. Mueller, H. Matsubara, Y. Kubara, A. Horvath, J. F. Asturiano, B. Urbanyi
JOURNAL OF APPLIED ICHTHYOLOGY
(2017)
Review
Biochemistry & Molecular Biology
Yukiko Ogino, Saki Tohyama, Satomi Kohno, Kenji Toyota, Gen Yamada, Ryohei Yatsu, Tohru Kobayashi, Norihisa Tatarazako, Tomomi Sato, Hajime Matsubara, Anke Lange, Charles R. Tyler, Yoshinao Katsu, Taisen Iguchi, Shinichi Miyagawa
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2018)
Article
Reproductive Biology
Tamas Mueller, Hajime Matsubara, Yuki Kubara, Akos Horvath, Balazs Kolics, Janos Taller, Viktor Steger, Balazs Kovacs, Laszlo Horvath, Juan F. Asturiano, David S. Penaranda, Bela Urbanyi
Article
Fisheries
T. Tomita, T. Kawai, H. Matsubara, R. Nagata
JOURNAL OF FISH BIOLOGY
(2013)
Article
Fisheries
T. Tomita, T. Kawai, H. Matsubara, M. Kobayashi, S. Katakura
JOURNAL OF FISH BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Sean L. Divers, H. James McQuillan, Hajime Matsubara, Takashi Todo, P. Mark Lokman
JOURNAL OF LIPID RESEARCH
(2010)
Article
Biochemistry & Molecular Biology
Yukinori Kazeto, Ryota Tosaka, Hajime Matsubara, Shigeho Ijiri, Shinji Adachi
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2011)
Article
Endocrinology & Metabolism
Hirohiko Kagawa, Takafumi Kishi, Koichiro Gen, Yukinori Kazeto, Ryota Tosaka, Hajime Matsubara, Takahiro Matsubara, Sayumi Sawaguchi
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2011)
Article
Food Science & Technology
Mizuki Sasaki, Hirotaka Katahira, Mari Kobayashi, Toshiaki Kuramochi, Hajime Matsubara, Minoru Nakao
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2019)
Article
Environmental Sciences
Mohamed I. Zanaty, Niina Sawada, Yoichiro Kitani, Hossam F. Nassar, Hamada M. Mahmoud, Kazuichi Hayakawa, Toshio Sekiguchi, Shouzo Ogiso, Yoshiaki Tabuchi, Makoto Urata, Hajime Matsubara, Yutaka Takeuchi, Atsuhiko Hattori, Ajai K. Srivastav, Thumronk Amornsakun, Nobuo Suzuki
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Fisheries
Hajime Matsubara, P. Mark Lokman, Yukinori Kazeto, Hiromi Okumura, Shigeho Ijiri, Toshiaki Hirai, Graham Young, Shinji Adachi, Kohei Yamauchi
Article
Marine & Freshwater Biology
Takashi Takagi, Toshio Sekiguchi, Yuichi Sasayama, Atsuhiko Hattori, Takushi X. Watanabe, Yoichiro Kitani, Yoshiaki Tabuchi, Hajime Matsubara, Ajai K. Srivastav, Tran Ngoc Hai, Thumronk Amornsakun, Nobuo Suzuki
INTERNATIONAL AQUATIC RESEARCH
(2019)
Article
Reproductive Biology
Ryuzo Yanagimachi, Tatsuo Harumi, Hajime Matsubara, Wei Yan, Shuiqiao Yuan, Noritaka Hirohashi, Tomohiro Iida, Etsuro Yamaha, Katsutoshi Arai, Takahiro Matsubara, Tadashi Andoh, Carol Vines, Gary N. Cherr
BIOLOGY OF REPRODUCTION
(2017)