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Biochemistry & Molecular Biology
Lu-Yang Jin, Jia-En Yu, Hai-Yan Xu, Bin Chen, Qian Yang, Ye Liu, Meng-Xi Guo, Cheng-Liang Zhou, Yi Cheng, Hai-Yan Pang, Hai-Yan Wu, Jian-Zhong Sheng, He-Feng Huang
Summary: This study identified the potential role of the Pde4d gene in polycystic ovary syndrome. Experimental validation showed that overexpression of Pde4d led to the formation of ovarian cysts and inhibited follicle maturation and apoptosis. This finding is important for understanding the pathogenesis of polycystic ovary syndrome.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Endocrinology & Metabolism
Marah Armouti, Miriam Rodriguez-Esquivel, Carlos Stocco
Summary: This study investigates the molecular mechanisms of Sik in inhibiting FSH actions in primary rat granulosa cells (GCs), and finds that SIK inhibition enhances the activation of the FSH signaling pathway. It also suggests that SIKs regulate the FSH pathway by affecting factors between cAMP/PKA and CREB.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Reproductive Biology
Kenji Vann, Adelaide E. Weidner, Ariana C. Walczyk, Olga Astapova
Summary: Granulosa cell-specific knockout of adaptor protein paxillin in mice increases fecundity and may reduce granulosa cell death. Knockout of paxillin in granulosa cells leads to increased fertility in older reproductive age mice, which is not due to increased numbers of estrous cycles, ovulated oocytes per cycle, or pups per litter, but rather shorter time to pregnancy and increased number of litters.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biology
Min Chen, Fangfang Dong, Min Chen, Zhiming Shen, Haowei Wu, Changhuo Cen, Xiuhong Cui, Shilai Bao, Fei Gao
Summary: PRMT5 plays a crucial role in follicle development by regulating WT1 expression and facilitating the translation of Wt1 mRNA through methylation of HnRNPA1. It is essential for granulosa cell lineage maintenance, follicle structure, and steroidogenesis-related gene expression.
Article
Biotechnology & Applied Microbiology
Chang Liu, Linshuang Li, Bo Yang, Yiqing Zhao, Xiyuan Dong, Lixia Zhu, Xinling Ren, Bo Huang, Jing Yue, Lei Jin, Hanwang Zhang, Lan Wang
Summary: This study examined the role of N6-methyladenine (m(6)A) methylation in aging-related ovarian reserve decline. The researchers performed a transcriptome-wide profiling of m(6)A methylation in the ovarian granulosa cells of younger and older women. They found that m(6)A methylation was highly conserved and enriched in specific regions of the mRNA. The older group had a higher number of m(6)A methylated genes. Further analysis revealed the enrichment of these methylated genes in various signaling pathways. Additionally, several genes were found to be differently expressed and modified by m(6)A in the aging ovary.
Article
Oncology
Audrey J. Gaskins, Robert B. Hood, Jennifer B. Ford, Russ Hauser, Anna K. Knight, Alicia K. Smith, Todd M. Everson
Summary: This study investigated the relationship between traffic-related air pollution (TRAP) exposure and supplemental folic acid intake with epigenetic aging and CpG-specific DNA methylation in granulosa cells (GC). The results showed associations between TRAP exposure and folic acid intake with DNA methylation sites, but no significant effects on epigenetic aging of GC. These findings suggest that differential DNA methylation may be a potential mechanism underlying the effects of TRAP and folic acid on ovarian function.
CLINICAL EPIGENETICS
(2023)
Article
Endocrinology & Metabolism
Yan Deng, Hu Li, Yi Song, Juan Cen, Yuying Zhang, Yi Sui, Dexuan Cui, Tin Chiu Li, Yan Xu, Chi Chiu Wang, Pui Wah Jacqueline Chung, Tao Tang
Summary: This study investigated the lncRNA and mRNA profiles in ovarian granulosa cells from women with PCOS and identified DLGAP5 as a dysregulated candidate gene involved in the pathogenesis of PCOS, particularly in granulosa cell apoptosis and proliferation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
IrantzuADD F. B. Perez-Ruiz, Jose-Ignacio Ruiz-Sanz, Maria-Luisa Hernandez, Rosaura Navarro, Marcos Ferrando, Zaloa Larreategui, Maria-Begona Ruiz-Larrea
Summary: This study provided the first evidence of the presence of mRNAs and proteins of the three members of the PON family in human ovarian granulosa cells, with active secretion of PON1 and PON3 by the cells. Additionally, it was discovered that PON2 mainly localized with mitochondria and the subcellular nucleo-cytoplasmic distribution of PON1 was associated with the cell cycle.
Article
Developmental Biology
Melanie Kaseder, Nina Schmid, Katja Eubler, Katharina Goetz, Annette Mueller-Taubenberger, Gregory A. Dissen, Max Harner, Gerhard Wanner, Axel Imhof, Ignasi Forne, Artur Mayerhofer
Summary: This study found that there is an increase in the number of mitochondria and intracellular trafficking of mitochondria in granulosa cells during follicular growth, which may be partially regulated by gonadotropins and cAMP. The study also discovered that cAMP profoundly affects mitochondrial dynamics in KGN cells in a PKA-dependent manner and implicates cytoskeletal changes.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Cell Biology
Jinmei Cheng, Yinchuan Li, Yan Zhang, Xiuxia Wang, Fei Sun, Yixun Liu
Summary: The study showed that the knockout of Wntless gene in oocytes in mouse ovaries did not result in any significant reproductive defects, while its deletion in granulosa cells led to reduced fertility and increased risk of miscarriage. Deletion of Wntless in granulosa cells caused noticeably smaller corpora lutea in the ovaries, reduced expression of luteal cell marker genes, increased expression of apoptotic genes, decreased levels of LHCGR and beta-Catenin proteins, and triggered granulosa cell apoptosis.
Article
Obstetrics & Gynecology
Pascal Froment, Ingrid Plotton, Cecilia Giulivi, Stephane Fabre, Rita Khoueiry, Nizar Mourad, Sandrine Horman, Christelle Rame, Charlene Rouillon, Jeremy Grandhaye, Yves Bigot, Claire Chevaleyre, Remy Le Guevel, Patricia Mallegol, Ramaroson Andriantsitohaina, Fabrice Guerif, Jerome Tamburini, Benoit Viollet, Marc Foretz, Joelle Dupont
Summary: The lack of alpha 1AMPK in granulosa cells impacts various biological processes and leads to a hyperandrogenic response.
HUMAN REPRODUCTION
(2022)
Article
Endocrinology & Metabolism
Linyi Song, Jin Yu, Danying Zhang, Xi Li, Lu Chen, Zailong Cai, Chaoqin Yu
Summary: Excess androgen could damage the mitochondrial ultrastructure and function of ovarian granulosa cells in PCOS model rats by down-regulating the expression of NDUFB8 and ATP5j.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hairui Fan, Shiqin Wang, Haifei Wang, Mingan Sun, Shenglong Wu, Wenbin Bao
Summary: These findings indicate that DON negatively impacts murine ovary GCs, while melatonin can counteract these effects by reducing oxidative stress, improving mitochondrial function, and reducing inflammation. The study sheds light on the protective effects of melatonin against mycotoxin contamination.
Article
Cell Biology
Xuan Dang, Lanlan Fang, Qiongqiong Jia, Ze Wu, Yanjie Guo, Boqun Liu, Jung-Chien Cheng, Ying-Pu Sun
Summary: This study demonstrated that TGF-beta 1 upregulates SPARC expression in the human ovary and the role of SPARC in the pathogenesis of ovarian hyperstimulation syndrome (OHSS). The activation of Slug transcription factor is required for the TGF-beta 1-induced SPARC expression. SPARC affects the occurrence of OHSS by regulating the expression of VEGF and aromatase. These findings are of great significance for the treatment of clinical infertility and OHSS.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Immunology
Qi Xie, Wenli Hong, Yuan Li, Shuyi Ling, Ziqiong Zhou, Yuqing Dai, Wenbo Wu, Ruoxin Weng, Zhisheng Zhong, Jun Tan, Yuehui Zheng
Summary: Polycystic Ovary Syndrome (PCOS) is a common reproductive endocrine disorder that can cause infertility. This study investigated the impact of inflammation and oxidative stress on granulosa cells (GCs) in PCOS patients and found that chitosan oligosaccharide (COS) can improve GC function by reducing inflammation and oxidative stress.
FRONTIERS IN IMMUNOLOGY
(2023)