Article
Agriculture, Dairy & Animal Science
Yi Zheng, Qiang Gao, Tianjiao Li, Ruifang Liu, Zechao Cheng, Ming Guo, Jinhong Xiao, De Wu, Wenxian Zeng
Summary: The study systematically investigated the development patterns of Sertoli cells and spermatogonia in pigs from neonate to sexual maturity, shedding light on previously unknown details. The findings not only expand knowledge about spermatogenesis and testicular development in pigs, but also provide a theoretical basis for porcine breeding and rearing.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Developmental Biology
Yi Jacky Peng, Xinyu Thomas Tang, Hui Sophie Shu, Wenjie Dong, Hongfang Shao, Bo O. Zhou
Summary: The study found that testicular stromal cells, including Sertoli, endothelial, Leydig, smooth muscle, and Tcf21-CreER+ stromal cells, express stem cell factor (SCF), an essential growth factor for spermatogenesis. Undifferentiated and differentiating spermatogonia were associated with SCF-expressing Sertoli cells. Conditional deletion of SCF from Sertoli cells blocked the differentiation of spermatogonia, leading to male infertility. Conditional overexpression of SCF in Sertoli cells significantly increased spermatogenesis.
Review
Obstetrics & Gynecology
Sara Di Persio, Nina Neuhaus
Summary: This review provides an overview of the increasing amount of single-cell RNA-sequencing data in the field of human male reproduction. Transcriptional profiles of thousands of testicular cells covering different developmental stages and male infertility have been generated. The review also discusses novel concepts regarding spermatogonial and somatic cell subtypes and their crosstalk, and provides corresponding marker genes for identification. Furthermore, it explores the potential clinical implications of scRNA-seq findings, the need for spatial information, and the necessity to validate results at other regulatory levels.
HUMAN REPRODUCTION
(2023)
Review
Endocrinology & Metabolism
William W. Wright
Summary: This review focuses on the in vivo regulation of spermatogonial stem cells (SSCs) in adult testes by glial cell line-derived neurotrophic factor (GDNF). Through chemical-genetic approach, the inhibition of GDNF stimulation of SSCs was found to decrease replication and increase differentiation of SSCs, ultimately leading to the loss of all SSCs and resembling the condition of Sertoli cell-only (SCO) syndrome. Abnormally low expression of GDNF, FGF2, and FGF8 was observed in infertile human testes, although some SCO testes still contain SSCs. Restoration of GDNF signaling showed partial rebuilding of depleted SSC pool, suggesting GDNF as a potential therapy for men with SCO syndrome.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Veterinary Sciences
Yaqi Cao, ZiFu Dai, Hauizhen Lao, Huimin Zhao
Summary: In this study, it was found that DMOG treatment increased the levels of HIF-1α protein and upregulated the transcription levels of SSC-specific genes in SSCs. Furthermore, DMOG treatment significantly enhanced the spermatogenesis capability of SSCs and increased the population of haploid cells.
JOURNAL OF VETERINARY SCIENCE
(2022)
Review
Endocrinology & Metabolism
Wasim Shah, Ranjha Khan, Basit Shah, Asad Khan, Sobia Dil, Wei Liu, Jie Wen, Xiaohua Jiang
Summary: The process of spermatogenesis relies on hormones and growth factors acting through endocrine and paracrine pathways. Sertoli cells are major regulators, while sex hormones play a significant role in determining their proliferation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Developmental Biology
Oleksandr Kirsanov, Taylor Johnson, Taylor Malachowski, Bryan A. Niedenberger, Emma A. Gilbert, Debajit Bhowmick, P. Hande Ozdinler, Douglas A. Gray, Kelsey Fisher-Wellman, Brian P. Hermann, Christopher B. Geyer
Summary: We have developed a facile in vitro differentiation and meiotic initiation system that can be manipulated using chemical agents that cannot be administered to live animals. Additionally, we introduced a transgenic mouse model enabling the isolation of specific developmental stages of spermatogonia and meiotic spermatocytes.
Article
Genetics & Heredity
Ke Tao, Yuan Sun, Yuanchi Chao, Liu Xing, Lizhi Leng, Dai Zhou, Wenbing Zhu, Liqing Fan
Summary: Mammalian spermatogenesis relies on the self-renewal and differentiation of spermatogonial stem cells (SSCs) with support from Sertoli cells. This study revealed that beta-estradiol can regulate the proliferation and survival of fetal SSCs by modulating the secretion of stem cell factor (SCF) in Sertoli cells, offering new insights into the role of estrogen in human spermatogenesis.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Environmental Sciences
Weiqi Sun, Fang Tian, Hongjie Pan, Xiuli Chang, Minjie Xia, Jingying Hu, Yuzhu Wang, Runsheng Li, Weihua Li, Mingjun Yang, Zhijun Zhou
Summary: This study systematically explored the male reproductive toxicity of FLC and found that it caused testicular dysfunction and damage in mice, leading to abnormal spermatogenesis and decreased sperm quality. The potential mechanisms include interference with spermatogonial proliferation and meiosis, as well as apoptosis and mitochondrial damage in testicular Sertoli cells.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Cell Biology
Liza O'Donnell, Lee B. Smith, Diane Rebourcet
Summary: Sertoli cells play essential roles in spermatogenesis, supporting germ cell development and regulating other testis somatic cells. They are crucial for maintaining the stem cell niche, facilitating meiosis and spermiogenesis, and regulating androgen production.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Review
Endocrinology & Metabolism
Maia Corpuz-Hilsabeck, Martine Culty
Summary: Sertoli cells play crucial roles in male reproduction, and dysregulation of their functions can have lifelong adverse effects on testis development and spermatogenesis. Understanding the impact of endocrine disrupting chemicals and drugs on Sertoli cells is important for comprehending the adverse outcomes on the reproductive system that these compounds may induce.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Selin Onen, Sevil Kose, Nilgun Yersal, Petek Korkusuz
Summary: This study established a co-culture system using syngeneic bone marrow-derived mesenchymal stem cells (BM-MSCs) to expand and differentiate spermatogonial stem/progenitor cells (SSPCs) in vitro, leading to improved spermatogenesis. This method has the potential to serve as an alternative treatment for male infertility.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Yoshifumi Mori, Seiji Takashima, Mito Kanatsu-Shinohara, Zheng Yi, Takashi Shinohara
Summary: The study found that Cdc42 is essential for the development of the germline niche, as its deficiency results in a significant reduction in the number of spermatogonial stem cells in the testes, affecting GDNF expression and Sertoli cell development.
Article
Cell & Tissue Engineering
Yoshiaki Nakamura, David J. Jorg, Yayoi Kon, Benjamin D. Simons, Shosei Yoshida
Summary: Tissue stem cells have the ability to regenerate host tissue following transplantation, but the dynamics of donor stem cells during regeneration are still largely unknown. Research on mouse spermatogonial stem cells showed that only a small fraction can persist and regenerate in the long term, while the rest are lost through differentiation and cell death. By transiently suppressing differentiation, repopulation efficiency can be increased to restore the fertility of infertile hosts.
Article
Reproductive Biology
Kazem Rasouli-Gharehsaghal, Malak Shakeri, Mahdi Zhandi, Hamid-Reza Amini, Fereshteh Ghadimi, Afsaneh Golkar-Narenji, Paul Edward Mozdziak
Summary: The study aimed to investigate the feasibility of regenerating spermatogenesis in infertile roosters by transplanting transfected SSCs into testes. The results showed that transfected SSCs transplanted into testes could lead to the detection of transgenic material in the roosters' spermatozoa and offspring. However, no transgenic offspring were found through mating and PCR testing.
Article
Cell Biology
Minori Shinya, Kayo Kobayashi, Aki Masuda, Mika Tokumoto, Yuichi Ozaki, Kenji Saito, Toshihiro Kawasaki, Yukiko Sado, Noriyoshi Sakai
DEVELOPMENT GROWTH & DIFFERENTIATION
(2013)
Article
Anatomy & Morphology
Kenji Saito, Kellee R. Siegfried, Christiane Nuesslein-Volhard, Noriyoshi Sakai
DEVELOPMENTAL DYNAMICS
(2011)
Article
Anatomy & Morphology
Kenji Saito, Chiharu Sakai, Toshihiroa Kawasaki, Noriyoshi Sakai
DEVELOPMENTAL DYNAMICS
(2014)
Article
Developmental Biology
Yuichi Ozaki, Kenji Saito, Minori Shinya, Toshihiro Kawasaki, Noriyoshi Sakai
GENE EXPRESSION PATTERNS
(2011)
Article
Cell Biology
Toshihiro Kawasaki, Kenji Saito, Chiharu Sakai, Minori Shinya, Noriyoshi Sakai
Article
Developmental Biology
Toshihiro Kawasaki, Kenji Saito, Kaoru Mitsui, Masahito Ikawa, Masakane Yamashita, Yoshihito Taniguchi, Shunichi Takeda, Kohnosuke Mitani, Noriyoshi Sakai
Article
Genetics & Heredity
Kazumasa Takemoto, Yukiko Imai, Kenji Saito, Toshihiro Kawasaki, Peter M. Carlton, Kei-ichiro Ishiguro, Noriyoshi Sakai
Article
Immunology
Masayoshi Aosasa, Md Saddam Hossain, Tomoko Sakata, Keita Koga, Takanari Shigemitsu, Yuji Shoya, Motonori Yamaguchi, Kenji Saito, Mujo Kim
Summary: A specific epitope of PAD2 was identified as an antigenic site, and antibodies generated through immunization showed strong binding capabilities, effectively inhibiting the PAD2-catalyzed citrullination reaction. These antibodies demonstrated powerful inhibition of citrullination reaction, which could have potential therapeutic implications in treating related diseases.
JOURNAL OF IMMUNOLOGY RESEARCH
(2021)
Article
Cell Biology
Yukiko Imai, Kenji Saito, Kazumasa Takemoto, Fabien Velilla, Toshihiro Kawasaki, Kei-ichiro Ishiguro, Noriyoshi Sakai
Summary: The mutation in the zebrafish gene encoding the transverse filament protein Sycp1 leads to incomplete pairing of homologs in zebrafish meiosis, while not affecting double-strand break formation near telomeres.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)