期刊
ANALYTICA CHIMICA ACTA
卷 887, 期 -, 页码 51-58出版社
ELSEVIER
DOI: 10.1016/j.aca.2015.05.048
关键词
Legionella pneumophila; Magnetic nanoparticles; poly(dopamine); Electrochemical immunoassay
资金
- program Avanza I+D Competitividad Ministerio de Industria Turismo y Comercio [TSI-020100-2011-189]
- FEDER
- Ministry of Economy and Competitiveness
- Spanish Ministerio de Economia y Competitividad [CTQ2011-24355, CTQ2012-34238]
- Comunidad de Madrid [S2013/MT-3029]
A novel amperometric magnetoimmunoassay, based on the use of core-shell magnetic nanoparticles and screen-printed carbon electrodes, was developed for the selective determination of Legionella pneumophila SG1. A specific capture antibody (Ab) was linked to the poly(dopamine)-modified magnetic nanoparticles (MNPs@pDA-Ab) and incubated with bacteria. The captured bacteria were sandwiched using the antibody labeled with horseradish peroxidase (Ab-HRP), and the resulting MNPs@pDA-Ab-Legionella neumophila-Ab-HRP were captured by a magnetic field on the electrode surface. The amperometric response measured at -0.15 V vs. Ag pseudo-reference electrode of the SPCE after the addition of H2O2 in the presence of hydroquinone (HQ) was used as transduction signal. The achieved limit of detection, without pre-concentration or pre-enrichment steps, was 10(4) Colony Forming Units (CFUs) m L-1. The method showed a good selectivity and the MNPs@pDA-Ab exhibited a good stability during 30 days. The possibility of detecting L. pneumophila at 10 CFU mL(-1) level in less than 3 h, after performing a membrane-based preconcentration step, was also demonstrated. (C) 2015 Published by Elsevier B.V.
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